Voxx: a PC-based, near real-time volume rendering  system for biological microscopy

Clendenon, Jeffrey L.; Phillips, Carrie L.; Sandoval, Ruben M.; Fang, Shiaofen; Dunn, Kenneth W.

doi:10.1152/ajpcell.2002.282.1.c213

Cited by 123 publications

(98 citation statements)

References 4 publications

(3 reference statements)

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“…Images were obtained using a Zeiss Pascal confocal microscope. Voxx rendering software (Clendenon et al, 2002) was used for 3D reconstruction. LacZ expression in whole mounts of TS muscles from heterozygous tenascin-C-null mutant mice was detected by X-gal staining solution, with a solution containing 2 mM MgCl 2 , 5 mM K 3 Fe(CN) 6 , 5 mM 0.02% NP-40 and 1 mg/ml X-gal (5-bromo-4-chloro-3-indolyl-D-galactoside; Sigma).…”

Section: Immunocytochemistry and Histochemistrymentioning

confidence: 99%

Identity, developmental restriction and reactivity of extralaminar cells capping mammalian neuromuscular junctions

Court

Gillingwater

Melrose

et al. 2008

Journal of Cell Science

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Neuromuscular junctions (NMJs) are normally thought to comprise three major cell types: skeletal muscle fibres, motor neuron terminals and perisynaptic terminal Schwann cells. Here we studied a fourth population of junctional cells in mice and rats, revealed using a novel cytoskeletal antibody (2166). These cells lie outside the synaptic basal lamina but form caps over NMJs during postnatal development. NMJ-capping cells also bound rPH, HM-24, CD34 antibodies and cholera toxin B subunit. Bromodeoxyuridine incorporation indicated activation, proliferation and spread of NMJ-capping cells following denervation in adults, in advance of terminal Schwann cell sprouting. The NMJ-capping cell reaction coincided with expression of tenascin-C but was independent of this molecule because capping cells also dispersed after denervation in tenascin-C-null mutant mice. NMJ-capping cells also dispersed after local paralysis with botulinum toxin and in atrophic muscles of transgenic R6/2 mice. We conclude that NMJ-capping cells (proposed name `kranocytes') represent a neglected, canonical cellular constituent of neuromuscular junctions where they could play a permissive role in synaptic regeneration.

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Section: Immunocytochemistry and Histochemistrymentioning

confidence: 99%

Identity, developmental restriction and reactivity of extralaminar cells capping mammalian neuromuscular junctions

Court

Gillingwater

Melrose

et al. 2008

Journal of Cell Science

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“…Montages were assembled and annotated using Photoshop (Adobe, Mountain View, CA). Volume renderings were conducted using Voxx, a PC-based image analysis program developed at the Indiana Center for Biological Microscopy (Clendenon et al, 2002) and available at no charge (http://nephrology.iupui.edu/imaging/voxx).…”

Section: Image Processing For Presentationmentioning

confidence: 99%

Rab10 Regulates Membrane Transport through Early Endosomes of Polarized Madin-Darby Canine Kidney Cells

Babbey

Ahktar

Wang

et al. 2006

MBoC

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“…A series of optical section comprising the complete volume of the tectal arborization was collected, and these images were rendered to create a three-dimensional volume that could be viewed at any orientation using voxel rendering software, Voxx (Clendenon et al, 2002). Inspection of these volumes showed that control retinotectal arborizations were extensive and finely branched ( Fig.…”

Section: Defective Retinotectal Projection In Cdh4 Knockdown Embryosmentioning

confidence: 99%

Zebrafish R‐cadherin (Cdh4) controls visual system development and differentiation

Babb

Kotradi

Shah

et al. 2005

Developmental Dynamics

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In zebrafish, R-cadherin (cadherin-4 or Cdh4) is expressed in the retina and in retinorecipient brain regions, suggesting that Cdh4 functions during visual system development. Cdh4 function was examined during retinogenesis and retinal axon outgrowth using antisense morpholino oligonucleotides and mutant Cdh4 construct expression. In knockdowns, Cdh4 was reduced or absent, eyes were small, and retinae lacked discrete laminae. Increased cell death produced the small eye phenotype. Zn5-, Pax6-, and zpr-1-positive cells were reduced or absent in knockdown retinas but, when present, were in the correct laminae. Cdh4 knockdowns had sparse or absent retinal ganglion cell axons. When present, axons projected contralaterally but lacked fine branching and failed to reach the tectum or arborize the entire tectum. Mutant Cdh4 construct expression during retinal ganglion cell differentiation reduced or ablated neurite formation. Cdh4 is necessary for neural retina survival and differentiation, and required for normal retinotectal projection formation and tectal arborization. Developmental Dynamics 233:930 -945, 2005.

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Voxx: a PC-based, near real-time volume rendering system for biological microscopy

Cited by 123 publications

References 4 publications

Identity, developmental restriction and reactivity of extralaminar cells capping mammalian neuromuscular junctions

Identity, developmental restriction and reactivity of extralaminar cells capping mammalian neuromuscular junctions

Rab10 Regulates Membrane Transport through Early Endosomes of Polarized Madin-Darby Canine Kidney Cells

Zebrafish R‐cadherin (Cdh4) controls visual system development and differentiation

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