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Tsunemori, Hideyuki; Araki, Kosuke; Uezu, Kazuya; Goto, Masahiro; Furusaki, Shintaro

doi:10.1023/a:1021541803571

Cited by 23 publications

(6 citation statements)

References 12 publications

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“…Molecularly imprinted polymers (MIPs) have the ability to recognize biological compounds like proteins [1,2,3,4], amino acids [5,6,7], peptides [8,9,10,11] or nucleotides [12], and chemicals such as pollutants [13], drugs and food additives [14,15,16]. Moreover, they can be applied in separation and purification processes [17], chemical sensors [18], catalysis [19] and drug delivery [20].…”

Section: Introductionmentioning

confidence: 99%

Imprinting Technology in Electrochemical Biomimetic Sensors

Frasco

Truta

Sales

et al. 2017

Sensors

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Biosensors are a promising tool offering the possibility of low cost and fast analytical screening in point-of-care diagnostics and for on-site detection in the field. Most biosensors in routine use ensure their selectivity/specificity by including natural receptors as biorecognition element. These materials are however too expensive and hard to obtain for every biochemical molecule of interest in environmental and clinical practice. Molecularly imprinted polymers have emerged through time as an alternative to natural antibodies in biosensors. In theory, these materials are stable and robust, presenting much higher capacity to resist to harsher conditions of pH, temperature, pressure or organic solvents. In addition, these synthetic materials are much cheaper than their natural counterparts while offering equivalent affinity and sensitivity in the molecular recognition of the target analyte. Imprinting technology and biosensors have met quite recently, relying mostly on electrochemical detection and enabling a direct reading of different analytes, while promoting significant advances in various fields of use. Thus, this review encompasses such developments and describes a general overview for building promising biomimetic materials as biorecognition elements in electrochemical sensors. It includes different molecular imprinting strategies such as the choice of polymer material, imprinting methodology and assembly on the transduction platform. Their interface with the most recent nanostructured supports acting as standard conductive materials within electrochemical biomimetic sensors is pointed out.

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Section: Introductionmentioning

confidence: 99%

Imprinting Technology in Electrochemical Biomimetic Sensors

Frasco

Truta

Sales

et al. 2017

Sensors

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“…To date, with the conventional monolithic approach, the technique has been successfully applied to peptides, 2 metal ions, 3 and nucleotides. 4 However, the degree of success associated with the imprinting of macromolecules like proteins and polysaccharides has been limited. This is primarily due to the difficulty of template removal and the restricted accessibility of the embedded binding sites to these large molecules.…”

Section: Introductionmentioning

confidence: 99%

The Effect of Protein Structural Conformation on Nanoparticle Molecular Imprinting of Ribonuclease A Using Miniemulsion Polymerization

Tan

Tong

2007

Langmuir

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One of the major difficulties faced in the molecular imprinting of proteins is the inherently fragile and flexible nature of the protein template which makes it incompatible with most polymerization systems. Miniemulsion polymerization is a possible approach for preparing molecularly imprinted nanoparticles, and in this study, the method of initiation, the high-shear homogenization, and the surfactant used for the polymerization reaction had been considered as possible factors that can denature the template protein, ribonuclease A (RNase A). The conformation of the protein in a miniemulsion was studied using circular dichroism (CD). It was found that redox initiation was more suitable for protein imprinting and that the addition of poly(vinyl alcohol) (PVA) as a co-surfactant had proved to be effective in preserving the template protein structural integrity. On the basis of the results of the study, polymeric nanoparticles imprinted with RNase A were prepared via miniemulsion polymerization using methyl methacrylate (MMA) and ethylene glycol dimethacrylate (EGDMA) as the functional and cross-linker monomers, respectively, with the conditions of the polymerization system optimized to best preserve the integrity of the protein template. In the subsequent investigation for the recognition properties of the prepared nanoparticles through batch and competitive rebinding tests, the imprinted nanoparticles prepared through the conventional (nonoptimized) miniemulsion polymerization lacked the target specificity as displayed by those prepared under the optimized conditions. This illustrated the importance of protein structural integrity in protein imprinting.

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“…A number of reports focused on the synthesis of polymers via imprinting applied for aminoacids [20], peptides [21], carbohydrates [22], nucleotides [23], hormones [24], drugs [25], toxins [26], pesticides [27]. …”

Section: Use Of Molecularly Imprinted Polymers In Affinity Recognimentioning

confidence: 99%

Imprinting of Microorganisms for Biosensor Applications

İdil

Mattìasson

2017

Sensors

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There is a growing need for selective recognition of microorganisms in complex samples due to the rapidly emerging importance of detecting them in various matrices. Most of the conventional methods used to identify microorganisms are time-consuming, laborious and expensive. In recent years, many efforts have been put forth to develop alternative methods for the detection of microorganisms. These methods include use of various components such as silica nanoparticles, microfluidics, liquid crystals, carbon nanotubes which could be integrated with sensor technology in order to detect microorganisms. In many of these publications antibodies were used as recognition elements by means of specific interactions between the target cell and the binding site of the antibody for the purpose of cell recognition and detection. Even though natural antibodies have high selectivity and sensitivity, they have limited stability and tend to denature in conditions outside the physiological range. Among different approaches, biomimetic materials having superior properties have been used in creating artificial systems. Molecular imprinting is a well suited technique serving the purpose to develop highly selective sensing devices. Molecularly imprinted polymers defined as artificial recognition elements are of growing interest for applications in several sectors of life science involving the investigations on detecting molecules of specific interest. These polymers have attractive properties such as high bio-recognition capability, mechanical and chemical stability, easy preparation and low cost which make them superior over natural recognition reagents. This review summarizes the recent advances in the detection and quantification of microorganisms by emphasizing the molecular imprinting technology and its applications in the development of sensor strategies.

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Untitled

Cited by 23 publications

References 12 publications

Imprinting Technology in Electrochemical Biomimetic Sensors

Imprinting Technology in Electrochemical Biomimetic Sensors

The Effect of Protein Structural Conformation on Nanoparticle Molecular Imprinting of Ribonuclease A Using Miniemulsion Polymerization

Imprinting of Microorganisms for Biosensor Applications

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