Walking through trabecular meshwork biology: Toward engineering design of outflow physiology

Dautriche, Cula N.; Xie, Yubing; Sharfstein, Susan T.

doi:10.1016/j.biotechadv.2014.04.012

Cited by 38 publications

(53 citation statements)

References 136 publications

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“…The small segment between lens and iris serves as inlet for AH flow. The width of TM at anterior and posterior portion is 70 and 100 μm, respectively . Schlemm's canal of average width 125 μm is modelled at the top of TM .…”

Section: Mathematical Model and Numerical Proceduresmentioning

confidence: 99%

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A novel patient‐oriented numerical procedure for glaucoma drainage devices

Mauro

Massarotti

Mohamed

et al. 2018

Numer Methods Biomed Eng

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The present work analyses the performance of four glaucoma drainage devices, by means of a novel patient-oriented numerical procedure. The procedure is based on the three-dimensional geometry reconstruction from the stacks of tomographic images of a human eye, at different angles, on meshing and on thermo-fluid dynamics modelling activities, carried out on the reconstructed computational domain. The current three-dimensional eye model considers anterior chamber (AC), trabecular meshwork, Schlemm's canal, and collector channels, making use of generalised porous medium approach for modelling ocular porous tissue and cavities. The intraocular pressure (IOP) management inside AC of human eye is analysed, by comparing the results obtained for four drainage devices implanted in a human eye for glaucoma treatment, ie, ExPRESS shunt, iStent inject, SOLX gold micro shunt, and the novel silicon shunt device. The numerical results allow predicting the effects of the installation of these implants on human eyes, in terms of IOP decrease, aqueous humour velocity, pressure, friction coefficient, and local Nusselt number, pointing out the clear distinction between pre-operative and post-operative eye conditions for different glaucoma surgical techniques.

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Section: Mathematical Model and Numerical Proceduresmentioning

confidence: 99%

“…The increased IOP leads to optic nerve cupping, resulting in loss of optic nerve cell, retinal ganglion. 2 After a chronic deterioration of optic nerve cells, eye vision reduces to a stage that is not possible to recover. Therefore, glaucoma is an irreversible eye disease, and glaucoma post treatment after loss of vision is futile.…”

Section: Introductionmentioning

confidence: 99%

A novel patient‐oriented numerical procedure for glaucoma drainage devices

Mauro

Massarotti

Mohamed

et al. 2018

Numer Methods Biomed Eng

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“…Contrary to TM cells that are fairly easy to culture, SC cells are notoriously hard to obtain in culture (Dautriche et al, 2014). The method most often used for generating such primary cultures involves the placement of a suture in the SC and lengthy incubations (Stamer et al, 1998).…”

Section: Ex-vivo Model Systemsmentioning

confidence: 99%

“…Culturing TM and SC cells allows for the observation and study of structural characteristics, biological properties, as well as the growth patterns of the cells (Dautriche et al, 2014). Gene and protein expression after exposure to steroids have been reported (for examples see (Bollinger et al, 2011, 2012; Zhao et al, 2004)) and contribute to our current understanding of some of the processes that lead to steroid-induced IOP elevation.…”

Section: Ex-vivo Model Systemsmentioning

confidence: 99%

Model systems for the study of steroid-induced IOP elevation

Rybkin

Gerometta

Fridman

et al. 2017

Experimental Eye Research

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Steroid-induced IOP elevation affects a significant number of patients. It results from a decrease in outflow facility of the aqueous humor. To understand the pathophysiology of this condition a number of model systems have been created. These include ex-vivo cell and organ cultures as well as in-vivo animal models in organisms ranging from rodents to primates. These model systems can be used to investigate specific aspects of steroid-induced IOP elevation. This brief review summarizes the strengths and limitations of the various model systems and provides examples of where these systems have been successfully used to advance our understanding of steroid-induced IOP elevation.

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“…Traditional two dimensional cell culture studies have produced useful information about the physiology of TM cells, but they fail to capture the complexity of the three-dimensional environment that native TM cells inhabit (Dautriche et al, 2014;Jacobson et al, 2001;Llobet et al, 2003;McKee et al, 2011;Obazawa et al, 2004;Polansky et al, 1997;Tamm et al, 1999;Wood et al, 2011;Yue et al, 1984). It has been shown that the function and morphology of TM cells is dependent on the topographical environment to which they are exposed (Kim et al, 2011;McKee et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Collagen and collagen‐chondroitin sulfate scaffolds with uniaxially aligned pores for the biomimetic, three dimensional culture of trabecular meshwork cells

Osmond

Bernier

Pantcheva

et al. 2016

Biotech & Bioengineering

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Glaucoma is a disease in which damage to the optic nerve leads to progressive, irreversible vision loss. The intraocular pressure (IOP) is the only modifiable risk factor for glaucoma and its lowering is considered a useful strategy for preventing or slowing down the progression of glaucomatous neuropathy. Elevated intraocular pressure associated with glaucoma is due to increased aqueous humor outflow resistance, primarily through the trabecular meshwork (TM) of the eye. Current in vitro models of the trabecular meshwork are oversimplified and do not capture the organized and complex three-dimensional nature of this tissue that consists primarily of collagen and glycoasaminoglycans. In this work, collagen and collagen-chondroitin sulfate (CS) scaffolds were fabricated via unidirectional freezing and lyophilization to induce the formation of aligned pores. Scaffolds were characterized by scanning electron microscopy, dynamic mechanical analysis, and a chondroitin sulfate quantification assay. Scaffold characterization confirmed the formation of aligned pores, and also that the CS was leaching out of the scaffolds over time. Primary porcine trabecular meshwork (TM) cells were seeded onto the surface of scaffolds and their gene expression, proliferation, viability, migration into the scaffolds, and morphology were examined. The TM cells were viable and proliferated 2 weeks after seeding. The cells migrated down into the internal scaffold structure and their morphology reflected the topography and alignment of the scaffold structure. This work is a promising step toward the development of a three dimensional in vitro model of the TM that can be used for testing of glaucoma pharmacological agents in future experimentation and to better our understanding of the trabecular meshwork and its complex physiology. Biotechnol. Bioeng. 2017;114: 915-923. © 2016 Wiley Periodicals, Inc.

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Walking through trabecular meshwork biology: Toward engineering design of outflow physiology

Cited by 38 publications

References 136 publications

A novel patient‐oriented numerical procedure for glaucoma drainage devices

A novel patient‐oriented numerical procedure for glaucoma drainage devices

Model systems for the study of steroid-induced IOP elevation

Collagen and collagen‐chondroitin sulfate scaffolds with uniaxially aligned pores for the biomimetic, three dimensional culture of trabecular meshwork cells

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