WAKTU REGENERASI BAKTERI Vibrio cholerae PADA MEDIUM APW

Sariadji, Kambang; Wati, Melati; Syamsidar, Syamsidar; Novi, A.; Sundari, Sundari; Khariri, Khariri; Sunarno, Sunarno

doi:10.22435/bpk.v43i1.3966.35-40

Cited by 2 publications

(3 citation statements)

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“…In addition to the above, multiplex PCR (mPCR) assays, based on conventional or real-time systems, have been designed to combine tox gene detection with identification of corynebacterial species [79][80][81][82][83][84]. The species-specific conventional mPCR proposed by Pimenta et al [80] combined the detection of toxA and toxB with C. diphtheriae identification using primers targeting dtxR, the product of which acts as a global regulator of metabolism in both toxigenic and non-toxigenic strains, including the regulation of tox expression [80].…”

Section: Polymerase Chain Reactionmentioning

confidence: 99%

“…The species-specific conventional mPCR proposed by Pimenta et al [80] combined the detection of toxA and toxB with C. diphtheriae identification using primers targeting dtxR, the product of which acts as a global regulator of metabolism in both toxigenic and non-toxigenic strains, including the regulation of tox expression [80]. Another conventional mPCR based on the amplification of the tox and dtxR genes was described by Sunarno et al [84], which, in addition to tox detection and differentiation of three potentially toxigenic corynebacterial species (C. diphtheriae, C. ulcerans, and C. pseudotuberculosis), was also used to predict some NTTB type strains (deletions at position 25 or 55 of the tox gene) [84]. The conventional pentaplex PCR assay proposed by Torrez et al [81], in addition to tox-specific primers (a region between fragments A and B), contains primers for detection of C. diphtheriae, C. ulcerans, and C. pseudotuberculosis.…”

Section: Polymerase Chain Reactionmentioning

confidence: 99%

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Challenges of Diphtheria Toxin Detection

Prygiel,

Mosiej,

Polak

et al. 2024

Toxins

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Diphtheria toxin (DT) is the main virulence factor of Corynebacterium diphtheriae, C. ulcerans and C. pseudotuberculosis. Moreover, new Corynebacterium species with the potential to produce diphtheria toxin have also been described. Therefore, the detection of the toxin is the most important test in the microbiological diagnosis of diphtheria and other corynebacteria infections. Since the first demonstration in 1888 that DT is a major virulence factor of C. diphtheriae, responsible for the systemic manifestation of the disease, various methods for DT detection have been developed, but the diagnostic usefulness of most of them has not been confirmed on a sufficiently large group of samples. Despite substantial progress in the science and diagnostics of infectious diseases, the Elek test is still the basic recommended diagnostic test for DT detection. The challenge here is the poor availability of an antitoxin and declining experience even in reference laboratories due to the low prevalence of diphtheria in developed countries. However, recent and very promising assays have been developed with the potential for use as rapid point-of-care testing (POCT), such as ICS and LFIA for toxin detection, LAMP for tox gene detection, and biosensors for both.

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Section: Polymerase Chain Reactionmentioning

confidence: 99%

Section: Polymerase Chain Reactionmentioning

confidence: 99%

Challenges of Diphtheria Toxin Detection

Prygiel,

Mosiej,

Polak

et al. 2024

Toxins

View full text Add to dashboard Cite

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“…Menurut Wahyuningsih dan Zulaikha (2018) bahwa kandungan NB terdiri dari sumber karbon berupa beef ekstrak dan pepton sebagai sumber nitrogen yang berperan sebagai kebutuhan nutrisi bakteri. Apabila dalam kondisi lingkungan dan nutrisi makanan yang cocok, maka bakteri dapat terus berkembang dan memperbanyak diri dengan cara membelah diri (Sariadji et al, 2013).…”

Section: Isolasi Dan Identifikasi Bakteriunclassified

Isolasi dan Identifikasi Bakteri Gram Positif Staphylococcus aureus dan Micrococcus pada Peternakan Sapi yang Terindikasi Mastitis

Ayuti

Hidayati²,

Admi³

et al. 2023

JPI

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Mastitis menjadi salah satu penyakit yang menyebabkan penurunan produksi ternak dan sulit disembuhkan dengan menggunakan antibiotik dan dapat menimbulkan residu, serta memicu resistensi antibiotik. Penelitian ini bertujuan untuk mengetahui bakteri Gram positif yang dapat menjadi penyebab mastitis pada sapi. Penelitian menggunakan sampel yang diambil dari peternakan rakyat dengan cara swab ambing ternak. Isolasi bakteri dilakukan dengan cara penanaman pada media Mannitol Salt Agar (MSA) dan melakukan Identifikasi dengan pewarnaan Gram, Uji Katalase dan Uji biokimia (maltosa dan laktosa). Data dianalisis menggunakan analisis deskriptif yaitu jenis-jenis bakteri sebagai agen penyebab mastitis. Hasil penelitian yang didapatkan sebagai bakteri penyebab mastitis spesies Staphylococcus aureus dan Micrococcus sp. yang tergolong pada bakteri Gram positif. Dapat disimpulkan bahwa pada sapi terindikasi mastitis terdapat bakteri Gram positif, hal tersebut secara morfologi diidentifikasi yaitu Staphylococcus aureus dan Micrococcus.

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WAKTU REGENERASI BAKTERI Vibrio cholerae PADA MEDIUM APW

Cited by 2 publications

References 0 publications

Challenges of Diphtheria Toxin Detection

Challenges of Diphtheria Toxin Detection

Isolasi dan Identifikasi Bakteri Gram Positif Staphylococcus aureus dan Micrococcus pada Peternakan Sapi yang Terindikasi Mastitis

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