Walnut protein isolates attenuate particulate matter-induced lung and cardiac injury in mice and zebra fish

Zhang, Yuanyuan; Liu, Mingchuan; Fan, Ruiping; Zhou, Qianliu; Yang, Jin-ping; Yang, Shengjie; Wang, Chaojih; Kou, Junping

doi:10.1039/c9ra06002b

Cited by 7 publications

(4 citation statements)

References 46 publications

(55 reference statements)

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“…Such animal models of PM administration have been established and commonly used. 64,65 In this study, NCur treatment reduced mitochondrial ROS, mitochondrial fission, mitophagy, and inflammation in the aortas of PM-treated diabetic mice.…”

mentioning

confidence: 55%

Nanocurcumin Reduces High Glucose and Particulate Matter-Induced Endothelial Inflammation: Mitochondrial Function and Involvement of miR-221/222

Lai,

Lee,

Hsu

et al. 2023

IJN

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Purpose Particulate matter (PM) 2.5, harmful air pollutants, and diabetes are associated with high morbidity and mortality from cardiovascular disease (CVD). However, the molecular mechanisms underlying the combined effects of PM and diabetes on CVD remain unclear. Methods Endothelial cells (ECs) treated with high glucose (HG) and PM mimic hyperglycemia and air pollutant exposure in CVD. Endothelial inflammation was evaluated by Western blot and immunofluorescence of ICAM-1 expression and monocyte adhesion. The mechanisms underlying endothelial inflammation were elucidated through MitoSOX Red analysis, JC-1 staining, MitoTracker analysis, and Western blot analysis of mitochondrial fission-related, autophagy-related, and mitophagy-related proteins. Furthermore. nanocurcumin (NCur) pretreatment was used to test if it has a protective effect. Results ECs under co-exposure to HG and PM increased ICAM-1 expression and monocyte adhesion, whereas NCur pretreatment attenuated these changes and improved endothelial inflammation. PM exposure increased mitochondrial ROS levels, worsened mitochondrial membrane potential, promoted mitochondrial fission, induced mitophagy, and aggravated inflammation in HG-treated ECs, while NCur reversed these changes. Also, HG and PM-induced endothelial inflammation is through the JNK signaling pathway and miR-221/222 specifically targeting ICAM-1 and BNIP3. PM exposure also aggravated mitochondrial ROS levels, mitochondrial fission, mitophagy, and endothelial inflammation in STZ-induced hyperglycemic mice, whereas NCur attenuated these changes. Conclusion This study elucidated the mechanisms underlying HG and PM-induced endothelial inflammation in vitro and in vivo. HG and PM treatment increased mitochondrial ROS, mitochondrial fission, and mitophagy in ECs, whereas NCur reversed these conditions. In addition, miR-221/222 plays a role in the amelioration of endothelial inflammation through targeting Bnip3 and ICAM-1, and NCur pretreatment can modulate miR-221/222 levels. Therefore, NCur may be a promising approach to intervene in diabetes and air pollution-induced CVD.

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mentioning

confidence: 55%

Nanocurcumin Reduces High Glucose and Particulate Matter-Induced Endothelial Inflammation: Mitochondrial Function and Involvement of miR-221/222

Lai,

Lee,

Hsu

et al. 2023

IJN

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“…ARPE-19 cell viability decreased with PM 2.5 exposure in a dose-and time-dependent manner (Figure 1C), and was recovered by post-treatment with SFN for 6 h (Figure 1D and Supplementary Figure 1). DEX, a well-known anti-inflammatory drug (13,18), was used as a positive control. Furthermore, cellular LDH release significantly increased after 24 h exposure to PM 2.5 but decreased after treatment with 30 µM SFN (Figure 1E).…”

