Washing packed red blood cells decreases red blood cell storage lesion formation

Pulliam, Kasiemobi E.; Joseph, Bernadin; Makley, Amy T.; Caldwell, Charles C.; Lentsch, Alex B.; Goodman, Michael D.; Pritts, Timothy A.

doi:10.1016/j.surg.2020.07.022

Cited by 7 publications

(9 citation statements)

References 22 publications

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“…It was demonstrated that the supplementation of RBCs stored with uric acid and ascorbic acid, both antioxidant molecules, reduces oxidative damage and induces GSH synthesis ( Tzounakas et al, 2022 ; Anastasiadi et al, 2023 ). Furthermore, washing stored RBCs with PBS seems to reduce senescence pathways, phosphatidylserine and band-3 exposure, and membrane fragility ( Pulliam et al, 2021 ). D’Alessandro et al proved that hypoxic storage conditions improved 2,3-BPG and GSH synthesis, and ameliorated redox metabolism ( DʼAlessandro et al, 2020 ).…”

Section: How We Can Take Advantage In Converting Rbcs Into a Drug Car...mentioning

confidence: 99%

Drug transport by red blood cells

Biagiotti,

Perla,

Magnani

2023

Front. Physiol.

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This review focuses on the role of human red blood cells (RBCs) as drug carriers. First, a general introduction about RBC physiology is provided, followed by the presentation of several cases in which RBCs act as natural carriers of drugs. This is due to the presence of several binding sites within the same RBCs and is regulated by the diffusion of selected compounds through the RBC membrane and by the presence of influx and efflux transporters. The balance between the influx/efflux and the affinity for these binding sites will finally affect drug partitioning. Thereafter, a brief mention of the pharmacokinetic profile of drugs with such a partitioning is given. Finally, some examples in which these natural features of human RBCs can be further exploited to engineer RBCs by the encapsulation of drugs, metabolites, or target proteins are reported. For instance, metabolic pathways can be powered by increasing key metabolites (i.e., 2,3-bisphosphoglycerate) that affect oxygen release potentially useful in transfusion medicine. On the other hand, the RBC pre-loading of recombinant immunophilins permits increasing the binding and transport of immunosuppressive drugs. In conclusion, RBCs are natural carriers for different kinds of metabolites and several drugs. However, they can be opportunely further modified to optimize and improve their ability to perform as drug vehicles.

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Section: How We Can Take Advantage In Converting Rbcs Into a Drug Car...mentioning

confidence: 99%

Drug transport by red blood cells

Biagiotti,

Perla,

Magnani

2023

Front. Physiol.

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“…Hemolysis rates (%) of both blank-packed red blood cells and RBC@SIM-PEI-PPNPs were determined by suspending them in solutions of different NaCl concentrations and examining their osmotic fragility. 36 Firstly, prepare various concentration gradients of NaCl solutions. Take 5 mL of each concentration gradient of NaCl solution and add it to a 15 mL centrifuge tube.…”

Section: Methodsmentioning

confidence: 99%

Red Blood Cell-Hitchhiking Delivery of Simvastatin to Relieve Acute Respiratory Distress Syndrome

Sun,

Wei,

et al. 2024

IJN

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“…sREVs significantly decreased up to 2 h after washing PRBCs using an automated cell saver with a low centrifugal force [23]. sREVs also decreased in stored murine RBCs after mid-storage washing at 7 days with an additional 7 day storage in AS1 compared with 14 days of storage without washing [24]. In humans, washed RBCs should be transfused within 24 h when conserved at 1°C–6°C.…”

Section: Generation and Characterization Of Stored Red Cell Derived E...mentioning

confidence: 96%

Red cell extracellular vesicles and coagulation activation pathways

Noubouossie,

Key

2023

Current Opinion in Hematology

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Purpose of review Packed red blood cells (PRBCs) are the most commonly transfused blood products. Preparation of PRBCs requires blood collection from donors, processing, and storage prior to transfusion to recipients. Stored red blood cells (RBCs) undergo structural and metabolic changes collectively known as the storage lesion. RBC extracellular vesicles (sREVs) are released in PRBC units during storage, and are transfused along with intact RBCs into recipients. For several decades, extracellular vesicles have been the focus of intense research, leading to the discovery of a wide variety of endogenous biological properties that may impact numerous physiologic and/or pathologic pathways. Recent findings This study reviews the characteristics of extracellular vesicles present in PRBC units and the impact of prestorage and pretransfusion processing, as well as storage conditions, on their generation. Importantly, we discuss recently described interactions of sREVs with coagulation pathways and related interplay with inflammatory pathways in vitro and in vivo using animal models. Summary Extracellular vesicles present in stored PRBC units are capable of activating coagulation pathways. However, it remains unclear whether this affects clinical outcomes in recipients of PRBC units. Further understanding of these pathways and their relationship to any adverse outcomes may yield novel strategies to mitigate complications of blood transfusion.

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Washing packed red blood cells decreases red blood cell storage lesion formation

Cited by 7 publications

References 22 publications

Drug transport by red blood cells

Drug transport by red blood cells

Red Blood Cell-Hitchhiking Delivery of Simvastatin to Relieve Acute Respiratory Distress Syndrome

Red cell extracellular vesicles and coagulation activation pathways

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