Wangella harbinensis gen. nov., sp. nov., a new member of the family Micromonosporaceae

Jia, Feiyu; Liu, Chongxi; Wang, Xiangjing; Zhao, Junwei; Liu, Qianfeng; Ji, Zhang; Gao, Ruize; Xiang, Wensheng

doi:10.1007/s10482-012-9820-1

Cited by 100 publications

(45 citation statements)

References 48 publications

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“…Biomass for chemical studies were prepared by growing strain 3H-GS17 T in modified GY (Jia et al, 2013) medium in shake flasks at 28 C for 10 days. Cells were harvested by centrifugation, washed with distilled water and freeze-dried.…”

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Actinocorallia lasiicapitis sp. nov., an actinomycete isolated from the head of an ant (Lasius fuliginosus L.)

Liu

Yao

et al. 2016

International Journal of Systematic and Evolutionary Microbiology

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A novel actinomycete, designated strain 3H-GS17 T , was isolated from the head of an ant (Lasius fuliginosus L.) and characterized using a polyphasic approach. 16S rRNA gene sequence similarity studies showed that strain 3H-GS17 T belongs to the genus Actinocorallia with high sequence similarity to Actinocorallia glomerata JCM 9376 T (98.13 %) and Actinocorallia longicatena JCM 9377 T (97.64 %). The chemotaxonomic properties of strain 3H-GS17 T were also consistent with those of members of the genus Actinocorallia. The cell wall contained mesodiaminopimelic acid and whole-cell sugars were ribose, mannose, glucose, galactose and madurose. The predominant menaquinones were MK-9(H 4 ), MK-9(H 6 ) and MK-9(H 8 ). The phospholipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannoside. The major fatty acids were C 16 : 0 and C 18 : 1 !7с. A combination of DNA-DNA hybridization experiments and phenotypic tests were carried out between strain 3H-GS17 T and its closely related strains, which further clarified their relatedness and demonstrated that 3H-GS17 T could be distinguished from these strains. Therefore, strain 3H-GS17 T is concluded to represent a novel species of the genus Actinocorallia, for which the name Actinocorallia lasiicapitis sp. nov. is proposed. The type strain is 3H-GS17 T (=DSM 100595 T =CGMCC 4.7282 T ).The genus Actinocorallia was first proposed by Iinuma et al. (1994) as a member of the family Thermomonosporaceae and its description was emended by Zhang et al. (2001). Members of the genus form spore chains on the aerial mycelium. The genus is characterized chemotaxonomically by the presence of meso-diaminopimelic acid in the cell wall, diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol as the major phospholipids, MK-9 (H 4 ), MK-9(H 6 ) and MK-9(H 8 ) as the predominant menaquinones, and hexadecanoic, 14-methylpentadecanoic, octadecanoic and 19-methyloctadecanoic acids as the predominant fatty acids (Trujillo & Goodfellow, 2012). At the time of writing, the genus comprises seven species with validly published names (http://www.bacterio.net/actinocorallia.html). During a study of the diversity of symbiotic actinomycetes in ants, strain 3H-GS17 T was isolated from the head of Lasius fuliginosus L. In this study, we performed a polyphasic taxonomic analysis on this strain, and propose that it is a representative of a novel species of the genus Actinocorallia.Strain 3H-GS17 T was isolated from the head of L. fuliginosus L. collected from Northeast Agriculture University (Harbin, Heilongjiang, China). L. fuliginosus individuals were caught under a willow tree adjacent to their formicary. Ten individuals were surface-disinfected in 70 % ethanol for 60 s and then washed three times in sterile distilled water. Surface-disinfected individual ants were divided into head, mesosoma and gaster, and each body part was separately put in 500 µl of sterile water with shaking on a rotary shaker at 180 r.p.m at 28 C for ...

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confidence: 99%

Actinocorallia lasiicapitis sp. nov., an actinomycete isolated from the head of an ant (Lasius fuliginosus L.)

