Waste Water Derived Electroactive Microbial Biofilms: Growth, Maintenance, and Basic Characterization

Gimkiewicz, Carla

doi:10.3791/50800-v

Cited by 3 publications

(8 citation statements)

References 13 publications

(18 reference statements)

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“…biofilms in differently pretreated AD-effluent over four successive batch cycles, denoted B1, B2, B3, and B4, with one batch cycle lasting always 1 week. Referring to previous work , and our own biofilm pre-growth (data not shown), 3-week-old and 6-week-old Geobacter spp. biofilms were considered to be in a steady state.…”

Section: Methodssupporting

confidence: 81%

“…Biofilms were subsequently enriched using a simple electrochemical enrichment procedure according to Liu et al 41 The used growth medium consisted of 50 mmol L −1 phosphate buffer, amended with 10 mmol L −1 sodium acetate, vitamins, and trace elements. 40,42 For more details, please see the Supporting Information. AD-Effluent.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

“…Geobacter spp. biofilm inoculum was initially grown according to Gimkiewicz et al using wastewater from a primary clarifier of a local wastewater treatment plant (AZV Parthe, 04551 Borsdorf, Germany). Biofilms were subsequently enriched using a simple electrochemical enrichment procedure according to Liu et al The used growth medium consisted of 50 mmol L –1 phosphate buffer, amended with 10 mmol L –1 sodium acetate, vitamins, and trace elements. , For more details, please see the Supporting Information.…”

Section: Methodsmentioning

confidence: 99%

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Combining Geobacter spp. Dominated Biofilms and Anaerobic Digestion Effluents─The Effect of Effluent Composition and Electrode Potential on Biofilm Activity and Stability

Ngoumelah

Kuchenbuch

Kretzschmar

2023

Environ. Sci. Technol.

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The combination of anaerobic digestion (AD) and microbial electrochemical technologies (METs) offers different opportunities to increase the efficiency and sustainability of AD processes. However, methanogenic archaea and/or particles may partially hinder combining MET and AD processes. Furthermore, it is unclear if the applied anode potential affects the activity and efficiency of electroactive microorganisms in AD-MET combinations as it is described for more controlled experimental conditions. In this study, we confirm that 6-week-old Geobacter spp. dominated biofilms are by far more active and stable in AD-effluents than 3-week-old Geobacter spp. dominated biofilms. Furthermore, we show that the biofilms are twice as active at −0.2 V compared to 0.4 V, even under challenging conditions occurring in AD-MET systems. Paired-end amplicon sequencing at the DNA level using 16S-rRNA and mcrA gene shows that hydrogenotrophic methanogens incorporate into biofilms immersed in AD-effluent without any negative effect on biofilm stability and electrochemical activity.

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Section: Methodssupporting

confidence: 81%

Section: ■ Materials and Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Combining Geobacter spp. Dominated Biofilms and Anaerobic Digestion Effluents─The Effect of Effluent Composition and Electrode Potential on Biofilm Activity and Stability

Ngoumelah

Kuchenbuch

Kretzschmar

2023

Environ. Sci. Technol.

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“…Two reactor replicates were inoculated and operated under chronoamperometry mode without aeration. The working electrode was poised at +0.2 V vs. Ag/AgCl following the standardised Geobacter methodology (model anode-respiring bacteria) [ 18 ]. After 21 days of operation, aeration was introduced into the system at a periodicity of 1 h per day.…”

Section: Methodsmentioning

confidence: 99%

“…Microbial electrochemical technologies (METs) are potential alternatives to suppress aeration in wastewater treatment plants due to the ability of some microorganisms to use an electrode as an electron acceptor instead of oxygen [ 1 , 8 , 18 ]. Considering the pivoting role of hydroxylamine in electricity-driven nitrification [ 5 , 8 ], one might hypothesise those heterotrophic nitrifying microorganisms such as Alcaligenes , could also be able to use an electrode as the terminal electron acceptor.…”

Section: Introductionmentioning

confidence: 99%

Direct ammonium oxidation to nitrogen gas (Dirammox) in Alcaligenes strain HO-1: The electrode role

Pous¹,

Bañeras²,

Corvini³

et al. 2023

Environmental Science and Ecotechnology

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Benchmark and strategy development for microbial fuel cells in industrial wastewater treatment

Brunschweiger¹,

Hofmann²

2023

Lebensmittelchemie

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The chemical energy hidden in wastewater can be extracted, turning an energy‐intensive treatment process into an energy‐independent one. However, conventional aerobic treat‐ ment is very energy intensive, and anaerobic treatment requires a post‐treatment step to meet stringent discharge requirements. Therefore, Microbial Fuel Cells (MFCs) have attracted a lot of attention because of their ability to extract electrical energy directly from wastewater during the treatment process. Since combustion losses can be avoided, theoretically the greatest energy value can be obtained from the organic load. However, most MFC research is currently still taking place on a laboratory scale in the treatment of synthetic or municipal wastewater. Commercialization of MFC technology will require large‐scale plants, for which a suitable MFC design and operation concepts must first be identified, since neither configuration nor operating system has been clearly established yet. In addition, no specific concepts and application fields currently exist for the treat‐ ment of industrial wastewater.Consequently, with regard to MFCs in industrial wastewater treatment, the aim of this work is to develop a benchmark that serves for modeling the required overall efficiency of MFCs and to identify the most relevant key factors in order to derive enhancement strate‐ gies. By providing an overview of current MFCs in industrial wastewater treatment and developing a benchmark, the targets for long‐term operation of MFCs can be established allowing critical factors for design and operation to be identified. The resulting enhance‐ ment strategies were validated and the overall evaluation with the developed benchmark allowed an assessment regarding the commercialization potential.Compared to the first MFC design (MFC 1.0), the enhanced MFC design (MFC 2.0) increased the power density by a factor of up to 11 and extended the long‐term stability to one year by increasing the specific cathode surface area and reducing the electrode spacing in conjunction with avoiding fiber clogging on the anode side. In addition to using beneficial brewery wastewater with high content of easily degradable organic acids and high conductivity, the performance of the MFC was further stabilized and improved by changing the operating mode to continuous operation and reducing the hydraulic re‐ tention time to 6 h, resulting in a mean organic removal rate of 6.5 ± 1.9 kg/(m3 · d). Although the overall energy efficiency is low compared to anaerobic treatment, the enor‐ mous wastewater treatment potential forms the basis for MFCs to become an alternative to conventional treatment technologies if self‐sufficient treatment is targeted. Due to the wide range of operating conditions and the modularity of stack systems, MFCs can become a promising option especially for industrial wastewater treatment.

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Waste Water Derived Electroactive Microbial Biofilms: Growth, Maintenance, and Basic Characterization

Cited by 3 publications

References 13 publications

Combining Geobacter spp. Dominated Biofilms and Anaerobic Digestion Effluents─The Effect of Effluent Composition and Electrode Potential on Biofilm Activity and Stability

Combining Geobacter spp. Dominated Biofilms and Anaerobic Digestion Effluents─The Effect of Effluent Composition and Electrode Potential on Biofilm Activity and Stability

Direct ammonium oxidation to nitrogen gas (Dirammox) in Alcaligenes strain HO-1: The electrode role

Benchmark and strategy development for microbial fuel cells in industrial wastewater treatment

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