Vortex-assisted surfactant-enhanced emulsification microextraction combined with LC–MS/MS for the determination of glucocorticoids in water with the aid of experimental design

Asati, Ankita; Satyanarayana, G.N.V.; Patel, Devendra Kumar

doi:10.1007/s00216-017-0236-7

Cited by 12 publications

(4 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The analysis of variance (ANOVA) was used to assess model adequacy and significant variables were identified by using F-test [29]. The polynomial fit quality was expressed by the coefficient [30], in which R 2 = 1 is the best quality and the lowest value for R 2 is set to be higher than 0.8 [31]. In this study, experimental R 2 and adjusted R 2 were determined as 90.33% and 85.17%, respectively for TCPA, and 95.03% and 90.47%, respectively for MRT.…”

Section: Resultsmentioning

confidence: 99%

Detection of Five Mycotoxins in Different Food Matrices in the Malaysian Market by Using Validated Liquid Chromatography Electrospray Ionization Triple Quadrupole Mass Spectrometry

Alsharif

Choo

Tan

2019

Toxins

View full text Add to dashboard Cite

Mycotoxins are common food contaminants which cause poisoning and severe health risks to humans and animals. The present study applied chemometric approach in liquid chromatography-tandem mass spectrometry (LC-MS/MS) optimization for simultaneous determination of mycotoxins, i.e., aflatoxins B1, B2, G1, and G2, and ochratoxin A. The validated quick, easy, cheap, effective, rugged, and safe (QuEChERS)-LC-MS/MS method was used to study the occurrence of mycotoxins in 120 food matrices. The recovery ranges from 81.94% to 101.67% with relative standard deviation (RSD) lesser than 11%. Through the developed method, aflatoxins were detected in raisin, pistachio, peanut, wheat flour, spice, and chili samples with concentration ranges from 0.45 to 16.93 µg/kg. Trace concentration of ochratoxin A was found in wheat flour and peanut samples which ranged from 1.2 to 3.53 µg/kg. Some of the tested food samples contained mycotoxins of above the European legal maximum limit.

show abstract

Section: Resultsmentioning

confidence: 99%

Detection of Five Mycotoxins in Different Food Matrices in the Malaysian Market by Using Validated Liquid Chromatography Electrospray Ionization Triple Quadrupole Mass Spectrometry

Alsharif

Choo

Tan

2019

Toxins

View full text Add to dashboard Cite

show abstract

“…The advantage of using a DoE approach is the possibility to evaluate multiple parameters simultaneously via statistical experiments versus the traditional approach of changing only one parameter at a time in no particular order and observing changes to the results on a run by run basis. Using a DoE approach is widely applied in bioanalytical science, emergency toxicology, environmental analytics, food analytics, , metabolomics, − pharmaceutical analytics, − and water analytics mostly for optimization of chromatographic separation. However, using a DoE approach has not yet been described to master the data overload resulting from modern data independent acquisition methods used for general unknown screening procedures by optimizing software parameters for the feature detection and hit generation.…”

Section: Resultsmentioning

confidence: 99%

“…A more progressive strategy is using a design of experiment (DoE) approach where multiple parameters can be evaluated simultaneously. DoE approaches are already widely applied in bioanalytical science, emergency toxicology, environmental analytics, food analytics, , metabolomics, − pharmaceutical analytics, − and water analytics mostly for optimization of the chromatographic separation.…”

mentioning

confidence: 99%

Parameter Optimization for Feature and Hit Generation in a General Unknown Screening Method—Proof of Concept Study Using a Design of Experiment Approach for a High Resolution Mass Spectrometry Procedure after Data Independent Acquisition

et al. 2018

View full text Add to dashboard Cite

High resolution mass spectrometry and modern data independent acquisition (DIA) methods enable the creation of general unknown screening (GUS) procedures. However, even when DIA is used, its potential is far from being exploited, because often, the untargeted acquisition is followed by a targeted search. Applying an actual GUS (including untargeted screening) produces an immense amount of data that must be dealt with. An optimization of the parameters regulating the feature detection and hit generation algorithms of the data processing software could significantly reduce the amount of unnecessary data and thereby the workload. Design of experiment (DoE) approaches allow a simultaneous optimization of multiple parameters. In a first step, parameters are evaluated (crucial or noncrucial). Second, crucial parameters are optimized. The aim in this study was to reduce the number of hits, without missing analytes. The obtained parameter settings from the optimization were compared to the standard settings by analyzing a test set of blood samples spiked with 22 relevant analytes as well as 62 authentic forensic cases. The optimization lead to a marked reduction of workload (12.3 to 1.1% and 3.8 to 1.1% hits for the test set and the authentic cases, respectively) while simultaneously increasing the identification rate (68.2 to 86.4% and 68.8 to 88.1%, respectively). This proof of concept study emphasizes the great potential of DoE approaches to master the data overload resulting from modern data independent acquisition methods used for general unknown screening procedures by optimizing software parameters.

