Voltammetry and Spectroscopy Study of In Vitro Interaction of Fenitrothion with DNA

Ahmadi, Farhad; Jafari, Batool

doi:10.1002/elan.201000516

Cited by 34 publications

(3 citation statements)

References 53 publications

(48 reference statements)

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“…It has been reported that intercalators reduce EB‐DNA fluorescence by more than 50%, whereas the [compound]/[DNA] ratio ( r ) is below 100. [ 24,25 ] In this study, as the r increased from 0.028 to 0.2, and the EB‐DNA fluorescence decreased by approximately 75% for the compounds (Figure 8c and Table 1). These results indicate that BDPY‐4 and BDPY‐5 bind to DNA mainly through intercalative binding mode.…”

Section: Resultsmentioning

confidence: 58%

Synthesis of water‐soluble BODIPY dyes and investigation of their DNA interaction properties and cytotoxicity/phototoxicity

Keleş

Barut

Yıldırım

et al. 2021

Applied Organom Chemis

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In this study, water‐soluble BODIPY dyes (BDPY‐4, BDPY‐5) bearing dimethylamino and diethylamino groups were synthesized for the first time. The DNA interaction and nuclease effect of two water‐soluble BODIPY dyes were examined using absorption, competitive binding experiments, and agarose gel electrophoresis. Then, cytotoxicity and phototoxicity of the compounds were determined against A549 cells by MTT cell viability assay. The ultraviolet–visible (UV–vis) spectra of BDPY‐4 and BDPY‐5 displayed hypochromism and absorption maxima shifted to the red region. In addition, Kb values of the compounds were calculated as 2.23 × 104 and 4.84 × 104 M−1, respectively. An high‐performance liquid chromatography (HPLC) instrument without a column was used to conduct competitive binding experiments for the first time. The approach prevented the decrease in the response due to dilution and enabled the analysis to be carried out in a short time. Upon increasing concentrations of the compounds, the emission intensity of EB‐DNA significantly decreased. These obtained results suggested that the compounds bound to CT‐DNA with intercalative mode. At the same time, the Stern–Volmer constants were determined as 1.80 × 106 and 1.79 × 106 M−1 for BDPY‐4 and BDPY‐5, respectively, which is coherent with intercalative binding mode. The compounds did not display noticeable photonuclease actions with light irradiation. BDPY‐4 and BDPY‐5 showed moderate phototoxic properties on light irradiation.

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Section: Resultsmentioning

confidence: 58%

Synthesis of water‐soluble BODIPY dyes and investigation of their DNA interaction properties and cytotoxicity/phototoxicity

Keleş

Barut

Yıldırım

et al. 2021

Applied Organom Chemis

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“…The negative value of the change in free energy of binding indicated the spontaneous nature of the process, hence suggests the toxic nature of the compound. However, a much smaller value as compared to other insecticides as reported in literature, [50–52] indicates lesser probability of the occurrence of the DNA‐PYR intercalation process and lesser toxicity as compared to conventional insecticides. Therefore, it can be concluded that PYR can be carcinogenic towards human if exposed at higher concentrations.…”

Section: Binding Studiesmentioning

confidence: 78%

Density Functional Theory, Spectroscopic and Receptor Binding Analysis of Pyriproxyfen – A Commercial Insect Growth Regulator

Kumari,

Devi,

Sharma

et al. 2023

ChemistrySelect

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In the present study, the spectroscopic investigation of pyriproxyfen (PYR) has been carried out using density functional theory and experimental methods. The FT‐IR, 1H and 13C NMR spectra of PYR were recorded, interpreted and compared with the theoretical spectra. The polarizability and hyperpolarizabilities are responsible for higher lipophilicity which leads to the acute aquatic toxicity of the PYR molecule. The UV‐Visible spectra of PYR exhibited the presence of π π* transition between the π‐orbitals of phenoxyphenyl and pyridyl ring. The standard entropy, heat capacity at constant volume and total energy have been computed to assess the toxicity of the compound. Molecular docking revealed the insecticidal potential of PYR owing to the presence of hydrogen bond interactions between PYR and mosquito juvenile hormone binding protein of aedes aegypti. The biophysical study predicts the possible DNA‐PYR interactions and carcinogenic properties of PYR molecule.

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“…-60C20 complex in different solvent mixtures was calculated by fitting the observed chemical shifts at various M 2? / 60C20 mol ratios with a nonlinear least-squares program in MATLAB (version 7.8) [22].…”

Section: Stability Constants Calculationsmentioning

confidence: 99%

NMR study of the stoichiometry and stability of complexation reaction between Mg2+, Ca2+, Sr2+ and Ba2+ ions and 60-crown-20 in binary acetonitrile solvent mixtures

Rahimi‐Nasrabadi

Pourmohamadian

Pourmortazavi

et al. 2011

J Incl Phenom Macrocycl Chem

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Proton nuclear magnetic resonance ( 1 H-NMR) spectroscopy was used to study the complexation reaction between Mg 2? , Ca 2? , Sr 2? and Ba 2? ions and 60-crown-20 in a series of binary mixtures of deuterated acetonitrile (AN), nitromethane (NM) and D 2 O at 27°C. Formation constants of the 1:1 complexes were determined through computer fitting of the chemical shift/mol ratio data and found to vary in the order of Ba 2? [ Sr 2? [ Mg 2? & Ca 2? . The influence of the solvent composition on the stability of the resulting complexes was also discussed. In all cases, the changes in the stability constants with the solvent composition were monotonic and showed a good correlation with the inherent solvation ability of the pure solvents which form the mixture.

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Voltammetry and Spectroscopy Study of In Vitro Interaction of Fenitrothion with DNA

Cited by 34 publications

References 53 publications

Synthesis of water‐soluble BODIPY dyes and investigation of their DNA interaction properties and cytotoxicity/phototoxicity

Synthesis of water‐soluble BODIPY dyes and investigation of their DNA interaction properties and cytotoxicity/phototoxicity

Density Functional Theory, Spectroscopic and Receptor Binding Analysis of Pyriproxyfen – A Commercial Insect Growth Regulator

NMR study of the stoichiometry and stability of complexation reaction between Mg2+, Ca2+, Sr2+ and Ba2+ ions and 60-crown-20 in binary acetonitrile solvent mixtures

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