Volume regulation following hyposmotic shock in isolated turbot (Scophthalmus maximus) hepatocytes

Ollivier, Hélène; Pichavant, K.; Puill-Stephan, Eneour; Roy, Stella; Calvés, Patrick; Nonnotte, Liliane; Nonnotte, G.

doi:10.1007/s00360-005-0061-8

Cited by 21 publications

(23 citation statements)

References 30 publications

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“…We also considered that this species is optionally anadromous (Hoar 1988) and thus potentially exposed to large fluctuations in external salinity. In addition, despite reports concerning cell volume regulation in several species of fish, including trout (Kanli and Norderhus 1998;Egee et al 2000;Guizouarn et al 2000;Leguen and Prunet 2004;Espelt et al 2008), turbot (Ollivier et al 2006), eel (Lionetto et al 2005;Trischitta et al 2005), catfish (Goswami and Saha 2006), and little skate (Puffer et al 2006), there is a surprising paucity of data regarding RVD in salmon. Accordingly, the purpose of this study was to investigate the cellular basis by which salmon RBCs regulate volume under hypotonic conditions.…”

Section: Introductionmentioning

confidence: 87%

“…, we conducted a preliminary examination to determine whether volume recovery depended on the Ca 2? -activated enzyme phospholipase A 2 (PLA 2 ), which catalyzes the synthesis of arachidonic acid (AA, Jenkins et al 2009;Ollivier et al 2006). This was accomplished using the PLA 2 antagonist ON-RS-082 (10 lM, Doody et al 2009).…”

Section: Cell Volume Studiesmentioning

confidence: 99%

“…Although the cellular mechanisms that activate and regulate permeability pathways during RVD are not completely understood and differ between cell types (Wehner et al 2003;Espelt et al 2008;Hoffmann et al 2009), Ca 2? has been shown to play a key role in a number of cases (Foskett 1994; Pasantes-Morales and Morales 2000; Tinel et al 2000;Leguen and Prunet 2004;Ollivier et al 2006).…”

Section: Introductionmentioning

confidence: 98%

“…response is mediated by Ca 2? release from intracellular stores (Foskett 1994;Tinel et al 2000;Leguen and Prunet 2004;Lionetto et al 2005;Trischitta et al 2005;Ollivier et al 2006). In addition, although Ca 2?…”

Section: Introductionmentioning

confidence: 99%

“…may directly activate ion channels during RVD (Foskett 1994;Lang 2007;PasantesMorales and Morales 2000), there is evidence that several Ca 2? -dependent intracellular messengers and enzymes (e.g., calmodulin, phospholipase A 2 , eicosanoids, and protein kinases) are involved with cell volume regulation (Tinel et al 2000;Ebner et al 2006;Ollivier et al 2006;Hoffmann et al 2009). Furthermore, the cytoskeleton is thought to play a role in volume regulation, and actin polymerization is dependent on intracellular Ca 2?…”

Section: Introductionmentioning

confidence: 99%

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Regulatory volume response following hypotonic stress in Atlantic salmon erythrocytes

Wormser

Mason

Helm

et al. 2011

Fish Physiol Biochem

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The purpose of this study was to examine regulatory volume decrease (RVD) in Atlantic salmon red blood cells (RBCs). Osmotic fragility was determined optically, mean cell volume was measured electronically, and changes in intracellular Ca(2+) concentration were visualized using fluorescence microscopy and fluo-4-AM. Cells displayed an increase in osmotic fragility and an inhibition of volume recovery following hypotonic shock when they were exposed to a high taurine Ringer or when placed in a high K(+) medium. Interestingly, RVD in cells from fish collected during the summer depended more on taurine efflux, whereas fall cells relied more on the loss of K(+). In addition, RVD in fall cells was prevented with the K(+) channel inhibitor quinine, whereas the ionophore gramicidin decreased osmotic fragility and potentiated volume recovery. Further, hypotonic shock (0.5X Ringer) for both summer and fall cells caused an increase in cytosolic Ca(2+), which resulted from influx of this ion because it was not observed when extracellular Ca(2+) was chelated with EGTA (10 nM free Ca(2+)). Cells exposed to a low Ca(2+) hypotonic Ringer also had a greater osmotic fragility and failed to recover from hypotonic swelling. Finally, inhibition of phospholipase A(2) with ONO-RS-082 blocked volume recovery. In conclusion, Atlantic salmon RBCs displayed volume decrease in response to hypotonic shock, which depended on a swelling-induced influx of Ca(2+) and an increase in the efflux of K(+) and taurine.

