W1716 Stimulation By Bradykinin of HCO3- Secretion in Rat Gastroduodenal Mucosa

Aihara, Eitaro; Ohashi, Yumi; Kato, Shinichi; Takeuchi, Koji

doi:10.1016/s0016-5085(08)63271-0

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“…HCO 3 -secretion was measured in a chambered stomach or a duodenal loop, according to the previously published methods (Takeuchi et al, 1991;Aihara et al, 2006). In brief, the stomach was exposed, mounted on a chamber (exposed area: 3.1 cm 2 for rats) and perfused with saline that was gassed with 100 % O 2 and kept in a reservoir.…”

Section: Determination Of Hco 3 -Secretionmentioning

confidence: 99%

Stimulatory effect of Coca-ColaTM on gastroduodenal HCO3− secretion in rats

et al. 2007

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We examined the effect of various carbonated beverages, especially Coca-Cola, on the HCO3- secretion in the rat stomach and duodenum. Under urethane anaesthesia, a chambered stomach or a proximal duodenal loop was perfused with saline, and HCO3- secretion was measured at pH 7.0 using a pH-stat method and by adding 2 mM HCl. The amount of CO2 contained in these beverages was about 4-7 g/mL. Coca-Cola topically applied to the mucosa for 10 min significantly increased the HCO3- secretion in both the stomach and the duodenum. The HCO3- response in the duodenum was totally abolished by indomethacin and also partially inhibited by acetazolamide, an inhibitor of carbonic anhydrase. Likewise, the response in the stomach was also markedly inhibited by either acetazolamide or indomethacin. The mucosal application of Coca-Cola increased the PGE2 contents in both the stomach and the duodenum. Other carbonated beverages, such as sparkling water, Fanta Grape or cider, also increased the HCO3- secretion in these tissues. These results suggest that Coca-Cola induces HCO3- secretion in both the stomach and the duodenum, and these responses may be attributable to both the intracellular supply of HCO3- generated via carbonic anhydrase, and endogenous PGs, probably related to the acidic pH of the solution.

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Section: Determination Of Hco 3 -Secretionmentioning

confidence: 99%