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Ahmed, Aneesh; Ezziyyani, Mostafa; Sánchez, Consuelo Pérez; Castillo, María Emilia Candela

doi:10.1023/a:1024734216814

Cited by 106 publications

(13 citation statements)

References 10 publications

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“…This reduction reached 44.44% with G. virens, 37.03% with T. harzianum and 14.8% with T. viride, relative to R. solani-inoculated and untreated control. Our results are consistent, in part, with those of Sid Ahmed et al [15] who demonstrated that seed treatment and soaking pepper roots with T. harzianum led to 44% decrease in root rot caused by Phytophthora capsici and to 38% in that induced by R. solani.…”

Section: Discussionsupporting

confidence: 93%

“…Similarly, Mojica-Marín et al [20], working on pepper diseases, showed that B. thuringensis is effective in controlling R. solani in vitro. In addition, seed treatment and root dipping in B. subtilis and B. licheniformis cell suspensions reduced the severity of root rot caused by Phytophthora by 55% and 50% and that caused by R. solani by 44% and 55%, respectively [15].…”

Section: Discussionmentioning

confidence: 98%

“…These species-controlled R. solani by diverse mechanisms [13][14][15][16][17]. In fact, these species act as competitive hyperparasites, producing antifungal metabolites, whether volatile or not and hydrolytic enzymes that cause structural changes at cell level, such as vacuolization, granulation, cytoplasm disintegration and cell lysis, which have been observed in organisms with which they interact.…”

Section: Introductionmentioning

confidence: 99%

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Rhizoctonia Root Rot of Pepper (Capsicum annuum): Comparative Pathogenicity of Causal Agent and Biocontrol Attempt using Fungal and Bacterial Agents

Mannai¹,

Jabnoun‐Khiareddine²,

Nasraoui³

et al. 2018

J Plant Pathol Microbiol

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Rhizoctonia root rot of pepper (Capsicum annuum L.) is becoming serious in Tunisia. Comparative pathogenicity tests performed for Rhizoctonia solani isolates recovered from pepper and potato showed that Rhiz.7 and Rhiz.4 were the most aggressive. They reduced by 53.5%-91.4% the aerial part fresh weight of inoculated cv. Baklouti plants relative to control. Rhiz.7 decreased by 81%-88% the root fresh weight on cvs. Beldi and Baklouti. Various fungal and bacterial agents were tested against R. solani. Dual culture trials showed that Trichoderma harzianum, T. viride and Glicladium virens grew and sporulated profusely over R. solani colonies and altered its hyphae. Pseudomonas huttiensis 69, P. aureofaciens 31 and Burkholderia glathei 35 reduced pathogen growth by 9.71-12.87%. These bio-agents were tested for their effects on rhizoctonia root rot disease and pepper growth. On cv. Beldi, pre-emergence damping-off, noted after 15 days, was suppressed by 55 (for G. virens), 45 (for T. viride) and 50% (for T. harzianum). This inhibition reached 57.14% using Bacillus pumilus 420 and P. putida 227. Tested on pepper cv. Altar, all tested fungi decreased by 40% post-emergence damping-off, and significantly increased the plant height of R. solani-inoculated and treated plants by 21.13 (for T. viride) to 36.34% (for T. harzianum) relative to control. P. aureofaciens 314 and P. putida 227 completely suppressed R. solani post-emergence expression. Treatments with P. aureofaciens 314, P. aureofaciens 31, Bacillus pumilus 420, P. fluorescens Pf and P. putida 227 induced a significant increase in their height compared to control. An improvement of the aerial part fresh weight by 54.54, 48.09 and 47.74%, as compared to control, was induced by P. aureofaciens 314, B. glathei 35 and P. huttiensis 69, respectively. Indeed, strains of B. thuringensis were found to be efficient for the biocontrol of R. solani of chili pepper based on in vitro assays [20]. Moreover, B. cepacia was shown able to reduce the severity of Rhizoctonia diseases associated to pepper and tomato [19]. Antibiosis seems to be their principal mode of action [21].Pseudomonas species were shown capable of markedly inhibiting the growth of R. solani in vitro and in vivo. Indeed, tomato plants were also highly protected against R. solani infestations using this bacterium suspended in water [19]. Moreover, fluorescent Pseudomonas species were found to induce systemic resistance in plants as a result of root colonization [18].Recently, several rhizobacterial isolates and mainly B. thuringiensis B2 (KU158884), B. subtilis B10 (KT921327) and Enterobacter cloacae B16 (KT921429) were found to be efficient for the suppression of R. solani radial growth and disease severity and for the enhancement of tomato growth [10].In Tunisia, R. solani is still being a destructive pathogen of pepper and investigations for its biocontrol are lacking. Therefore, the objectives of the current study were: (i) to evaluate the aggressiveness of different R. solani isolates involved in dam...

