Voluntary consumption of ethanol in 15 inbred mouse strains

Belknap, John K.; Crabbe, John C.; Young, Emmett R.

doi:10.1007/bf02244901

Cited by 525 publications

(470 citation statements)

References 32 publications

Supporting

Mentioning

388

Contrasting

Order By: Relevance

“…Across the 3 available data sets [4,21,22], the strain mean correlation between g/kg intake and preference ratio (the proportion of total daily fluid ingested from the ethanol bottle) was r = 0.88 across these 41 strains. Since we believe g/kg intake to be the superior index of avidity for ethanol solutions (as it depends less on the concentration of ethanol offered than does the preference ratio), we correlated the g/kg intake from the two-bottle tests with g/kg intake from the DID test, as well as the BEC after DID.…”

Section: Resultsmentioning

confidence: 99%

“…On the other hand, we had previously compared S13 HDID-1 and G64 HS mice for two bottle preference drinking using a protocol very similar to those employed for the inbred strain studies [4,21,22]. In that comparison, strains were offered 4 days each of choice between 3%, 6%, 9%, 12%, 15% ethanol versus water, and then higher concentrations [14].…”

Section: Discussionmentioning

confidence: 99%

“…Yoneyama [21] tested 22 inbred strains (8-10 weeks old) for 4 days each of 3%, then 6%, then 10% ethanol vs water. Belknap [22] used the same increasing series of ethanol concentrations but for only 3 days at each concentration in a test of 15 inbred strains aged 9-11 weeks. Bachmanov [4] tested 28 strains aged about 8 weeks for 4 days of 10% ethanol vs water.…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Ethanol Withdrawal-Associated Drinking and Drinking in the Dark: Common and Discrete Genetic Contributions

et al. 2012

View full text Add to dashboard Cite

Individual mice differ in the dose of ethanol they will ingest voluntarily when it is offered during limited access periods in the circadian dark, a phenotype called drinking in the dark (DID). Substantial genetic variation in DID has been reported across a few standard inbred mouse strains, and a line of High Drinking in the Dark (HDID) mice has been established through selective breeding on the blood ethanol concentration (BEC) they attain at the end of a drinking session. Here, we report ethanol DID data for 23 inbred mouse strains, including 11 not previously reported, corroborating the genetic contributions to this trait. We also report data on a different ethanol drinking trait, the increased intake seen after multiple cycles of chronic intermittent exposure to ethanol vapor (CIE). Drinking escalated significantly during ethanol withdrawal. However, HDID mice and their HS controls showed equivalent escalation during withdrawal, demonstrating that withdrawal-associated drinking escalation is not a clear genetic correlate of selection on DID. Across inbred strains, DID is substantially genetically correlated with previously-published two-bottle ethanol preference drinking data assessed under conditions of continuous ethanol access. Although inbred strain data for withdrawal-associated drinking are not available, the current pattern of results suggests that withdrawal-associated drinking is genetically distinct from DID, while genetic contributions to DID and two-bottle preference drinking are substantially similar.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Ethanol Withdrawal-Associated Drinking and Drinking in the Dark: Common and Discrete Genetic Contributions

et al. 2012

View full text Add to dashboard Cite

show abstract

“…C57BL/6J female mice were chosen for this experiment because they consistently consume higher amounts of alcohol and display higher levels of wheel running as compared with C57BL/6J males (Belknap et al, 1993;de Visser et al, 2007). Fifteen female mice were group housed three or four to a standard mouse cage (19.05 × 31.75 × 12 cm).…”

