Walking dead: Permeabilization of heat-treated Geobacillus stearothermophilus ATCC 12980 spores under growth-preventing conditions

Mtimet, Narjes; Trunet, Clément; Mathot, Anne-Gabrielle; Venaille, Laurent; Leguérinel, Ivan; Coroller, Louis; Couvert, Olivier

doi:10.1016/j.fm.2016.12.013

Cited by 6 publications

(3 citation statements)

References 39 publications

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“…(2005); Mtimet et al . (2017); Reis et al . (2005) and therefore has the potential to underestimate the microbiological population being examined.…”

Section: Limitations Of Current Techniques In Sterilization Microbiologymentioning

confidence: 99%

“…Furthermore, culture-based techniques assume that one colony forming unit has emanated from a single cell, which may not be the case as a colony can arise from one or more cells. This limitation has been highlighted by many authors such as Ou et al (2017); Laflamme et al (2005); Mtimet et al (2017); Reis et al (2005) and therefore has the potential to underestimate the microbiological population being examined. Furthermore, as identified by both Ou et al (2017) and Laflamme et al (2005), culturability is inherently dependent on culture conditions.…”

Section: Binary Growth Of Micro-organism and Culture Conditions Post-treatmentmentioning

confidence: 99%

See 1 more Smart Citation

Opportunities for the application of real‐time bacterial cell analysis using flow cytometry for the advancement of sterilization microbiology

McEvoy¹,

Lynch

Rowan

2020

J Appl Microbiol

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Medical devices provide critical care and diagnostic applications through patient contact. Sterility assurance level (SAL) may be defined as the probability of a single viable microorganism occurring on an item after a sterilization process. Sterilization microbiology often relies upon using an overkill validation method where a 12-log reduction in recalcitrant bacterial endospore population occurs during the process that exploits conventional laboratory-based culture media for enumeration. This timely review explores key assumptions underpinning use of conventional culture-based methods in sterilization microbiology. Consideration is given to how such methods may limit the ability to fully appreciate the inactivation kinetics of a sterilization process such as vaporized hydrogen peroxide (VH2O2) sterilization, and consequently design efficient sterilization processes. Specific use of the real-time flow cytometry (FCM) is described by way of elucidating the practical relevance of these limitation factors with implications and opportunities for the sterilization industry discussed. Application of FCM to address these culture-based limitation factors will inform real-time kinetic inactivation modelling and unlock potential to embrace emerging opportunities for pharma, medical device and sterilization industries including potentially disruptive applications that may involve reduced usage of sterilant.

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“…(2005); Mtimet et al . (2017); Reis et al . (2005) and therefore has the potential to underestimate the microbiological population being examined.…”

Section: Limitations Of Current Techniques In Sterilization Microbiologymentioning

confidence: 99%

Section: Binary Growth Of Micro-organism and Culture Conditions Post-treatmentmentioning

confidence: 99%

Opportunities for the application of real‐time bacterial cell analysis using flow cytometry for the advancement of sterilization microbiology

McEvoy¹,

Lynch

Rowan

2020

J Appl Microbiol

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“…Notably, spores killed by many of the agents listed above can often still germinate, albeit sometimes slower than untreated spores. This is true for spores killed by radiation, dry and wet heat and many chemicals (Mtimet et al 2017 ; Rao et al 2016 ; Russell and Loosemore 1964 ; Setlow et al 2013 ). Agents which target proteins involved in germination include wet heat and some chemicals.…”

Section: Spore Killingmentioning

confidence: 99%

What’s new and notable in bacterial spore killing!

Setlow

Christie

2021

World J Microbiol Biotechnol

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Spores of many species of the orders Bacillales and Clostridiales can be vectors for food spoilage, human diseases and intoxications, and biological warfare. Many agents are used for spore killing, including moist heat in an autoclave, dry heat at elevated temperatures, UV radiation at 254 and more recently 222 and 400 nm, ionizing radiation of various types, high hydrostatic pressures and a host of chemical decontaminants. An alternative strategy is to trigger spore germination, as germinated spores are much easier to kill than the highly resistant dormant spores—the so called “germinate to eradicate” strategy. Factors important to consider in choosing methods for spore killing include the: (1) cost; (2) killing efficacy and kinetics; (3) ability to decontaminate large areas in buildings or outside; and (4) compatibility of killing regimens with the: (i) presence of people; (ii) food quality; (iii) presence of significant amounts of organic matter; and (iv) minimal damage to equipment in the decontamination zone. This review will summarize research on spore killing and point out some common flaws which can make results from spore killing research questionable.

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Evaluation of the additive effects of volatile fatty acids and moderate heat treatment for enhancing the inactivation of vegetative cells and spores of Clostridium perfringens by flow cytometry

Delmon,

Ouk,

Casellas

et al. 2023

Anaerobe

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Walking dead: Permeabilization of heat-treated Geobacillus stearothermophilus ATCC 12980 spores under growth-preventing conditions

Cited by 6 publications

References 39 publications

Opportunities for the application of real‐time bacterial cell analysis using flow cytometry for the advancement of sterilization microbiology

Opportunities for the application of real‐time bacterial cell analysis using flow cytometry for the advancement of sterilization microbiology

What’s new and notable in bacterial spore killing!

Evaluation of the additive effects of volatile fatty acids and moderate heat treatment for enhancing the inactivation of vegetative cells and spores of Clostridium perfringens by flow cytometry

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