Warm Ischemia in Transplantation: Search for a Consensus Definition

Halazun, Karim J.; Al-Mukhtar, A. M.; Aldouri, Amer; Willis, Sean; Ahmad, Niaz

doi:10.1016/j.transproceed.2007.02.061

Cited by 78 publications

(56 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…2). There is no universal consensus regarding the definition of DWIT [39]. Originally DWIT was defined as the time from cardiac arrest until the start of hypothermic preservation [40].…”

Section: Donor Factorsmentioning

confidence: 99%

“…Originally DWIT was defined as the time from cardiac arrest until the start of hypothermic preservation [40]. Although this definition allowed for consistency, it was subsequently changed to the more widely used definition which marks the time interval from withdrawal of cardiopulmonary support till start of hypothermic preservation [39]. Adding further complexity, other groups attempt to define DWIT as beginning at the agonal period when oxygen saturation declines and/or tissue perfusion is compromised by hypotension [41].…”

Section: Donor Factorsmentioning

confidence: 99%

See 1 more Smart Citation

Liver Transplantation Using Grafts from Donation After Cardiac Death Donors

2015

View full text Add to dashboard Cite

Utilization of liver grafts from donation after cardiac death donors has been proposed as a potential solution to the organ shortage for patients suffering from end-stage liver disease. The number of donation after cardiac death donors is likely to increase as a consequence of changing demographics and improvements in neurologic care. This potential has not been realized to full effect due to reports of inferior outcomes of liver transplantation using these grafts. In order to continue to provide excellent transplant care, it is critical to have an understanding of the factors necessary for successful use of liver grafts from donation after cardiac death donors. In this review, we will appraise the current understanding of the challenges involved in using these grafts, the critical recipient and donor considerations, and future directions.

show abstract

“…2). There is no universal consensus regarding the definition of DWIT [39]. Originally DWIT was defined as the time from cardiac arrest until the start of hypothermic preservation [40].…”

Section: Donor Factorsmentioning

confidence: 99%

Section: Donor Factorsmentioning

confidence: 99%

Liver Transplantation Using Grafts from Donation After Cardiac Death Donors

2015

View full text Add to dashboard Cite

show abstract

“…The goal of organ preservation is to develop a method to monitor the biomarkers of stress induced by the procurement and preservation process in real-time in order to extend the preservation period to allow for assembly of the surgical team and the transport of the organ between facilities. The typical preservation time for organs is anywhere from 15 to 30 h [3]. The time for the organs to remain viable depends on the organ type, as illustrated in Figure 1.…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, when an individual experiences an extremity limb loss, the flow of nutrients and oxygen to that limb ceases, which initiates the deterioration of the limb's tissue. This process is described as ischemia [3]. Ischemia can be identified by the coupled and accelerated production of lactic acid and oxidation of glucose due to elevated glycolysis in the oxygen-deprived tissue [4].…”

Section: Introductionmentioning

confidence: 99%

Self-Powered Electrochemical Lactate Biosensing

Baingane

Slaughter

2017

Energies

View full text Add to dashboard Cite

This work presents the development and characterization of a self-powered electrochemical lactate biosensor for real-time monitoring of lactic acid. The bioanode and biocathode were modified with D-lactate dehydrogenase (D-LDH) and bilirubin oxidase (BOD), respectively, to facilitate the oxidation and reduction of lactic acid and molecular oxygen. The bioelectrodes were arranged in a parallel configuration to construct the biofuel cell. This biofuel cell's current-voltage characteristic was analyzed in the presence of various lactic acid concentrations over a range of 1-25 mM. An open circuit voltage of 395.3 mV and a short circuit current density of 418.8 µA/cm 2 were obtained when operating in 25 mM lactic acid. Additionally, a 10 pF capacitor was integrated via a charge pump circuit to the biofuel cell to realize the self-powered lactate biosensor with a footprint of 1.4 cm × 2 cm. The charge pump enabled the boosting of the biofuel cell voltage in bursts of 1.2-1.8 V via the capacitor. By observing the burst frequency of a 10 pF capacitor, the exact concentration of lactic acid was deduced. As a self-powered lactate sensor, a linear dynamic range of 1-100 mM lactic acid was observed under physiologic conditions (37 • C, pH 7.4) and the sensor exhibited an excellent sensitivity of 125.88 Hz/mM-cm 2 . This electrochemical lactate biosensor has the potential to be used for the real-time monitoring of lactic acid level in biological fluids.

show abstract

“…The duration of ischemia plays a crucial role in obtaining viable cells from postmortem tissues [Hwang et al, 2005;Międzybrodzki et al, 2006;Halazun et al, 2007]. The pathological mechanism of ischemia involves inflammation, glial inactivation, and programmed cell death.…”

Section: Introductionmentioning

confidence: 99%

The Impact of Oxidative Stress Factors on the Viability, Senescence, and Methylation Status of Olfactory Bulb-Derived Glial Cells Isolated from Human Cadaver Donors

Marycz

Kornicka²,

Grzesiak³

et al. 2017

Cells Tissues Organs

View full text Add to dashboard Cite

The olfactory bulb (OB) is a unique structure in the central nervous system that retains the ability to create new neuronal connections. Glial cells isolated from the OB have been recently considered as a novel and promising tool to establish an effective therapy for central nervous system injuries. Due to the hindered access to autologous tissue for cell isolation, an allogeneic source of tissues obtained postmortem has been proposed. In this study, we focused on the morphological and molecular characteristics of human OB-derived glial cells isolated postmortem, at different time points after a donor's death. We evaluated the proliferative activity of the isolated cells, and investigated the ultrastructure of the mitochondria, the accumulation of intracellular reactive oxygen species, and the activity of superoxide dismutase. The data obtained clearly indicate that the duration of ischemia is crucial for the viability/senescence rate of OB-derived glial cells. The OB can be isolated during autopsy and still stand as a source of viable glial cells, but ischemia duration is a major factor limiting its potential usefulness in therapies.

show abstract

Warm Ischemia in Transplantation: Search for a Consensus Definition

Cited by 78 publications

References 13 publications

Liver Transplantation Using Grafts from Donation After Cardiac Death Donors

Liver Transplantation Using Grafts from Donation After Cardiac Death Donors

Self-Powered Electrochemical Lactate Biosensing

The Impact of Oxidative Stress Factors on the Viability, Senescence, and Methylation Status of Olfactory Bulb-Derived Glial Cells Isolated from Human Cadaver Donors

Contact Info

Product

Resources

About