Vortex-free high-Reynolds deterministic lateral displacement (DLD) via airfoil pillars

Dincau, Brian; Aghilinejad, Arian; Chen, Xiaolin; Moon, Se Youn; Kim, Jong Hoon

doi:10.1007/s10404-018-2160-3

Cited by 30 publications

(30 citation statements)

References 19 publications

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“…In addition, it has been recently shown by Zeming et al that utilizing asymmetrical gaps for DLD device enhanced the separation of red blood cells without restricting the throughput significantly [20]. However, DLD faces major design challenges such as inability to sort cells with similar size and unpredictability of the device performance at high throughputs due to flow-induced cell deformation [17,21,22]. There have been prior attempts to couple DLD and DEP although none of them investigated the applicability of such concept for continuous CTC separation.…”

Section: Introductionmentioning

confidence: 99%

On the design of deterministic dielectrophoresis for continuous separation of circulating tumor cells from peripheral blood cells

et al. 2019

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Detection and analysis of circulating tumor cells (CTCs) have emerged as a promising way to diagnose cancer, study its cellular mechanism, and test or develop potential treatments. However, the rarity of CTCs among peripheral blood cells is a big challenge toward CTC detection. In addition, in cases where there is similar size range between certain types of CTCs (e.g. breast cancer cells) and white blood cells (WBCs), high‐resolution techniques are needed. In the present work, we propose a deterministic dielectrophoresis (DEP) method that combines the concept of deterministic lateral displacement (DLD) and insulator‐based dielectrophoresis (iDEP) techniques that rely on physical markers such as size and dielectric properties to differentiate different type of cells. The proposed deterministic DEP technology takes advantage of frequency‐controlled AC electric field for continuous separation of CTCs from peripheral blood cells. Utilizing numerical modeling, different aspects of coupled DLD‐DEP design such as the required applied voltages, velocities, and geometrical parameters of DLD arrays of microposts are investigated. Regarding the inevitable difference and uncertainty ranges for the reported crossover frequencies of cells, a comprehensive analysis is conducted on applied electric field frequency as design's determinant factor. Deterministic DEP design provides continuous sorting of CTCs from WBCs even with similar size and has the future potential for high throughput and efficiency.

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Section: Introductionmentioning

confidence: 99%

On the design of deterministic dielectrophoresis for continuous separation of circulating tumor cells from peripheral blood cells

et al. 2019

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“…This may be employed for the development of DLD devices with an adjustable gap between the posts with the separation threshold tuned by the fluid flow rate. Further studies revealed that the main mechanism responsible for switching the displacement mode is associated with the compressing of streamlines under high-Re [327]. This was shown by the separation of vortex formation and streamline compression using the posts of a symmetric airfoil shape that does not produce the vortices even under Re = 100.…”

Section: Deterministic Lateral Displacementmentioning

confidence: 99%

Detection of Rare Objects by Flow Cytometry: Imaging, Cell Sorting, and Deep Learning Approaches

Voronin

Kozlova

Verkhovskii

et al. 2020

IJMS

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Flow cytometry nowadays is among the main working instruments in modern biology paving the way for clinics to provide early, quick, and reliable diagnostics of many blood-related diseases. The major problem for clinical applications is the detection of rare pathogenic objects in patient blood. These objects can be circulating tumor cells, very rare during the early stages of cancer development, various microorganisms and parasites in the blood during acute blood infections. All of these rare diagnostic objects can be detected and identified very rapidly to save a patient’s life. This review outlines the main techniques of visualization of rare objects in the blood flow, methods for extraction of such objects from the blood flow for further investigations and new approaches to identify the objects automatically with the modern deep learning methods.

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“…16(f)]. 176 By adopting triangular micropost arrays [Fig. 16(g)], clogging issues in the DLD device were significantly mitigated as a result of the larger working gap size between triangular microposts.…”

Section: Sorting By Deterministic Lateral Migrationmentioning

confidence: 99%

“…To avoid generation of vortex and compressed streamlines, low “Reflow” and thus low throughput are generally necessary 177 . Nevertheless, a much higher throughput has been achieved in a DLD device with symmetric airfoil pillars in a recent work 176 . Vortices were not observed at Re up to 100, and 20 μ m diameter particles were successfully separated from 10 μ m and 15 μ m particle mixtures with an efficiency of ∼100% 176 …”

Section: Sorting By Deterministic Lateral Migrationmentioning

confidence: 99%

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Label-free microfluidic sorting of microparticles

et al. 2019

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Massive growth of the microfluidics field has triggered numerous advances in focusing, separating, ordering, concentrating, and mixing of microparticles. Microfluidic systems capable of performing these functions are rapidly finding applications in industrial, environmental, and biomedical fields. Passive and label-free methods are one of the major categories of such systems that have received enormous attention owing to device operational simplicity and low costs. With new platforms continuously being proposed, our aim here is to provide an updated overview of the state of the art for passive label-free microparticle separation, with emphasis on performance and operational conditions. In addition to the now common separation approaches using Newtonian flows, such as deterministic lateral displacement, pinched flow fractionation, cross-flow filtration, hydrodynamic filtration, and inertial microfluidics, we also discuss separation approaches using non-Newtonian, viscoelastic flow. We then highlight the newly emerging approach based on shear-induced diffusion, which enables direct processing of complex samples such as untreated whole blood. Finally, we hope that an improved understanding of label-free passive sorting approaches can lead to sophisticated and useful platforms toward automation in industrial, environmental, and biomedical fields.

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Vortex-free high-Reynolds deterministic lateral displacement (DLD) via airfoil pillars

Cited by 30 publications

References 19 publications

On the design of deterministic dielectrophoresis for continuous separation of circulating tumor cells from peripheral blood cells

On the design of deterministic dielectrophoresis for continuous separation of circulating tumor cells from peripheral blood cells

Detection of Rare Objects by Flow Cytometry: Imaging, Cell Sorting, and Deep Learning Approaches

Label-free microfluidic sorting of microparticles

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