Section: Animals and Experimental Designmentioning

confidence: 99%

Wheel running, voluntary ethanol consumption, and hedonic substitution

Ozburn

Harris

Blednov

2008

Alcohol

View full text Add to dashboard Cite

Few studies have examined the relationship between naturally rewarding behaviors and ethanol drinking behaviors in mice. Although natural and drug reinforcers activate similar brain circuitry, there is behavioral evidence suggesting food and drug rewards differ in perceived value (Bickel et al., 1995;DeGrandpre et al., 1993;Petry and Heyman, 1995). The primary goal of the present study was to investigate the relationships between naturally reinforcing stimuli and consumption of ethanol in ethanol preferring C57BL/6J mice. Mouse behaviors were observed after the following environmental manipulations: standard or enhanced environment, accessible or inaccessible wheel, and presence or absence of ethanol. Using a high resolution volumetric drinking monitor and wheel running monitor, we evaluated whether alternating access to wheel running modified ethanol-related behaviors and whether alternating access to ethanol modified wheel running or subsequent ethanolrelated behaviors. We found that ethanol consumption remains stable with alternating periods of wheel running. Wheel running increases in the absence of ethanol and decreases upon reintroduction of ethanol. Upon reintroduction of ethanol, an alcohol deprivation effect was seen. Collectively, the results support theories of hedonic substitution and suggest that female C57BL/6J mice express ethanol seeking and craving under these specific conditions.

show abstract

“…For instance, protein kinase C gamma (PKCg)-null mutants from a C57BL/6J Â 129/SvJ mixed genetic background exhibited reduced ethanol sensitivity and the absence of ethanol tolerance, however, expression of the null mutation on a C57BL/6J background eliminated the phenotypes (Bowers 1999). Mice used in the present study were congenic C57BL/6J, which are widely known as a model of high alcohol preference (Belknap, 1993). Thus, it is plausible that background genes driving alcohol drinking in this mouse strain might blunt the effects of some null mutations, such as the 5-HT 3A receptor.…”

Section: -Ht 3 Antagonist-induced Reductions In Alcohol Drinkingmentioning

confidence: 93%

5-HT3A Receptor Subunit is Required for 5-HT3 Antagonist-Induced Reductions in Alcohol Drinking

Hodge

Kelley

Bratt³

et al. 2004

Neuropsychopharmacol

View full text Add to dashboard Cite

The ionotropic serotonin subtype-3 (5-HT 3 ) receptor has emerged as a potential therapeutic target in the treatment of alcohol abuse and alcoholism because selective pharmacological antagonists reduce alcohol consumption in preclinical and clinical models. 5-HT binds to the extracellular N-terminus of the 5-HT 3A receptor subunit but receptor activation is also enhanced by distinct allosteric sites, which indicates the presence of other receptor subunits. It is not known if specific molecular subunits of the 5-HT 3 receptor modulate alcohol drinking. To address this issue, we characterized acute locomotor response to alcohol and alcohol consumption in a two-bottle homecage procedure by congenic C57BL/6J mice with a targeted deletion of the 5-HT 3A receptor subunit gene. 5-HT 3A -null mice did not differ from wild-type littermate controls on measures of spontaneous locomotor activity, habituation to a novel environment, or locomotor response to ethanol (0, 0.5, 1, or 2 g/kg). Moreover, null mice did not differ from controls on measures of ethanol (2-10%) intake and preference during or after a two-bottle home-cage sucrose fading procedure. Systemic administration of the 5-HT 3 antagonist LY-278,584 (0-10 mg/kg) decreased intake of both sweetened (2% sucrose þ 10% ethanol) and unsweetened (10% ethanol) ethanol in wild-type mice only. These findings indicate that reduction of alcohol drinking produced by 5-HT 3 antagonism is dependent on the presence of 5-HT 3A -containing receptors.

show abstract

Voluntary consumption of ethanol in 15 inbred mouse strains

Cited by 525 publications

References 32 publications

Ethanol Withdrawal-Associated Drinking and Drinking in the Dark: Common and Discrete Genetic Contributions

Ethanol Withdrawal-Associated Drinking and Drinking in the Dark: Common and Discrete Genetic Contributions

Wheel running, voluntary ethanol consumption, and hedonic substitution

5-HT3A Receptor Subunit is Required for 5-HT3 Antagonist-Induced Reductions in Alcohol Drinking

Contact Info

Product

Resources

About