Volume regulation by human lymphocytes. Identification of differences between the two major lymphocyte subpopulations.

Cheung, Roy K.; Grinstein, Sergio; Gelfand, Erwin W.

doi:10.1172/jci110657

Cited by 56 publications

(23 citation statements)

References 20 publications

(29 reference statements)

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“…T lymphocytes were obtained following E-rosette enrichment as described (Cheung et al, 1982a). Greater than 95% of the cells obtained in this way were identified as T lymphocytes.…”

Section: Methods Cellsmentioning

confidence: 99%

Role of membrane potential in the regulation of lectin‐induced calcium uptake

Gelfand

Cheung

Grinstein

1984

Journal Cellular Physiology

114

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Incubation of lymphocytes with mitogenic lectins triggers Ca2+ uptake. This increase in free cytoplasmic Ca2+ is postulated to be an important signal in the initiation of DNA synthesis. Transmembrane fluxes of monovalent ions and changes in membrane potential are also associated with lectin-induced activation of lymphocytes. We have examined the relationship between extra-cellular monovalent ion substitution, the associated electrical potential changes (measured with cyanine dyes), phytohemagglutinin-induced Ca2+ uptake (measured with Quin-2) and proliferation in human T cells. The results show that (1) the magnitude of the increase in free cytoplasmic Ca2+ concentration is correlated with the extent of the lymphoproliferative response, (2) lectin-induced Ca2+ fluxes are sensitive to membrane potential, decreasing with depolarization, and are likely conductive, and (3) the presence of extra-cellular Na+ during incubation with phytohemagglutinin is not essential to mitogenic triggering.

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“…T lymphocytes were obtained following E-rosette enrichment as described (Cheung et al, 1982a). Greater than 95% of the cells obtained in this way were identified as T lymphocytes.…”

Section: Methods Cellsmentioning

confidence: 99%

Role of membrane potential in the regulation of lectin‐induced calcium uptake

Gelfand

Cheung

Grinstein

1984

Journal Cellular Physiology

114

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“…1977;Cheung et al. 1982a;Deutsch et al, 1982;Bui and Wiley, 1981;Doljanski et al, 1974;Ben-Sasson et al, 1975;Lee et al, 1988;Gelfand et al, 1984], whereas lymphocytes of the B cell lineage exhibit minimal RVD [Cheung et al, 1982b;Gelfand et al, 1984], Primary murine peripheral T cells and mu rine T cell clones in a nonproliferating state express low levels of K+ channels McKinnon and Ceredig, 1986;De Coursey et al, 1987] and do not exhibit RVD [Leeetal.. 1988]. However, voltage-gated K+ conductance is increased in these cells during cell cycle progression DeCoursey et al, 1987], enabling these cells to regulate volume in response to hvpotonicity [Lee et al, 1988] (see below).…”

Section: Mechanism Of Rvdmentioning

confidence: 99%

Cell Volume Regulation in Lymphocytes

Deutsch¹,

Lee²

1988

Kidney Blood Press Res

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This article reviews what is known about the volume regulatory responses of lymphocytes. We present a discussion of recent data and hypotheses pertaining to the underlying mechanisms in regulatory volume increase (RVI) and regulatory volume decrease (RVD). New results from our laboratory are included to demonstrate that RVD is modulated by both temperature and pH, and that RVD occurs in proliferating as well as quiescent lymphocytes. This information is considered in the context of a model that includes the dynamics of membrane potential, K⁺ conductance, Cl^- conductance, a proposed stretch-activated conductance, gating mechanisms, and equilibrium potentials, as RVD progresses. The physiological relevance of volume homeostasis in lymphocyte function, in particular, and in cell growth and proliferation, in general, is discussed.

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“…These inhibitors do not block the maxi-anion channel. However, they block the volume-sensitive outwardly rectifying Cl -channel [4]. Probably, this channel plays an important role in the permeability of thymocytes for anions during hypotonic stimulation.…”

Section: Resultsmentioning

confidence: 99%

“…They swell rapidly, but shrink slowly in the follow-up period. These changes are mediated by the system for regulatory volume decrease [4]. Immature lymphocytes of the thymus (thymocytes) rapidly proliferate and are subjected to a complex process of positive or negative selection.…”

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confidence: 99%