Liu

Yao

et al. 2016

International Journal of Systematic and Evolutionary Microbiology

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“…Blastococcus aggregatus Data for reference genera were taken from Li et al (2011), Thawai et al (2006, Couch (1950), Lee & Lee (2011), Lee & Hah (2002), Asano & Kawamoto (1986), Ara et al (2008a, b), Yokota et al (1993), Tamura et al (1994Tamura et al ( , 1997Tamura et al ( , 2001Tamura et al ( , 2006, Thiemann et al (1967), Xie et al (2011), Ara & Kudo, (2007a, Matsumoto et al (2003Matsumoto et al ( , 2014, Ørskov (1923), Inahashi et al (2010), Kane (1966), Wiese et al (2008), Qin et al (2009), Monciardini et al (2009), Maldonado et al (2005, Rheims et al (1998), Jia et al (2013) and Wang et al (2013). +, Present; À, absent.…”

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confidence: 99%

Actinorhabdospora filicis gen. nov., sp. nov., a new member of the family Micromonosporaceae

Mingma

Tanaka

Ōmura

et al. 2016

International Journal of Systematic and Evolutionary Microbiology

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The actinomycete strains K12-0408 T and K12-0792 were isolated on CM-cellulose agar from rhizosphere soil of a pteridophytic plant collected in Tokyo prefecture, Japan. Their taxonomic positions were determined using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strains K12-0408 T and K12-0792 were positioned within the family Micromonosporaceae. The strains formed extensively branched aerial and substrate mycelia. Long chains of cylindrical spores with smooth surfaces were formed on aerial hyphae. The cell wall contained meso-diaminopimelic acid, and galactose, glucose, mannose and ribose were detected in whole-cell hydrolysates. The predominant menaquinones were MK-10(H 4 ) and MK-10(H 6 ). The major cellular fatty acids were anteiso-C 17 : 0 and anteiso-C 17 : 0 2-OH. The DNA G+C contents of strains K12-0408 T and K12-0792 were 69.6 and 69.7 mol%, respectively. Based on data from the present polyphasic taxonomic study, strains K12-0408 T and K12-0792 represent a novel genus, for which the name Actinorhabdospora gen. nov. is proposed, with strain K12-0408 T (=NBRC 111897 T =TBRC 5327 T ) as the type strain of the type species, Actinorhabdospora filicis sp. nov.The family Micromonosporaceae was first described by Krasil'nikov (1938) and the description was amended by Koch et al. (1996), Stackebrandt et al. (1997) and Zhi et al. (2009) on the basis of chemotaxonomic data and 16S rRNA gene sequence analysis. During the characterization of actinomycetes isolated from rhizospheric soil of fern plants, strains K12-0408 T and K12-0792 were recovered. Phylogenetic analysis based on nearly complete 16S rRNA gene sequences indicated that the two strains were distinct from genera within the family Micromonosporaceae. Herein, we report the results of phenotypic, chemotaxonomic and genomic analyses of strains K12-0408 T and K12-0792, which suggested that the strains represented a novel species of a new genus.Strains K12-0408 T and K12-0792 were isolated from rhizosphere soil of ferns (pteridophyte plants) collected at Fussa and Hamura city, respectively, located in Tokyo prefecture, Japan. The CM-cellulose (CMC) medium used for the isolation procedure contained the following: 1.0 % CM-cellulose sodium salts, 0.1 % K 2 HPO 4 , 0.1 % L-asparagine monohydrate, 0.0001 % FeSO 4 Á7H 2 O, 0.0001 % MnCl 2 Á4H 2 O, 0.0001 % ZnSO 4 Á7H 2 O and 1.5 % agar (adjusted to pH 7.0±0.2 with 1 M NaOH). The medium was supplemented with 0.025 % nalidixic acid and 0.02 % Benlate (Dupont) to minimize and inhibit growth of unwanted bacteria and fungi, respectively. The pure cultures of strains K12-0408 T and K12-0792 were maintained on International Streptomyces Project (ISP) medium 2 (Shirling & Gottlieb, 1966) and in 20 % (v/v) glycerol suspension at À20 C. Biomass for taxonomic characterization was obtained from cultures grown at 27 C for 7 days in yeast extract-dextrose (YD) broth containing 1.0 % yeast extract and 1.0 % glucose.The morphological characteristics of strains K12-0408 T and K12-0792 ...

