Waktu Inkubasi pada Derajat Distilasi Kitosan Enzim dan Efektifitas Penghambatannya terhadap Penyakit Antraknosa

Abstract

The use of chitosan as a coating agent of harvested fruits is an alternative method in controlling anthracnose disease (Colletotrichum sp.). This study aimed to obtain an optimal enzymatic chitosan (EC) that hydrolyzed using chitinase from Burkholderia cepacia isolate E76. The optimal incubation condition to produce EC was 2 h with the yield of 3.52 ± 0.38 g. The degree of deacetylation (DD) chitosan and EC was 66.91% and 80.91%, respectively. Based on in vitro assays, EC 2% was the most effective in inhibiting the growth of Colletotrichum sp. (94.22%) than chitosan, while the highest inhibition for chitosan 3% was 55.26%. Moreover, the EC 2% showed the highest inhibition of spore germination (74.12%). The in vivo assay revealed that EC 2% showed the highest inhibition on the fungal growth (88.88%), compared to the other concentrations. On the other hand, the EC 2% and 3% gave similar results on inhibition of Colletotrichum sp.of chili (55.55%).

Control of Anthracnose Disease (Colletotrichum gloeosporioides) Using Nano Chitosan Hydrolyzed by Chitinase Derived from Burkholderia cepacia Isolate E76

“…It was also reported that the hydrolyzed chitosan using chitinase produced by B. cepacia isolate E76 at concentration of 2% showed as the most effective treatment in inhibiting the growth of Colletotrichum sp. (94.22%) than that of non-hydrolyzed chitosan (Suryadi et al 2016).…”

“…The HMW chitosan was more sensitive to depolymerization (Mao et al 2004). In our previous study, a LMW of the depolymerized HMW chitosan with a high degree polymerization was successfully done using chitinase extracted from B. cepacia isolate E76 (Suryadi et al 2016). This depolymerization was based on the enzymatic hydrolysis process by breaking the chitosan bonds at 1,4-β-glycosidic site (2009).…”

Aktivitas Antifungi Formula Kitosan-Tripolifosfat Terhadap Infeksi Colletotrichum spp. Pada Cabai

“…Hasil penelitian terdahulu dilaporkan bahwa aktivitas kitinase yang diperoleh dari isolat B.cepacia E.76 cukup tinggi karena bakteri kitinolitik tersebut menghasilkan enzim yang berfungsi untuk mendegradasi kitosan menjadi K-BMR, dan selanjutnya menghasilkan formulasi K-TPP yang lebih efektif dalam menghambat pertumbuhan Colletotrichum spp. (Suryadi et al, 2016;Suryadi et al, 2017).…”

“…sebesar 94.22%, serta menekan perkecambahan spora sebesar 74.12%. Uji in vivo K-BMR yang dilakukan terhadap C. gloeosporioides pada pepaya menunjukkan penghambatan terbaik sebesar 88.88% pada konsentrasi 2%, sedangkan pada cabai menunjukkan penghambatan terbaik sebesar 55.55% pada konsentrasi 2% dan 3% (Suryadi, et al, 2016). Hasil penelitian lain juga menunjukkan bahwa pada uji in vitro, kisaran konsentrasi kitosan 0.75 -1% mampu menghambat pertumbuhan Colletotrichum spp.…”

Aplikasi Edible Coating Tepung Tapioka Dengan Oleoresin Daun Kemangi untuk Memperpanjang Umur Simpan Buah Jambu Air Cincalo (Syzygium samarangense [Blume] Merril & L.M. Perry)

“…(Pangestika 2015;Purwandari 2015) dan Colletotrichum gloeosporioides (Udayanga et al 2013) yang menyebabkan busuk pada dasar buah. Menurut Suryadi et al (2016), penggunaan kitosan enzimatik sebagai edible coating efektif menghambat pertumbuhan patogen Colletotrichum sp. penyebab penyakit antraknosa dengan konsentrasi 2% pada pepaya dan konsentrasi 3% pada cabai.…”