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Abstract

The efficacy of clove oil as an anaesthetic was evaluated in Juvenile of Russian sturgeon (Acipenser gueldenstaedtii) under two experiments. Dose response examined using four anaesthetic concentrations varying from 0.22 to 0.90 g/L of clove oil and effect of exposure duration on the recovery time analysed for 5 and 10 min. Mean body length (cm), 24.9±1.37 and weight (g) 63.2±10.89, n=120, juvenile Russian sturgeon placed individually to the anaesthetic bath prepared with clove oil, and induction (I1: Sedation and I2: Loss of equilibrium) and recovery times (R1: Regain of equilibrium and R2: Sedation) recorded in seconds. Allowing fish remain in the anaesthesia after fish reaching anaesthesia stage, time to recovery from anaesthesia recorded. Fish exposed to clove oil failured to respond to external stimuli and reached to anaesthesia, sympocal stage in <3 min and recovered in <5 min at all doses except 0.22 g/L dose of clove oil. Exposure five and ten minute to anaesthesia at the 0.22, 0.45 and 0.90 g/L concentrations, fish recovered from anaesthesia in <10 min, except 0.90 g/L. The higher dose of clove oil resulted in significantly shorter induction time and prolonged recovery time. While correlation between induction time and dose determined as negative, r= -0.594 and a positive correlation has been found between dose and recovery time, r=0.422 (P<0.05). The study demonstrated that clove oil can be used as an effective anaesthetic measuring length and weight of juvenile Russian sturgeon and dose and exposure duration effect on time to recovery from anaesthesia of clove oil.Keywords: Anaesthesia, clove oil, dose response, exposure duration, recovery time. Karaca Mersinlerinin Karanfil Yağı ile Bayıltılmasında Anesteziye Maruz Kalma Süresinin Ayılmaya Etkisi ÖzetKaraca mersinlerinde (Acipenser gueldenstaedtii) karanfil yağının anestetik etkisi iki çalışma ile ölçülmüştür. Doz tepkisi 0,22'den 0,90 g/L'ye kadar değişen dört farklı konsantrasyonda ölçülmüş ve anesteziye 5 ve 10 dakika maruz kalmanın anesteziden kurtulmaya etkisi incelenmiştir. Ortalama boyları 24,9±1,37 cm ve ağırlıkları 63,2±10,89 g olan karaca mersinleri karanfil yağı ile hazırlanan bayıltıcı solüsyona tek tek yerleştirilmiştir. Her balığın bayılma ( I1: hafif bayılma ve I2: tam bayılma) ve ayılma (R1: hafif ayılma ve R2: tam ayılma) süreleri saniye düzeyinde kayıt edilmiştir. Balıklar tam bayıldıktan sonra anestetik solüsyon içinde bekletilmiş ve ayılma süreleri kayıt edilmiştir. Karanfil yağına maruz kalan balıklar, 0,22 g/L hariç diğer dozlarda 3 dakikadan az sürede bayılarak dış uyarıya tepki vermemiş ve beş dakikadan az sürede ayılmışlardır. Anesteziye 5 ve 10 dakika maruz kalan balıklar 0,22, 0,45 ve 0,90 g/L dozlarda, 0,90 g/L doz hariç, 10 dakika içinde ayılmışlardır. Karanfil yağı dozu arttıkça bayılma süresi kısalmış ve ayılma süresi uzamıştır. Bayılma süresi ile doz arasında negatif r= -0,594 ve doz ile ayılma süresi arasında pozitif r=0,422 (P<0,05) ilişki bulunmuştur. Bu çalışma karanfil yağının karaca mersinlerinin boylarını...

Culture suitability of stinging catfish Heteropneustes fossilis in homestead tank: Selection of suitable stocking size

“…However, fish in treatment T 3 showed more homogeneity among them might be due to similar growth pattern. Collaborated with the FW and WG, significantly higher SGR was recorded in treatment T 1 followed by T 2 and T 3 which contradicts with the findings of Akbulut et al (2002) who reported that specific growth rate decreased with the increasing body size due to the faster growth rate of larger fish. SGR recorded in the present experiment was slightly lower than the findings of Kohinoor et al (2012) who also used the fish with similar initial weight (3.24 to 3.26 g) of our study.…”

Anesthetic protocol for microinjection-related handling of Siberian sturgeon (Acipenser baerii; Acipenseriformes) prolarvae

“…Due to its natural origin, low price, and ready availability, clove oil (or its main constituent, eugenol) is widely used as an anesthetic for fish species [1, 33, 34]. In Russian sturgeon juveniles, test concentrations of 450 to 900 mg/L were reported to meet the efficacy criteria specified for handling within 3 min, with recovery in 5 min and no mortality [35, 36]. Meanwhile, relatively higher doses of clove oil have been used to induce anesthesia of juveniles and subadults of white sturgeon ( Acipenser transmontanus ), with mean induction and recovery times for 1000 mg/L determined as 1.5 min and 16.3 min, respectively [37].…”

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“…Imanpoor et al (2010) recommended a dose of 400 mg/L of clove oil to best anaesthetise Persian sturgeon (Acipenser persicus). Akbulut et al (2011b) considered an effective concentration to be 450-670 mg l -1 of clove oil for juvenile Russian sturgeon (Acipenser gueldenstaedtii). Feng et al (2011) stated that an effective dose is 120 mg/L of clove oil for juvenile Siberian sturgeon.…”

“…There are plenty of studies available on the anaesthetic dosage of clove oil (Hikasa, et al, 1986;Woody et al, 2002;Woolsey et al, 2004;Akbulut et al, 2011aAkbulut et al, , 2011bFeng et al, 2011) and benzocaine (Oswald, 1978;Mattson and Riple, 1989;Gomes et al, 2001) for pelagic fish species which are regarded as seeing-feeding fish, but comparative studies concerning the optimal dose and the effects of anaesthesia on feed intake for sturgeon species which forage on the bottom by touch with barbells (smelling-feeding fish species) are limited. Soto and Burhanuddin (1995) did, however, observe most rabbitfish (Siganus lineatus) feeding a few hours after anaesthesia with clove oil, Prince and Powell (2000) reported that adult rainbow trout (Oncorhynchus mykiss) fed actively 1 week after anaesthesia with clove oil, Pirhonen and Schreck (2003) stated that steelhead trout (Oncorhynchus mykiss) ate relatively well 4 h after anaesthesia with MS-222, clove oil and CO 2 and Sorum and Damsgard (2004) considered that benzocaine anaesthesia had only a minor and insignificant effect on feed intake in Atlantic salmon (Salmo salar).…”