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“…Growth at different temperatures (4,10,15,20,25,28,32,35,37,40,45 and 50 °C) was determined on ISP 4 medium after incubation for 14 days. The pH range for growth (pH 3.0-11.0, at intervals of 1.0 pH unit) was tested in glucose-yeast extract broth (GY) [15] using the buffer system described by Zhao et al [16]. Tolerance of various NaCl concentrations (0-10 %, with an interval of 1 %, w/v) was tested in GY medium.…”

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confidence: 99%

Glycomyces albidus sp. nov., a novel actinobacterium isolated from rhizosphere soil of wheat (Triticum aestivum L.)

Qian

Duan

Lin

et al. 2020

International Journal of Systematic and Evolutionary Microbiology

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A novel actinobacterium, designated strain NEAU-7082T, was isolated from rhizosphere soil of wheat (Triticum aestivum L.) and characterized by using a polyphasic approach. Morphological and chemotaxonomic characteristics confirmed the affiliation of strain NEAU-7082T to the genus Glycomyces . 16S rRNA gene sequence analysis indicated that strain NEAU-7082T belonged to the genus Glycomyces and was closely related to Glycomyces mayteni JCM 16217T (99.0 % 16S rRNA gene sequence similarity), Glycomyces sambucus DSM 45047T (98.4 %), Glycomyces scopariae DSM 44968T (98.3 %), Glycomyces paridis DSM 102295T (98.1 %), Glycomyces artemisiae NBRC 109773T (98.0 %) and Glycomyces dulcitolivorans DSM 105121T (97.9 %). Phylogenetic analysis using the 16S rRNA gene sequence showed that the strain formed a stable clade with G. mayteni JCM 16217T and clustered with G. sambucus DSM 45047T, G. scopariae DSM 44968T, G. artemisiae NBRC 109773T and G. dulcitolivorans DSM 105121T in the genus Glycomyces . The cell wall contained meso-diaminopimelic acid and the whole-cell hydrolysates were glucose and xylose. The polar lipid profile consisted of diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), glycolipid (GL), phosphatidylinositol mannoside (PIM) and an unidentified lipid (UL). The menaquinones were MK-11(H4), MK-11 and MK-10. Major fatty acids were anteiso-C15 : 0, iso-C16 : 0 and anteiso-C17 : 0. These chemotaxonomic data substantiated the affiliation of strain NEAU-7082T to the genus Glycomyces . The DNA G+C content was 71.3 mol%. A combination of DNA–DNA hybridization results and some phenotypic characteristics demonstrated that strain NEAU-7082T could be distinguished from its closest relatives. Therefore, strain NEAU-7082T is considered to represent a novel species of the genus Glycomyces , for which the name Glycomyces albidus sp. nov. is proposed. The type strain is NEAU-7082T (=CCTCC AA 2019045T=JCM 33458T).

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Wangella harbinensis gen. nov., sp. nov., a new member of the family Micromonosporaceae

Cited by 100 publications

References 48 publications

Actinocorallia lasiicapitis sp. nov., an actinomycete isolated from the head of an ant (Lasius fuliginosus L.)

Actinocorallia lasiicapitis sp. nov., an actinomycete isolated from the head of an ant (Lasius fuliginosus L.)

Actinorhabdospora filicis gen. nov., sp. nov., a new member of the family Micromonosporaceae

Glycomyces albidus sp. nov., a novel actinobacterium isolated from rhizosphere soil of wheat (Triticum aestivum L.)

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