Vorgehensweise und Hygienemaßnahmen beim Ausbruch von Herpes, Influenza, Druse oder Infektionen mit multiresistenten Keimen

Gehlen, Heidrun; Rutenberg, Dusty; Simon, Claudia; Reinhold-Fritzen, Birgitta; Drozdzewska, Karolina

doi:10.1055/a-1809-2163

Cited by 1 publication

(1 citation statement)

References 112 publications

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“…Since the specific needs and surroundings of hospitalized horses (bedding, boxes, floor surfaces, reviewed in (Gehlen et al, 2020) differ immensely from those of small animal or even human patients, the environment of equine clinics seems to play an important role in ESBL-EC accumulation and spread, including strains known for their pathogenic potential in humans and animals, i.e. isolates belonging to sequence type (ST)10 and ST410 (Kauter et al, 2021).…”

Section: Bacteroides Abundancesmentioning

confidence: 99%

Antibiotic prophylaxis and hospitalization of horses subjected to median laparotomy: gut microbiota trajectories and abundance increase ofEscherichia

Kauter

Brombach

Luebke-Becker

et al. 2023

Preprint

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Horse clinics are hotspots for the accumulation and spread of clinically relevant and zoonotic multidrug-resistant bacteria, including extended-spectrum β-lactamase producing (ESBL) Enterobacterales. Although median laparotomy in cases of acute equine colic is a frequently performed surgical intervention, knowledge about the effects of peri-operative antibiotic prophylaxis (PAP) based on a combination of penicillin and gentamicin on the gut microbiota is limited. Therefore, we collected fecal samples of horses from a non-hospitalized control group (CG) and from horses receiving either a pre-surgical single-shot (SSG) or a peri-operative 5-day (5DG) course of PAP. To assess differences between the two PAP regimens and the CG, all samples obtained at hospital admission (t0), on days three (t1) and ten (t2) after surgery, were screened for ESBL-producing Enterobacterales and subjected to 16S rRNA V1– V2 gene sequencing.We included 48 samples in the SSG (n=16 horses), 45 in the 5DG (n=15) and 20 in the CG (n=10). Two samples (6.5%) were positive for ESBL-producing Enterobacterales at t0while this rate increased to 67% at t1and decreased only slightly at t2(61%). Shannon diversity index (SDI) was used to evaluate alpha-diversity changes, revealing that horses suffering from acute colic seemed to have a compromised fecal microbiota composition (5DG, SDImeanof 5.90; SSG, SDImeanof 6.17) when compared to the CG (SDImeanof 6.53) at t0, although the difference lacked significance. Alpha-diversity decreased significantly in both PAP groups at t1, while at t2the onset of microbiome recovery was noticed. Although we did not identify a significant SDImeandifference with respect to PAP duration, the community structure (beta-diversity) was considerably restricted in samples of the 5DG at t1, most likely due to the ongoing administration of antibiotics. An increased abundance ofEnterobacteriaceae,especiallyEscherichia, was noted for both study groups at t1. Further studies are needed to reveal important factors promoting the increase and residency of ESBL-producing Enterobacterales among hospitalized horses.

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Section: Bacteroides Abundancesmentioning

confidence: 99%