Volume expansion of erythrocytes is not the only mechanism responsible for the protection by arginine-based surfactants against hypotonic hemolysis

Fait, María Elisa; Hermet, Melisa; Vázquez, Romina Florencia; Maté, Sabina María; Millone, M. Antonieta Daza; Vela, M. E.; Morcelle, Susana R.; Bakás, Laura Susana

doi:10.1016/j.colsurfb.2018.07.005

Cited by 9 publications

(5 citation statements)

References 25 publications

(36 reference statements)

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“…This protective effect is often observed at surfactant concentrations below the corresponding CMC value. However, our previous published work demonstrated that Bz-Arg-NHC n display both hemolytic and protective activity above this parameter (Fait et al 2018). Moreover, Joondan and coworkers proved a similar trend for the hemolytic activity of aromatic amino acid-based surfactants, evidencing the crucial role of the surfactants' aggregates in the disruption of the membrane structure (Joondan et al 2016(Joondan et al , 2017Fait et al 2018).…”

Section: Discussionmentioning

confidence: 84%

Interaction of cationic surfactants with DPPC membranes: effect of a novel Nα-benzoylated arginine-based compound

et al. 2021

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Cationic amino acid-based surfactants are known to interact with the lipid bilayer of microorganism resulting in cell death through a disruption of the membrane topology. To elucidate the interaction of a cationic surfactant synthesized in our lab, investigations involving N α -benzoyl-arginine decyl amide (Bz-Arg-NHC 10 ), and model membranes composed by 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) were done. Bz-Arg-NHC 10 was able to penetrate into DPPC monolayers up to a critical pressure of 59.6 mN m −1 . Differential scanning calorimetry revealed that as the concentration of Bz-Arg-NHC 10 increased, the main transition temperature of DPPC slightly decreased. Atomic force microscopy (AFM) in situ experiments performed on supported DPPC bilayers on mica allowed monitoring the changes induced by Bz-Arg-NHC 10 . DPPC bilayer patches were partially removed, mainly in borders and bilayer defects for 50 µM Bz-Arg-NHC 10 solution. Increasing the concentration to 100 µM resulted in a complete depletion of the supported bilayers. Surface plasmon resonance (SPR) experiments, carried out with fully DPPC bilayers covered chips, showed a net increase of the SPR signal, which can be explained by Bz-Arg-NHC 10 adsorption. When patchy DPPC bilayers were formed on the substrate, a SPR signal net decrease was obtained, which is consistent with the phospholipids' removal observed in the AFM images. The results obtained suggest that the presence of the benzoyl group attached to the polar head of our compound would be the responsible of the increased antimicrobial activity against gram-negative bacteria when compared with other arginine-based surfactants.

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Section: Discussionmentioning

confidence: 84%

Interaction of cationic surfactants with DPPC membranes: effect of a novel Nα-benzoylated arginine-based compound

et al. 2021

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“…Those authors reported that the QAS derivatives induced a signi cant cell-wall thickening as well as membrane deformations along with lipiddroplet formation in the cytoplasm, acting also as oxidative-stress stimulants. Bz-Arg-NHC n was previously found to induce both membrane permeabilization and induction of oxidative stress as part of their antifungal mechanisms (Fait et al 2018). The combination of these effects may also alter the cell wall, as evidenced in the cell-roughness and stiffness changes in the Bz-Arg-NHC n -treated yeasts.…”

Section: Antibio Lm Assaysmentioning

confidence: 99%

Candidicidal Effect of Arginine-based Surfactants

Fait¹,

Grillo²,

Garrote

et al. 2023

Preprint

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Amino-acid–based surfactants are a group of compounds that resemble natural amphiphiles and thus are expected to have a low impact on the environment, owing to either the mode of surfactant production or its means of disposal. Within this context, arginine-based tensioactives have gained particular interest since their cationic nature—in combination with their amphiphilic character—enables them to act as broad-spectrum biocides. This capability is based mainly on their interactive affinity for the microbial envelope that alters the latter’s structure and ultimately its function. In the work reported here, we investigated the efficiency of Nα-benzoyl arginine decyl- and dodecylamide against Candida spp. to further our understanding of the antifungal mechanism involved. For the assays, both a Candida albicans and a Candida tropicalis clinical isolates along with a C. albicans–collection strain were used as references. As expected, both arginine-based compounds proved to be effective against the strains tested through inhibiting both the planktonic and the sessile growth. Furthermore, atomic-force–microscopy techniques and lipid-monolayer experiments enabled us to gain insight into the effect of the surfactant on the cellular envelope. The results demonstrated that all the yeasts treated exhibited changes in their exomorphologic structure, with respect to alterations in both roughness and stiffness, relative to the nontreated ones. This finding—in addition to the amphiphiles’ proven ability to insert themselves within this model fungal membrane—could explain the changes in the yeast-membrane permeability that could be linked to viability loss and mixed-vesicle release.

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“…However, the boundary between those two mechanisms is not clearly defined and may occur simultaneously. 45,47 It was assumed that if hemolysis took place through the osmotic hemolysis mechanism, then it could be protected by solutes with appropriate sizes. 46 In this work, to determine the hemolysis mechanism of PL-C n -x and the size of the resulting pores on the hRBC membrane, an osmotic protectant experiment was performed.…”

Section: Hemolysis and Cytotoxicity Of Pl-c N -Xmentioning

confidence: 99%

Facilitating trehalose entry into hRBCs at 4 °C by alkylated ε-poly(l-lysine) for glycerol-free cryopreservation

et al. 2022

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Volume expansion of erythrocytes is not the only mechanism responsible for the protection by arginine-based surfactants against hypotonic hemolysis

Abstract: Highlights Arginine-based surfactant Bz-Arg-NHC12 has both hemolytic and antihemolytic effects.  Both processes depend on the lipid composition of the erythrocyte membrane.  SRBC is more resistant than HRBC to the hemolytic effect of the surfactant.

Cited by 9 publications

References 25 publications

Interaction of cationic surfactants with DPPC membranes: effect of a novel Nα-benzoylated arginine-based compound

Interaction of cationic surfactants with DPPC membranes: effect of a novel Nα-benzoylated arginine-based compound

Candidicidal Effect of Arginine-based Surfactants

Facilitating trehalose entry into hRBCs at 4 °C by alkylated ε-poly(l-lysine) for glycerol-free cryopreservation

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