Waste to bioproduct conversion with undefined mixed cultures: the carboxylate platform

Agler, Matthew T.; Wrenn, Brian A.; Zinder, Stephen H.; Angenent, Largus T.

doi:10.1016/j.tibtech.2010.11.006

Cited by 705 publications

(565 citation statements)

References 70 publications

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“…Since the decomposition of plant matter is achieved via a complex mixed microbial community [45], a bottleneck at the top of this microbial food web would result in retarded downstream processes (e.g., fermentation, iron reduction, and methanogenesis). If either nonpoised or poised electrodes stimulated the breakdown of nondegraded organic material through syntrophic product removal (i.e., increasing the consumption of inhibitory products), the bottleneck at the top of the microbial food web would have widened, resulting in a larger carbon flux through the ecosystem.…”

Section: Changes In Methane Emissions Correspond To Changes In Microbmentioning

confidence: 99%

Potentiostatically Poised Electrodes Mimic Iron Oxide and Interact with Soil Microbial Communities to Alter the Biogeochemistry of Arctic Peat Soils

et al. 2013

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Dissimilatory metal-reducing bacteria are ubiquitous in soils worldwide, possess the ability to transfer electrons outside of their cell membranes, and are capable of respiring with various metal oxides. Reduction of iron oxides is one of the more energetically favorable forms of anaerobic respiration, with a higher energy yield than both sulfate reduction and methanogenesis. As such, this process has significant implications for soil carbon balances, especially in the saturated, carbon-rich soils of the northern latitudes. However, the dynamics of these microbial processes within the context of the greater soil microbiome remain largely unstudied. Previously, we have demonstrated the capability of potentiostatically poised electrodes to mimic the redox potential of iron(III)-and humic acid-compounds and obtain a measure of metal-reducing respiration. Here, we extend this work by utilizing poised electrodes to provide an inexaustable electron acceptor for ironand humic acid-reducing microbes, and by measuring the effects on both microbial community structure and greenhouse gas emissions. The application of both nonpoised and poised graphite electrodes in peat soils stimulated methane emissions by 15%-43% compared to soils without electrodes. Poised electrodes resulted in higher (13%-24%) methane emissions than the nonpoised electrodes. The stimulation of methane emissions for both nonpoised and poised electrodes correlated with the enrichment of proteobacteria, verrucomicrobia, and bacteroidetes. Here, we demonstrate a tool for precisely manipulating OPEN ACCESSMinerals 2013, 3 319 localized redox conditions in situ (via poised electrodes) and for connecting microbial community dynamics with larger ecosystem processes. This work provides a foundation for further studies examining the role of dissimilatory metal-reducing bacteria in global biogeochemical cycles.

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Section: Changes In Methane Emissions Correspond To Changes In Microbmentioning

confidence: 99%

Potentiostatically Poised Electrodes Mimic Iron Oxide and Interact with Soil Microbial Communities to Alter the Biogeochemistry of Arctic Peat Soils

et al. 2013

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“…Microbial conversion approaches, often enabled by the use of strains that naturally accumulate products of interest, have garnered significant interest in the emerging bioeconomy. One such strategy, the "Carboxylate Platform"-namely the production of volatile fatty acids (VFAs, specifically C2-C8 VFAs) from biomass sources via anaerobic fermentation either through mixed cultures or mono-culture-has emerged as an attractive approach to generate high yields of promising biofuel and biochemical precursors [1,2]. Most well studied VFA-producing microbes employ anaerobic chain elongation [3,4].…”

Section: Introductionmentioning

confidence: 99%

Mixed Carboxylic Acid Production by Megasphaera elsdenii from Glucose and Lignocellulosic Hydrolysate

et al. 2017

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Volatile fatty acids (VFAs) can be readily produced from many anaerobic microbes and subsequently utilized as precursors to renewable biofuels and biochemicals. Megasphaera elsdenii represents a promising host for production of VFAs, butyric acid (BA) and hexanoic acid (HA). However, due to the toxicity of these acids, product removal via an extractive fermentation system is required to achieve high titers and productivities. Here, we examine multiple aspects of extractive separations to produce BA and HA from glucose and lignocellulosic hydrolysate with M. elsdenii. A mixture of oleyl alcohol and 10% (v/v) trioctylamine was selected as an extraction solvent due to its insignificant inhibitory effect on the bacteria. Batch extractive fermentations were conducted in the pH range of 5.0 to 6.5 to select the best cell growth rate and extraction efficiency combination. Subsequently, fed-batch pertractive fermentations were run over 230 h, demonstrating high BA and HA concentrations in the extracted fraction (57.2 g/L from~190 g/L glucose) and productivity (0.26 g/L/h). To our knowledge, these are the highest combined acid titers and productivity values reported for M. elsdenii and bacterial mono-cultures from sugars. Lastly, the production of BA and HA (up to 17 g/L) from lignocellulosic sugars was demonstrated.

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“…The carboxylate platform is comprised of biological and chemical pathways that can be used in order to convert waste to bioproducts, such as lactic acid [17]. Lactic acid is a relatively cheap product, and one of the major challenges in its large-scale fermentative production is the cost of the raw material.…”

Section: Future Raw Materials For Production Of Lactic Acid By Labmentioning

confidence: 99%

The Current Status and Future Expectations in Industrial Production of Lactic Acid by Lactic Acid Bacteria

Taskila¹,

Ojamo²

2013

Lactic Acid Bacteria - R &Amp; D for Food, Health and Livestock Purposes

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Waste to bioproduct conversion with undefined mixed cultures: the carboxylate platform

Cited by 705 publications

References 70 publications

Potentiostatically Poised Electrodes Mimic Iron Oxide and Interact with Soil Microbial Communities to Alter the Biogeochemistry of Arctic Peat Soils

Potentiostatically Poised Electrodes Mimic Iron Oxide and Interact with Soil Microbial Communities to Alter the Biogeochemistry of Arctic Peat Soils

Mixed Carboxylic Acid Production by Megasphaera elsdenii from Glucose and Lignocellulosic Hydrolysate

The Current Status and Future Expectations in Industrial Production of Lactic Acid by Lactic Acid Bacteria

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