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Section: Introductionmentioning

confidence: 87%

Section: Cell Volume Studiesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Regulatory volume response following hypotonic stress in Atlantic salmon erythrocytes

Wormser

Mason

Helm

et al. 2011

Fish Physiol Biochem

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Digestive cells from Mytilus galloprovincialis show a partial regulatory volume decrease following acute hypotonic stress through mechanisms involving inorganic ions

Torre

Trischitta

Corsaro

et al. 2012

Cell Biochemistry & Function

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The response of isolated digestive cells of the digestive gland of Mytilus galloprovincialis to hypotonic shock was studied using videometric methods. The isolated cells exposed to a rapid change (from 1100 to 800 mosmol kg(-1) ) of the bathing solution osmolality swelled but thereafter underwent a regulatory volume decrease (RVD), tending to recover the original size. When the hypotonic stress was applied in the presence of quinine and glibenclamide, known inhibitors of swelling activated ion channels, the cells did not exhibit an RVD response; in addition, they showed a larger increase in size in respect to control cells. These observations suggest that the digestive cells of the digestive gland have the machinery to cope with the hyposmotic shock allowing them to exhibit a small but significant RVD preventing an excessive increase in cell size. The pharmacological treatment of digestive cells during the RVD experiments suggests that cell volume is regulated by K(+) and Cl(-) efflux followed by an obliged water efflux from the cell. The involvement of organic osmolytes such as taurine and betaine seems to be excluded by NMR measurement on digestive cells.

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Desiccation Resistance in Embryos of the Killifish, Fundulus heteroclitus: Single Embryo Measurements

2013

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Northern killifish, Fundulus heteroclitus macrolepidotus, spawn in estuaries at high tides. Embryos may be stranded in air at stream margins as the water recedes. These aerially incubated embryos are exposed to desiccation stress and may survive and develop normally to hatching at ∼14 days post-fertilization (dpf). We developed a technique to quantitatively measure the kinetics of water loss at various developmental stages from single embryos in controlled relative humidities (RHs). Embryos were able to tolerate short periods (2 hr) of severe desiccation and survive to hatching. Mid-stage (7 dpf) embryos showed the highest degree of desiccation tolerance compared to early-stage (2 dpf) and late-stage (14 dpf) embryos. We classified the patterns of water loss into four phases, the perivitelline space (PVS) phase, the resistance phase, the desiccation phase, and the equilibration phase. In the PVS phase, water loss was rapid at all developmental stages and all RHs (∼25% of total embryo weight). The water loss rate was slower during the resistance phase. It decreased as RH increased and length of this phase was longer in mid-stage than in early- and late-stage embryos. The water loss rate and length of the desiccation phase also depended on RH. These data support the hypothesis that low permeability embryonic compartment surface membranes retard water loss significantly and promote prolonged survival of these embryos during desiccation. We also show this mechanism cannot completely account for the survival of severely desiccated embryos (especially in 23% RH) and that there must also be complementary cellular responses.

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Volume regulation following hyposmotic shock in isolated turbot (Scophthalmus maximus) hepatocytes

Cited by 21 publications

References 30 publications

Regulatory volume response following hypotonic stress in Atlantic salmon erythrocytes

Regulatory volume response following hypotonic stress in Atlantic salmon erythrocytes

Digestive cells from Mytilus galloprovincialis show a partial regulatory volume decrease following acute hypotonic stress through mechanisms involving inorganic ions

Desiccation Resistance in Embryos of the Killifish, Fundulus heteroclitus: Single Embryo Measurements

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