Volume of RBCs, 24‐ and 48‐hour posttransfusion survivals, and the lifespan of ⁵¹Cr and biotin‐X‐N‐hydroxysuccinimide (NHS)‐labeled autologous baboon RBCs: effect of the anticoagulant and blood pH on ⁵¹Cr and biotin‐X‐NHS elution in vivo

Abstract

The anticoagulant and the pH of the medium in which the RBCs were labeled with (51)Cr or biotin-X-NHS affected in vivo elution of the label from the RBCs and may reduce posttransfusion survival values.

Comparison of radioisotope methods and a nonradioisotope method to measure the RBC volume and RBC survival in the baboon

“…Our recent observation in baboons transfused with autologous fresh RBCs labeled with 51 Cr and biotin‐X‐NHS demonstrated that the lesion of collection reported by Gibson and associates 32,33 was due to 51 Cr elution from the fresh RBCs and not to the removal of nonviable 51 Cr‐labeled autologous RBCs 23 . When the pH value of the medium in which the RBCs are labeled is 6.0 or lower, the rate of elution of 51 Cr from the RBCs is increased 23 . The elution of 51 Cr, 99m Tc, and 111 In‐oxine from autologous liquid preserved RBCs reported by Marcus and associates 12 may have been affected by the pH of the RBCs at the time of labeling.…”

“…Poorly preserved autologous RBCs are removed from the circulation during the mixing time and will not be detected with the extrapolated 51 Cr‐labeled RBC radioactivity during the immediate posttransfusion period 39 . The 51 Cr label elutes at a rapid rate during the 24‐ to 48‐hour posttransfusion period and during the life span when the RBCs are labeled with 51 Cr in a medium in which the pH value is 6.0 or lower 23 . The increased rate of 51 Cr elution will reduce the 51 Cr 24‐hour posttransfusion survival and life span of the autologous preserved RBCs 23 .…”

“…The 51 Cr label elutes at a rapid rate during the 24‐ to 48‐hour posttransfusion period and during the life span when the RBCs are labeled with 51 Cr in a medium in which the pH value is 6.0 or lower 23 . The increased rate of 51 Cr elution will reduce the 51 Cr 24‐hour posttransfusion survival and life span of the autologous preserved RBCs 23 . This finding requires that 51 Cr labeling of the preserved autologous RBCs should be done in a medium with a pH of greater than 6.0 23 …”

“…The increased rate of 51 Cr elution will reduce the 51 Cr 24‐hour posttransfusion survival and life span of the autologous preserved RBCs 23 . This finding requires that 51 Cr labeling of the preserved autologous RBCs should be done in a medium with a pH of greater than 6.0 23 …”

“…Additional blood samples were collected 24 and 48 hours after autotransfusion and weekly until the level of biotin‐positive RBCs in the recipient reached 0.5 percent, the lowest level detectable with the flow cytometer and fluorescent streptavidin. The RBC volume, the 24‐hour posttransfusion survival, and the life span of the autologous RBCs were measured in the biotin‐X‐NHS labeled RBCs detected in a flow cytometer (Epics XL, Coulter Corp., Hialeah, FL) with fluorescent streptavidin 23 …”