Section: Effects Of Sfn On Pm 25 -Induced Cell Death and Cytotoxicitymentioning

confidence: 99%

Sulforaphane Alleviates Particulate Matter-Induced Oxidative Stress in Human Retinal Pigment Epithelial Cells

et al. 2021

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Age-related macular degeneration (AMD) is a leading cause of blindness in the elderly, and oxidative damage to retinal pigment epithelial (RPE) cells plays a major role in the pathogenesis of AMD. Exposure to high levels of atmospheric particulate matter (PM) with an aerodynamic diameter of <2.5 μm (PM2.5) causes respiratory injury, primarily due to oxidative stress. Recently, a large community-based cohort study in the UK reported a positive correlation between PM2.5 exposure and AMD. Sulforaphane (SFN), a natural isothiocyanate found in cruciferous vegetables, has known antioxidant effects. However, the protective effects of SNF in the eye, especially in the context of AMD, have not been evaluated. In the present study, we evaluated the effect of SFN against PM2.5-induced toxicity in human RPE cells (ARPE-19) and elucidated the molecular mechanism of action. Exposure to PM2.5 decreased cell viability in ARPE-19 cells in a time- and dose-dependent manner, potentially due to elevated intracellular reactive oxygen species (ROS). SFN treatment increased ARPE-19 cell viability and decreased PM2.5-induced oxidative stress in a dose-dependent manner. PM2.5-induced downregulation of serum- and glucocorticoid-inducible kinase 1 (SGK1), a cell survival factor, was recovered by SFN. PM2.5 treatment decreased the enzymatic activities of the antioxidant enzymes including superoxide dismutase and catalase, which were restored by SFN treatment. Taken together, these findings suggest that SFN effectively alleviates PM2.5-induced oxidative damage in human ARPE-19 cells via its antioxidant effects, and that SFN can potentially be used as a therapeutic agent for AMD, particularly in cases related to PM2.5 exposure.

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“…5,11,12 Peptides have been reported to possess different biological properties, such as antioxidant, antihypertensive, enhance immunity, neuroprotective, enhancing memory, and anti-haze functions. 13 The effects of walnut peptides on boosting brain energy and intelligence have been widely explored. 11 Previous studies showed that peptides from enzymatically hydrolyzed walnut peptides could slow down the aging of mice and improve the learning and memory ability of mice in the water maze experiment by increasing acetylcholine receptors.…”

Section: Introductionmentioning

confidence: 99%

Walnut (Juglans regia L.) Oligopeptide Effects on Enhancing Memory, Cognition and Improving Sleep Quality in Teenagers and Elderly People in a Randomized Double-Blind Controlled Trial

Yeh¹,

Chao²,

Wen-fang³

et al. 2022

Natural Product Communications

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Walnut has been reported to have beneficial effects on improving cognitive performance. This randomized double-blind placebo-controlled clinical trial evaluates the clinical effectiveness and safety of walnut oligopeptide (WO) on memory enhancement, cognition, and sleep quality in teenagers and elderly people. Eighteen teenagers and 18 elderly people were, respectively, randomly allocated to placebo, low dosage (170 mg), and high dosage (340 mg) WO administration groups (n = 6 per group in each population). After 90 days of administration, the Wechsler Adult Intelligence Scale (WAIS) score was significantly increased and the global Pittsburgh Sleep Quality Index (PSQI) score was significantly decreased in the WO administration group. In addition, the average scores for test subjects of Chinese, Mathematics, and English examinations were significantly increased from the baseline for the teenagers in the WO administration group. Our results support the claim that WO has the potential to become a new option for nutritional intervention, to enhance the memory, cognitive ability, and sleep quality of teenagers and elderly people. This study was approved by the Institutional Review Board of the Shanghai Nutrition Society and registered at the Chinese Clinical Trial Registry ( http://www.chictr.org.cn ) with an ID number of ChiCTR1900028160.

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Walnut protein isolates attenuate particulate matter-induced lung and cardiac injury in mice and zebra fish

Abstract: This study supports new prospects for WPI development and shows WPIs may be potential candidates for healthy products.

Cited by 7 publications

References 46 publications

Nanocurcumin Reduces High Glucose and Particulate Matter-Induced Endothelial Inflammation: Mitochondrial Function and Involvement of miR-221/222

Nanocurcumin Reduces High Glucose and Particulate Matter-Induced Endothelial Inflammation: Mitochondrial Function and Involvement of miR-221/222

Sulforaphane Alleviates Particulate Matter-Induced Oxidative Stress in Human Retinal Pigment Epithelial Cells

Walnut (Juglans regia L.) Oligopeptide Effects on Enhancing Memory, Cognition and Improving Sleep Quality in Teenagers and Elderly People in a Randomized Double-Blind Controlled Trial

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