VSL#3 Probiotic-Mixture Induces Remission in Patients with Active Ulcerative Colitis

Bibiloni, Rodrigo; Fedorak, Richard N.; Tannock, Gerald W.; Madsen, Karen; Gionchetti, Paolo; Campieri, Massimo; Simone, Claudio De; Sartor, R. Balfour

doi:10.1111/j.1572-0241.2005.41794.x

Cited by 653 publications

(395 citation statements)

References 41 publications

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“…Selected probiotics have been shown to be effective for some gastrointestinal disorders in humans and domestic animals yet prevention of neonatal diarrhea has not been reported 27, 28. Further, successful probiotic studies have often involved inflammatory, not infectious, disorders,29, 30 raising questions of the potential efficacy of probiotics in foals given the pathophysiology of foal diarrhea. Recent advances in the knowledge of the equine intestinal microbiota could be used to design equine‐specific probiotics and further investigation of this area is required.…”

Section: Discussionmentioning

confidence: 99%

Effect of a Probiotic on Prevention of Diarrhea and Clostridium difficile and Clostridium perfringens Shedding in Foals

Schoster

Staempfli

Abrahams

et al. 2015

Veterinary Internal Medicne

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BackgroundUp to 60% of foals develop diarrhea within 6 months after birth. Preventive measures are limited but potentially probiotics could be used.ObjectiveTo evaluate the effect of a newly designed probiotic on the incidence of foal diarrhea in a randomized field trial.AnimalsSeventy‐two healthy neonatal foals.MethodsRandomized, placebo‐controlled field trial. Foals were administered a placebo or probiotic for 3 weeks and monitored for an additional week. A total of 3 fecal samples were taken from each foal at biweekly intervals. Statistical modeling was applied for comparison of incidence and duration of diarrhea and fecal shedding of Clostridium perfringens and Clostridium difficile between treatment and age groups.ResultsThe overall incidence of diarrhea was 41 of 72 (59%) and did not differ (P = 0.37) between treatment groups. Foals treated with probiotics were more likely to develop diarrhea requiring veterinary intervention (P = 0.007). Age had a significant effect on incidence of diarrhea (P < 0.001); foals 8–15 days old having the highest probability of developing diarrhea. Duration of diarrhea and soft feces were not significantly different between groups. The prevalence of C. perfringens shedding was 55% with no difference between treatment groups (P = 0.23). The prevalence of C. difficile shedding was 11%.Conclusion and Clinical ImportanceThere was no benefit of administering a 3‐week course of probiotics, but potential adverse effects were noted. Whether the probiotics lacked a clinical effect, or the choice of strains or dose was inadequate, is unknown. Clostridial shedding was not influenced by probiotics despite in vitro activity of probiotics.

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Section: Discussionmentioning

confidence: 99%

Effect of a Probiotic on Prevention of Diarrhea and Clostridium difficile and Clostridium perfringens Shedding in Foals

Schoster

Staempfli

Abrahams

et al. 2015

Veterinary Internal Medicne

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“…There is evidence that probiotic treatment is effective against UC [3][4][5][6] . We have previously shown that treatment with bifidobacteriafermented milk (BFM) containing live bifidobacteria is more effective than the usual treatment in inducing [7] and maintaining remission [8] of ulcerative colitis.…”

Section: Introductionmentioning

confidence: 99%

Anti-inflammatory activity of probiotic Bifidobacterium: Enhancement of IL-10 production in peripheral blood mononuclear cells from ulcerative colitis patients and inhibition of IL-8 secretion in HT-29 cells

Imaoka¹,

Shima²,

Kato³

et al. 2008

WJG

152

100

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AIM:To determine the anti-inflammatory activity of probiotic Bifidobacteria in Bifidobacteria-fermented milk (BFM) which is effective against active ulcerative colitis (UC) and exacerbations of UC, and to explore the immunoregulatory mechanisms. METHODS:Peripheral blood mononuclear cells (PBMNC) from UC patients or HT-29 cells were co-cultured with heat-killed probiotic bacteria or culture supernatant of Bifidobacterium breve strain Yakult (BbrY) or Bifidobacterium bifidum strain Yakult (BbiY) to estimate the amount of IL-10 or IL-8 secreted.RESULTS: Both strains of probiotic Bifidobacteria contained in the BFM induced IL-10 production in PBMNC from UC patients, though BbrY was more effective than BbiY. Conditioned medium (CM) and DNA of both strains inhibited IL-8 secretion in HT-29 cells stimulated with TNF-α, whereas no such effect was observed with heatkilled bacteria. The inhibitory effect of CM derived from BbiY was greater than that of CM derived from BbrY. DNAs of the two strains had a comparable inhibitory activity against the secretion of IL-8. CM of BbiY induced a repression of IL-8 gene expression with a higher expression of IκB-ζ mRNA 4 h after culture of HT-29 cells compared to that in the absence of CM. CONCLUSION:Probiotic Bifidobacterium strains in BFM enhance IL-10 production in PBMNC and inhibit IL-8 secretion in intestinal epithelial cells, suggesting that BFM has anti-inflammatory effects against ulcerative colitis.

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“…VSL#3 is a probiotic mixture of eight different bacterial strains that has demonstrated beneficial effects in several intestinal conditions. Controlled studies have shown VSL#3 to be efficacious for patients with ulcerative colitis (UC) and pouchitis (8,9), as well as in animal models of colitis (10,11). However, the precise mechanisms of action are not fully understood.…”

mentioning

confidence: 99%

Probiotics promote gut health through stimulation of epithelial innate immunity

Pagnini

Saeed

Bamias

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

297

223

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Probiotic formulations are widely available and have a variety of proposed beneficial effects, including promotion of gut health. The mechanisms of action of probiotic bacteria in the intestine are still unclear but are generally attributed to an antiinflammatory effect. Here, we demonstrate that the multiple probiotic formulation VSL#3 prevents the onset of intestinal inflammation by local stimulation of epithelial innate immune responses (i.e., increased production of epithelial-derived TNF-α and restoration of epithelial barrier function in vivo). We also demonstrate that probiotic bacteria stimulate epithelial production of TNF-α and activate NF-κB in vitro. Our results support the hypothesis that probiotics promote gut health through stimulation, rather than suppression, of the innate immune system. Furthermore, our findings provide the perspective that defects in innate immunity may play a critical role in the pathogenesis and progression of intestinal disorders, such as inflammatory bowel disease.

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VSL#3 Probiotic-Mixture Induces Remission in Patients with Active Ulcerative Colitis

Cited by 653 publications

References 41 publications

Effect of a Probiotic on Prevention of Diarrhea and Clostridium difficile and Clostridium perfringens Shedding in Foals

Effect of a Probiotic on Prevention of Diarrhea and Clostridium difficile and Clostridium perfringens Shedding in Foals

Anti-inflammatory activity of probiotic Bifidobacterium: Enhancement of IL-10 production in peripheral blood mononuclear cells from ulcerative colitis patients and inhibition of IL-8 secretion in HT-29 cells

Probiotics promote gut health through stimulation of epithelial innate immunity

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