VvNAC17, a novel stress-responsive grapevine (Vitis vinifera L.) NAC transcription factor, increases sensitivity to abscisic acid and enhances salinity, freezing, and drought tolerance in transgenic Arabidopsis

Ju, Young‐Tae; Yue, Xiaofeng; Zhuo, Min; Wang, Xianhang; Fang, Yulin; Zhang, Junxiang

doi:10.1016/j.plaphy.2019.11.002

Cited by 152 publications

(78 citation statements)

References 43 publications

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“…S4 , Table S2 ). Whereas, the NAC domain containing protein 17 ( VvNAC17 ), a transcription factor involved in the response to several stressors that improve the efficiency of the enzymatic antioxidant defence system 35 , was upregulated at Tf in virus-infected plants (Supplementary Fig. S4 ).…”

Section: Resultsmentioning

confidence: 99%

Biological and molecular interplay between two viruses and powdery and downy mildews in two grapevine cultivars

et al. 2020

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Grapevine may be affected simultaneously by several pathogens whose complex interplay is largely unknown. We studied the effects of infection by two grapevine viruses on powdery mildew and downy mildew development and the molecular modifications induced in grapevines by their multiple interactions. Grapevine fanleaf virus (GFLV) and grapevine rupestris stem pitting-associated virus (GRSPaV) were transmitted by in vitro-grafting to Vitis vinifera cv Nebbiolo and Chardonnay virus-free plantlets regenerated by somatic embryogenesis. Grapevines were then artificially inoculated in the greenhouse with either Plasmopara viticola or Erysiphe necator spores. GFLV-infected plants showed a reduction in severity of the diseases caused by powdery and downy mildews in comparison to virus-free plants. GFLV induced the overexpression of stilbene synthase genes, pathogenesis-related proteins, and influenced the genes involved in carbohydrate metabolism in grapevine. These transcriptional changes suggest improved innate plant immunity, which makes the GFLV-infected grapevines less susceptible to other biotic attacks. This, however, cannot be extrapolated to GRSPaV as it was unable to promote protection against the fungal/oomycete pathogens. In these multiple interactions, the grapevine genotype seemed to have a crucial role: in ‘Nebbiolo’, the virus-induced molecular changes were different from those observed in ‘Chardonnay’, suggesting that different metabolic pathways may be involved in protection against fungal/oomycete pathogens. These results indicate that complex interactions do exist between grapevine and its different pathogens and represent the first study on a topic that still is largely unexplored.

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Section: Resultsmentioning

confidence: 99%

Biological and molecular interplay between two viruses and powdery and downy mildews in two grapevine cultivars

et al. 2020

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“…Furthermore, we found that overexpression of VvABF2/bZIP39 in A. thaliana enhanced drought tolerance 11 and could transiently transactivate the expression of VvNAC17 48,49 . Recently, it was reported that heterologous overexpression of VvNAC17 enhanced drought tolerance in transgenic A. thaliana 50 . Taken together, these results suggest that VlbZIP30 binds directly to the promoter of VvNAC17 and regulates its expression to increase drought resistance; in addition, VlbZIP30 also promotes lignin biosynthesis by directly activating VvPRX N1 expression to improve the drought resistance of grapevine.…”

Section: Discussionmentioning

confidence: 99%

Grapevine VlbZIP30 improves drought resistance by directly activating VvNAC17 and promoting lignin biosynthesis through the regulation of three peroxidase genes

Wang

Yin

et al. 2020

Hortic Res

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Drought stress severely affects grapevine quality and yield, and recent reports have revealed that lignin plays an important role in protection from drought stress. Since little is known about lignin-mediated drought resistance in grapevine, we investigated its significance. Herein, we show that VlbZIP30 mediates drought resistance by activating the expression of lignin biosynthetic genes and increasing lignin deposition. Transgenic grapevine plants overexpressing VlbZIP30 exhibited lignin deposition (mainly G and S monomers) in the stem secondary xylem under control conditions, which resulted from the upregulated expression of VvPRX4 and VvPRX72. Overexpression of VlbZIP30 improves drought tolerance, characterized by a reduction in the water loss rate, maintenance of an effective photosynthesis rate, and increased lignin content (mainly G monomer) in leaves under drought conditions. Electrophoretic mobility shift assay, luciferase reporter assays, and chromatin immunoprecipitation-qPCR assays indicated that VlbZIP30 directly binds to the G-box cis-element in the promoters of lignin biosynthetic (VvPRX N1) and drought-responsive (VvNAC17) genes to regulate their expression. In summary, we report a novel VlbZIP30-mediated mechanism linking lignification and drought tolerance in grapevine. The results of this study may be of value for the development of molecular breeding strategies to produce drought-resistant fruit crops.

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“…Among them, several examples are miR156-mediated cleavage of SPLs, miR164-mediated cleavage of NACs, miR319-mediated cleavage of TCPs and miR396-mediated cleavage of GRF genes. Previous studies have shown that NAC family members (NAC1, NAC4, NAC13 and NAC17) play important regulatory roles in plant responses to salt stress via activating the expression of stress-responsive genes [ 38 – 41 ]. In our present work, we found that the level of NAC 21 and NAC79 mRNA increased compared with the decrease in miR164d and miR164e in sesame under salt stress, suggesting their important roles in sesame (Fig.…”

Section: Discussionmentioning

confidence: 99%

Integrated small RNA and Degradome sequencing provide insights into salt tolerance in sesame (Sesamum indicum L.)

Zhang

Gong

et al. 2020

BMC Genomics

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Background: MicroRNAs (miRNAs) exhibit important regulatory roles in the response to abiotic stresses by posttranscriptionally regulating the target gene expression in plants. However, their functions in sesame response to salt stress are poorly known. To dissect the complex mechanisms underlying salt stress response in sesame, miRNAs and their targets were identified from two contrasting sesame genotypes by a combined analysis of small RNAs and degradome sequencing. Results: A total of 351 previously known and 91 novel miRNAs were identified from 18 sesame libraries. Comparison of miRNA expressions between salt-treated and control groups revealed that 116 miRNAs were involved in salt stress response. Using degradome sequencing, potential target genes for some miRNAs were also identified. The combined analysis of all the differentially expressed miRNAs and their targets identified miRNA-mRNA regulatory networks and 21 miRNA-mRNA interaction pairs that exhibited contrasting expressions in sesame under salt stress. Conclusions: This comprehensive integrated analysis may provide new insights into the genetic regulation mechanism of miRNAs underlying the adaptation of sesame to salt stress.

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VvNAC17, a novel stress-responsive grapevine (Vitis vinifera L.) NAC transcription factor, increases sensitivity to abscisic acid and enhances salinity, freezing, and drought tolerance in transgenic Arabidopsis

Cited by 152 publications

References 43 publications

Biological and molecular interplay between two viruses and powdery and downy mildews in two grapevine cultivars

Biological and molecular interplay between two viruses and powdery and downy mildews in two grapevine cultivars

Grapevine VlbZIP30 improves drought resistance by directly activating VvNAC17 and promoting lignin biosynthesis through the regulation of three peroxidase genes

Integrated small RNA and Degradome sequencing provide insights into salt tolerance in sesame (Sesamum indicum L.)

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