show abstract

“…A DLLME foi utilizada no preparo de amostras de urina (Jia et al, 2013) e de água superficial (Asati;Satunarayana;Patel, 2017), para determinação multiclasses, incluindo ERI e de macrolídeos, respectivamente. Em análise de alimentos, a técnica foi empregada para avaliação da ocorrência de ERI e seus produtos de degradação em mel (Zhao et al 2017).…”

Section: Técnica De Microextração Líquido-líquido Dispersivaunclassified

Desenvolvimento de método analítico envolvendo QuEChERS modificado e DLLME para a determinação de resíduos de eritromicina em peixe por LC-MS/MS

Campanharo¹

View full text Add to dashboard Cite

Agradeço muito à Deus pela vida, por minha saúde e pela saúde da minha família e de todos que amo.Agradeço à minha família por acreditarem em mim sempre, por estarem presentes nos momentos bons e nos difíceis e pelo amor incondicional. Muito obrigada pelo apoio! Ao meu companheiro Antônio pela parceria, por todo o amor e paciência.Agradeço ao professor Dr. Jonas Paschoal pela confiança e pela oportunidade de fazer parte do seu grupo de pesquisa. Por todos os ensinamentos, todas as conversas e pela orientação. Sempre serei grata.Aos meus colegas de laboratório Juliana, Carol, Carlos, João, Marina e especialmente ao Jonas, ao Lucas e à Thaís, por me ajudarem nas atividades, por terem trabalhado muito comigo, pela amizade e pelos momentos de descontração.Ao técnico do laboratório, Agnaldo, que esteve comigo na realização dos experimentos, compartilhando seu conhecimento, com muita paciência e me ajudando em todos os desafios.Agradeço aos professores que colaboraram no desenvolvimento deste trabalho, em especial à professora Dra. Fabiana Pilarski, do Centro de Aquicultura da UNESP de Jaboticabal e professora Dra. Priscyla D. Gaspari, do laboratório de Nanobiotecnologia.Agradeço ao professor Dr. Jairo Kenupp Bastos do Laboratório de Farmacognosia, pela gentileza de fornecer as amostras de óleo volátil de Copaíba.Agradeço também aos técnicos de outros laboratórios que me auxiliaram: Henrique Dias, Perpétua, Laila, Patrícia e especialmente à Taís. Obrigada por serem tão gentis comigo.Aos alunos da professora Fabiana, especialmente ao Inácio, pela ajuda com os experimentos com os animais, pelo companheirismo, confiança e seriedade em seu trabalho.Às alunas da professora Dra. Priscyla, especialmente à Bianca e à Letícia, por me receberem tão bem no laboratório e pelo auxílio nos testes de desenvolvimento das nanopartículas.Agradeço muito a todos os professores das disciplinas que cursei neste período e aos professores que tive a oportunidade de conhecer em palestras e workshops. Obrigada por tudo que me ensinaram.Agradeço aos funcionários da Faculdade de Ciências Farmacêuticas de Ribeirão Preto, especialmente aos funcionários da pós-graduação, Henrique e Eleni, que sempre foram gentis comigo e se dispuseram a me auxiliar.Agradeço aos colegas de pós-graduação que conheci nesta jornada, especialmente ao Rodolfo, pelo incentivo, pelas conversas e momentos de descontração.Agradeço à FAPESP pelo apoio ao projeto de pesquisa, pelas avaliações, críticas, sugestões e pelo auxílio financeiro (2012/18334-0 e 2019/06159-8) e pela bolsa de estudos concedida (2018/05536-0).Agradeço também ao CNPq, pelo apoio à pós-graduação e pelo auxílio financeiro (132655/2018-7).O presente trabalho foi realizado com apoio da Coordenação de Aperfeiçoamento de Pessoal de Nível Superior -Brasil (CAPES) -Código de Financiamento 001.Muito obrigada a todos! ABSTRACT CAMPANHARO, S. C. Development of an analytical method using modified QuEChERS and DLLME for the determination of erythromycin residues in fish by LC-MS/MS. 2021. 143 p.

show abstract

Vortex-assisted surfactant-enhanced emulsification microextraction combined with LC–MS/MS for the determination of glucocorticoids in water with the aid of experimental design

Cited by 12 publications

References 55 publications

Detection of Five Mycotoxins in Different Food Matrices in the Malaysian Market by Using Validated Liquid Chromatography Electrospray Ionization Triple Quadrupole Mass Spectrometry

Detection of Five Mycotoxins in Different Food Matrices in the Malaysian Market by Using Validated Liquid Chromatography Electrospray Ionization Triple Quadrupole Mass Spectrometry

Parameter Optimization for Feature and Hit Generation in a General Unknown Screening Method—Proof of Concept Study Using a Design of Experiment Approach for a High Resolution Mass Spectrometry Procedure after Data Independent Acquisition

Desenvolvimento de método analítico envolvendo QuEChERS modificado e DLLME para a determinação de resíduos de eritromicina em peixe por LC-MS/MS

Contact Info

Product

Resources

About