Volume changes of tissues in physiological fluids during fixation in osmium tetroxide or formaldehyde and during subsequent treatment

Bahr, G. F.; Bloom, Gunnar D.; Friberg, Ulla

doi:10.1016/0014-4827(57)90148-9

Cited by 182 publications

(55 citation statements)

References 18 publications

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“…The fixation in formalin, dehydration in ethanol, and embedding in paraffin may contribute to a tissue shrinkage of 30% to 40%. 13 A few recent studies on the validation of ultrasound-determined IMT carried out in vitro and/or in situ had reported a much lower shrinkage of only 2.5% to 14.6%. 4 -6 However, in the study by Zarins et al 14 involving 10 human superficial femoral arteries, the vessel wall decreased by 36% because of postmortem shrinkage, which is in accordance with the present study, establishing a mean difference of Ϫ31% (Ϫ0.34 mm) between ultrasound and histological findings.…”

Section: Discussionmentioning

confidence: 99%

Accuracy of In Vivo Carotid B-Mode Ultrasound Compared With Pathological Analysis

Schulte-Altedorneburg

Droste

Felszeghy

et al. 2001

Stroke

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Background and Purpose-This study aimed to determine the correlation of in vivo ultrasound measurements of intima-media thickening (IMT), lumen diameter, and cross-sectional area of the common carotid artery (CCA) with corresponding measurements obtained by gross pathology and histology. Methods-Sixty-six moribund neurological patients (mean age 71 years) underwent B-mode ultrasound of the CCA a few days before death. During autopsy, carotid specimens were removed in toto. Carotid arteries were ligated and cannulated for injection of a hydrophilic embedding material under standardized conditions. The carotid bifurcation was frozen and cut manually in 3-mm cross slices. Digital image analysis was carried out to determine the diameter and the cross-sectional area of the frozen slices of the CCA. IMT was assessed by light microscope. Ultrasonic and planimetric data were compared. Results-Mean measurements of lumen diameter and cross-sectional area were 7.13Ϯ1.27 mm and 0.496Ϯ0.167 cm 2 , respectively, by ultrasound, and 7.81Ϯ1.45 mm and 0.516Ϯ0.194 cm 2 , respectively, by planimetric analysis of the unfixed redistended carotid arteries (R 2 ϭ0.389 and 0.497). The mean IMT was 1.005Ϯ0.267 mm by ultrasound and 0.67Ϯ0.141 mm histologically, resulting in a mean difference of Ϫ31%. Conclusions-Transcutaneous

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Section: Discussionmentioning

confidence: 99%

Accuracy of In Vivo Carotid B-Mode Ultrasound Compared With Pathological Analysis

Schulte-Altedorneburg

Droste

Felszeghy

et al. 2001

Stroke

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“…Conversely, in relatively rigid endarterectomy specimens which hold a high proportion of minerals, no significant shrinkage is expected. In our view, the distortion in measured values is quite low, and does not fall under the current literature classification related to postoperative perfusion of blood vessels [8][9][10][11] .…”

Section: Discussionmentioning

confidence: 39%

Postoperative determination of the degree of carotid artery stenosis

Janzen¹,

Chenais²,

Janzen³

et al. 2013

J. vasc. bras.

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This article describes the VascMorph 1a prototype software and reports first results obtained with postoperative determination of the degree of stenosis in the carotid artery.Keywords: degree of stenosis; atherosclerosis; carotid artery. ResumoEste artigo descreve o programa protótipo VascMorph 1a e apresenta os primeiros resultados obtidos com a determinação pós-operatória do grau de estenose na artéria carótida. This is a reproduction in English of the article originally published in German, entitled "Postoperative Stenosegradbestimmung der atherosklerotischen Arteria carotis mit VascMorph 1a" (Perfusion. 2013;26:8-11). Permission for reproduction has been obtained from Verlag Perfusion GmbH. PalavrasPostoperative determination of the degree of carotid artery stenosis 130

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“…Particularly important in the initial stages are the tonicity of the dilutant and the tissue fluids, membrane permeability, Donnan " iii equilibria, and inability of the cells to continue active osmotic work (Bahr et al 1957). Over a longer period, slow chemical changes may also have an effect.…”

Section: Discussionmentioning

confidence: 99%

“…Weight loss could also result from breakdown and extraction of relatively dense tissue components such as proteins or glycogen. Bahr et al (1957) noted that, although individual data varied greatly, most previous investigations have revealed that fixation in formaldehyde caused tissue samples to swell followed by a gradual decrease in volume and weight.…”

Section: Time In 10% Formalin (Days)mentioning

confidence: 99%

Effect of storage in formalin on organ weights of rabbits

Fraser

1985

New Zealand Journal of Zoology

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The changes in weight of liver, kidneys, perirenal fat, adrenals, ovaries, and testes from a sample of 37 rabbits, Oryctolagus cuniculus (L.), following storage for up to 128 days in 10% formalin are described. The greatest changes caused by preservation occur in the first 24 h, but continue at a decreasing rate until by Day 32 only minor differences were found between successive weighings. In a large volume of formalin all tissue maintained greater weight than similar tissue stored in a small volume. Correction factors are necessary to estimate initial weights from material preserved in 10% formalin.

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Volume changes of tissues in physiological fluids during fixation in osmium tetroxide or formaldehyde and during subsequent treatment

Cited by 182 publications

References 18 publications

Accuracy of In Vivo Carotid B-Mode Ultrasound Compared With Pathological Analysis

Accuracy of In Vivo Carotid B-Mode Ultrasound Compared With Pathological Analysis

Postoperative determination of the degree of carotid artery stenosis

Effect of storage in formalin on organ weights of rabbits

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