Washing of platelets can be fully automated using a closed‐system cell processor and BRS‐A platelet additive solution

Abstract

This study evaluated the in vitro properties of platelets (PLTs) washed with BRS-A additive solution in the Haemonetics ACP215 automated processing system. Two washing modes, 'manually/automatically adding ACD-A to BRS before/during the washing process', represented the control and test groups, respectively. Outcomes were compared over 7 days of storage (n = 7, for both). PLT recovery following washing processing (26-27 min) was 86·2 ± 1·7% and 86·0 ± 2·2% and plasma protein removal was 98·8 ± 0·3% and 99·0 ± 0·2% in the control and test groups, respectively (not significant). Both groups exhibited comparable in vitro properties.

Impact of the platelet washing process on in vitro platelet properties, and the levels of soluble CD40 ligand and platelet‐derived microparticles in the storage media

“…In Study 2, PLTs recovered and washed with a manual method and ACP215 system were 94.1 AE 3.8 and 90.0 AE 2.3%, respectively, which were comparable to those obtained in our previous study 12,19,20 and in previous reports examining automatic washing with COBE 2991, [14][15][16][17] except for the hollowfiber column system. 18 Decreased PLT recovery was reported to be attributed to the occurrence of PLT clumping by the washing process.…”

“…Once the procedure is started, BRS-A additive solution is prepared by automatically mixing BRS and ACD-A in a ratio of 20:1, and washed PLTs can be obtained in the product bag without residual PLTs in a separation bowl. 20 The Japanese Red Cross Society obtained manufacturing and marketing approval for washed PLT products from the Ministry of Health, Labor and Welfare in Japan in March 2016 and began distribution in September 2016. 21 Several immunomodulatory factors that can accumulate in the storage media, such as soluble CD40 ligand (sCD40L) and platelet-derived microparticles (PDMPs), may lead to platelet transfusion reactions.…”

“…Decreased PLT recovery was reported to be attributed to the occurrence of PLT clumping by the washing process . In ACP215‐based washing, PLT clumps can completely disappear within a few minutes . One explanation for this phenomenon might be the presence of a sufficient amount of anticoagulant in BRS‐A additive solution and the appropriate physical stress in the ACP215 processing system.…”

“…Recently, this program has been improved to achieve full automation. Once the procedure is started, BRS‐A additive solution is prepared by automatically mixing BRS and ACD‐A in a ratio of 20:1, and washed PLTs can be obtained in the product bag without residual PLTs in a separation bowl . The Japanese Red Cross Society obtained manufacturing and marketing approval for washed PLT products from the Ministry of Health, Labor and Welfare in Japan in March 2016 and began distribution in September 2016 …”

48‐hour interruption of agitation: effect on quality of washed PLT suspended in BRS‐A

“…The residual plasma level of this product, which is prepared using an automated cell processor (ACP215, Haemonetics Corp.) is approximately 1%, and PLT properties can be preserved in vitro for at least 7 days. 1,2 Recently, a retrospective multicenter study reported that this product was effective and safe for prevention of recurrent or severe transfusion reactions. 3 PLT products are generally stored with continuous agitation to maintain their quality; however, the interruption of agitation for 24 hours has only a slight impact on the in vitro properties of washed PLTs.…”