‘VT Sweet’: A vegetable soybean cultivar for commercial edamame production in the mid‐Atlantic USA

Zhang, Bo; Lord, Nilanka; Kuhar, Thomas P.; Duncan, Susan E.; Huang, Haibo; Ross, Jeremy; Rideout, Steven L.; Arancibia, Ramón A.; Reiter, Mark S.; Song, Li; Chen, Pengyin; Mozzoni, Leandro; Gillen, Anne M.; Yin, Yanlong; Neill, Clinton L.; Carneiro, Renata; Yu, Dajun; Sutton, Kemper L; Li, Xiaoying; Wang, Zhibo; Buss, G. R.

doi:10.1002/plr2.20140

Cited by 9 publications

(7 citation statements)

References 8 publications

(12 reference statements)

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“…The three "sweet" accessions characterized in present study had the comparable sucrose content but significantly high Ala content. By contrast, VT Sweet, a recent released edamame cultivar (Zhang et al, 2021), had 69.22 ± 21.65 mg sucrose g -1 fresh bean and 0.59 ± 0.07 mg Ala g -1 fresh bean in the Blacksburg extension trial, 2019. PI532469 and PI243551 both originated from Japan and PI407748 originated from China.…”

Section: Potential Edamame Breeding Pismentioning

confidence: 94%

Genome-wide association analysis of sucrose and alanine contents in edamame beans

Wang

Yu²,

Morota³

et al. 2023

Front. Plant Sci.

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The sucrose and Alanine (Ala) content in edamame beans significantly impacts the sweetness flavor of edamame-derived products as an important attribute to consumers’ acceptance. Unlike grain-type soybeans, edamame beans are harvested as fresh beans at the R6 to R7 growth stages when beans are filled 80-90% of the pod capacity. The genetic basis of sucrose and Ala contents in fresh edamame beans may differ from those in dry seeds. To date, there is no report on the genetic basis of sucrose and Ala contents in the edamame beans. In this study, a genome-wide association study was conducted to identify single nucleotide polymorphisms (SNPs) related to sucrose and Ala levels in edamame beans using an association mapping panel of 189 edamame accessions genotyped with a SoySNP50K BeadChip. A total of 43 and 25 SNPs was associated with sucrose content and Ala content in the edamame beans, respectively. Four genes (Glyma.10g270800, Glyma.08g137500, Glyma.10g268500, and Glyma.18g193600) with known effects on the process of sucrose biosynthesis and 37 novel sucrose-related genes were characterized. Three genes (Gm17g070500, Glyma.14g201100 and Glyma.18g269600) with likely relevant effects in regulating Ala content and 22 novel Ala-related genes were identified. In addition, by summarizing the phenotypic data of edamame beans from three locations in two years, three PI accessions (PI 532469, PI 243551, and PI 407748) were selected as the high sucrose and high Ala parental lines for the perspective breeding of sweet edamame varieties. Thus, the beneficial alleles, candidate genes, and selected PI accessions identified in this study will be fundamental to develop edamame varieties with improved consumers’ acceptance, and eventually promote edamame production as a specialty crop in the United States.

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Section: Potential Edamame Breeding Pismentioning

confidence: 94%

Genome-wide association analysis of sucrose and alanine contents in edamame beans

Wang

Yu²,

Morota³

et al. 2023

Front. Plant Sci.

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“…To achieve this goal, we applied Oxford Nanopore Long-Read Sequencing Technology (ONT) to sequence 28 representative genotypes of both grain-type soybean and vegetable soybean (edamame) as well as the reference variety, William 82 (W82, Table S1). This germplasm compilation includes grain-type soybeans such as Hutcheson and V12-4590 used in livestock feeding, food-grade soybeans like zizuka (natto), V10-3653 (soy milk and tofu producing variety), MFS-561 (sprout variety), and vegetable soybeans (edamame) including VT-Sweet and V16-0565 9-12 . These genotypes feature diverse traits such as high methionine content, high yield, varying seed protein levels, distinct plant heights (both high and low), and high oleic acid content, and different seed weights (Figure 1 and Table S1).Our study doubled the number of soybean genotypes that were re-sequenced by a long-reading sequencing approach 4 .…”

Section: Mainmentioning

confidence: 99%

Long-read sequencing reveals novel structural variation markers for key agronomic and quality traits of soybean

Wang,

Belay,

Paterson

et al. 2024

Preprint

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In plant genomic research, long read sequencing has been widely used to detect structure variations that are not captured by short read sequencing. In this letter, we described an analysis of whole genome re-sequencing of 29 soybean varieties using nanopore long-read sequencing. The compiled germplasm reflects diverse applications, including livestock feeding, soy milk and tofu production, as well as consumption of natto, sprouts, and vegetable soybeans (edamame). We have identified 365,497 structural variations in these newly re-sequenced genomes and found that the newly identified structural variations are associated with important agronomic traits. These traits include seed weight, flowering time, plant height, oleic acid content, methionine content, and trypsin inhibitor content, all of which significantly impact soybean production and quality. Experimental validation supports the roles of predicted candidate genes and structural variant in these biological processes. Our research provides a new source for rapid marker discovery in crop genomes using structural variation and whole genome sequencing.

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“…Vegetable soybeans are harvested at the R6 stage, when the pods are green and the seeds are full [2] . Research has reported that the pods size, including large pods and grains, is an important visual quality unique to vegetable soybeans [3,4]. Therefore, the size of vegetable soybean pods has been considered to be one of the most important traits in accelerating the breeding process of vegetable soybean.…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Association Analysis for Yield-Related Traits and Candidate Genes in Vegetable Soybean

Gao,

Wu,

et al. 2024

Preprint

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Owing to the rising demand for vegetable soybean products, there is an increasing need for high-yield soybean varieties to raise the output. However, complex correlation patterns among quantitative traits with genetic interactions bring a challenge for vegetable soybean breeding. Here, a genome-wide association study (GWAS) was applied for six yield-related traits with 188 vegetable soybean accessions. A total of 81 SNPs were identified for pod length, plant height, pods number, pod thickness, pod width, and seed weight per plant by Blink model. Among these significant SNPs, 79 were novel SNPs, while 2 overlapped with previously reported SNPs. A total of 220 genes were found in 100 kb upstream and downstream regions of significant SNPs, of which 17 genes were functional proteins. Among these function proteins, four candidate genes, Glyma.13G109100, Glyma.03G183200, Glyma.09G102200, and Glyma.09G102300 were analyzed as significant haplotype variations, which encode MYB-related transcription factor, auxin-responsive family protein, F-box protein and CYP450, respectively. In addition, the relative expression with four candidate genes in the V030 and V071 (Among them, the plant height, pod number and fresh pod weight of V030 were lower than V071 strains) vegetable soybean were significantly different and these genes could be involved in plant growth and development via various pathways. Therefore, we proposed four genes from vegetable soybean germplasm that could be the key candidate genes for pod yield and plant height and these studies will accelerate the process of high-yield vegetable soybean breeding.

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‘VT Sweet’: A vegetable soybean cultivar for commercial edamame production in the mid‐Atlantic USA

Cited by 9 publications

References 8 publications

Genome-wide association analysis of sucrose and alanine contents in edamame beans

Genome-wide association analysis of sucrose and alanine contents in edamame beans

Long-read sequencing reveals novel structural variation markers for key agronomic and quality traits of soybean

Genome-Wide Association Analysis for Yield-Related Traits and Candidate Genes in Vegetable Soybean

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