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Rhizoctonia Root Rot of Pepper (Capsicum annuum): Comparative Pathogenicity of Causal Agent and Biocontrol Attempt using Fungal and Bacterial Agents

Mannai¹,

Jabnoun‐Khiareddine²,

Nasraoui³

et al. 2018

J Plant Pathol Microbiol

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“…The relevant data reported by the chitinase inhibition against Aspergillus niger (7 mm), Aspergillus flavus (5 mm) (MUHAMAD, 2009). The present result is supported by the previous literature as the chitinolytic bacteria are active against the fungal pathogens (SID AHMED et al, 2003;DE BOER et al,1999). Recently, chitinases have been receiving attention because of their possible application for biological control and gaining importance for their biotechnological applications as it is biodegradable with various applications in the environmental, agricultural, food technology and cosmetics.…”

Section: Antifungal Activitysupporting

confidence: 89%

Production and partial purification of antifungal chitinase from Bacillus cereus VITSD3

Devi¹,

Srinivasan²,

Archana³

et al. 2015

Biosci. J.

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ABSTRACT:The current work was designed to isolate and characterize chitin degrading bacteria. Among the 55 bacterial colonies isolated from 7 different soil samples, 4 isolates were capable of degrading chitinase, among which one strain VITSD3 was found to be potent. Based on the morphological, biochemical and molecular characterization of VITSD3 the isolate was confirmed as Bacillus cereus (Genbank accession number: KC961638), designated as Bacillus cereus VITSD3. The crude enzyme had a total activity of 220 U, precipitated with 44.8 U and 22.5 U for dialysed sample. The hydrolysed product NAG (N-Acetyl Glucosamine) from chitin was analysed by high-pressure liquid chromatography (HPLC).The molecular weight of the chitinase was determined using SDS PAGE and found to be 55 kDa. The partially purified chitinase produced from Bacillus cereus VITSD3 showed antifungal activity against Aspergillus fumigatus (18 mm), Aspergillus niger (6 mm) and Aspergillus flavus (15 mm). Hence the investigation suggests a potential benefit of partially purified chitinase extracted from Bacillus cereus VITSD3 which will serve as an excellent antifungal potential with therapeutic use.

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“…The design of an effective bacterial biocontrol product is challenging, since a thorough understanding of target, environment, mode of action, and delivery system is needed (O'Brien, 2017). Biocontrol bacteria are often packaged together with chitin, which has long been thought to prime their anti-fungal behaviors (Sid Ahmed et al, 2003;Kokalis-Burelle et al, 2006;Yandigeri et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Exploring the Potential for Fungal Antagonism and Cell Wall Attack by Bacillus subtilis natto

et al. 2020

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To develop more ecologically sustainable agricultural practices requires that we reduce our reliance on synthetic chemical pesticides for crop protection. This will likely involve optimized biocontrol approaches -the use of beneficial soil microbes to attack potential plant pathogens to protect plants from diseases. Many bacterial species, including strains of Bacillus subtilis, have been explored for their biocontrol properties, as they can control the growth of harmful fungi, often by disrupting the fungal cell wall. A strain that is not often considered for this particular application is Bacillus subtilis natto, primarily known for fermenting soybeans via cell wall degradation in the Japanese probiotic dish "natto." Because deconstruction of the fungal cell wall is considered an important biocontrol trait, we were motivated to explore the possible anti-fungal properties of the B. subtilis natto strain. We show that B. subtilis natto can use complex fungal material as a carbon source for growth, and can effectively deconstruct fungal cell walls. We found degradation of fungal cell wall proteins, and showed that growth on a mix of peptides was very strong. We also found that intact fungal cell walls can induce the secretion of chitinases and proteases. Surprisingly, we could show that chitin, the bulk component of the fungal cell wall, does not permit successful growth of the natto strain or induce the secretion of chitinolytic enzymes, although these were produced during exposure to proteins or to complex fungal material. We have further shown that protease secretion is likely a constitutively enabled mechanism for nutrient scavenging by B. subtilis natto, as well as a potent tool for the degradation of fungal cell walls. Overall, our data highlight B. subtilis natto as a promising candidate for biocontrol products, with relevant behaviors that can be optimized by altering growth conditions. Whereas it is common for bacterial biocontrol products to be supplied with chitin or chitosan as a priming polysaccharide, our data indicate that this is not a useful approach with this particular bacterium, which should instead be supplied with either glucose or attenuated fungal material.

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Cited by 106 publications

References 10 publications

Rhizoctonia Root Rot of Pepper (Capsicum annuum): Comparative Pathogenicity of Causal Agent and Biocontrol Attempt using Fungal and Bacterial Agents

Rhizoctonia Root Rot of Pepper (Capsicum annuum): Comparative Pathogenicity of Causal Agent and Biocontrol Attempt using Fungal and Bacterial Agents

Production and partial purification of antifungal chitinase from Bacillus cereus VITSD3

Exploring the Potential for Fungal Antagonism and Cell Wall Attack by Bacillus subtilis natto

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