Voltammetric Sensor with Replaceable Polyaniline‐DNA Layer for Doxorubicin Determination

A DNA sensor has been proposed on the platform of glassy carbon electrode modified with native DNA implemented between two electropolymerized layers of polyaniline. The surface layer was assembled by consecutive stages of potentiodynamic electrolysis, DNA drop casting, and second electrolysis, which was required for capsulation of the DNA molecules and prevented their leaching into the solution. Surface layer assembling was controlled by cyclic voltammetry, electrochemical impedance spectroscopy, atomic force, and scanning electron microscopy. For doxorubicin measurement, the DNA sensor was first incubated in the Methylene blue solution that amplified signal due to DNA intercalation and competition with the doxorubicin molecules for the DNA binding sites. The charge transfer resistance of the inner layer interface decreased with the doxorubicin concentration in the range from 1.0 pM to 0.1 μM (LOD 0.6 pM). The DNA sensor was tested for the analysis of spiked artificial urine samples and showed satisfactory recovery in concentration range of 0.05–10 μM. The DNA sensor developed can find application in testing of antitumor drugs and some other DNA damaging factors.

Section: Resultsmentioning

confidence: 99%

Electrochemical DNA Sensors with Layered Polyaniline—DNA Coating for Detection of Specific DNA Interactions

Kulikova

Porfireva

Evtugyn

et al. 2019

“…Recently [25,26], we have shown that DNA could be used in a biosensor assembly as a collector of doxorubicin. Their interaction results in intercalation of the DNA helix followed by changes in its charge and volume.…”

Section: Resultsmentioning

confidence: 99%

“…Similar behavior exerts polypyrrole, though its response to biochemical reactions with DNA is milder than that of polyaniline [22]. Appropriate DNA sensors were reported for the determination of anthracyclines [23,24,25,26], salicylates [22], isoproterenol [27], and phenothiazine drugs [18,28]. However, electroconductivity of polyaniline is observed in strongly acidic conditions.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Sensor Based on Poly(Azure B)-DNA Composite for Doxorubicin Determination

Porfireva

Vorobev

Babkina

et al. 2019

Self Cite

A new voltammetric DNA sensor has been developed for doxorubicin determination on the platform of a glassy carbon electrode (GCE) covered with electropolymerized Azure B film and physically adsorbed native DNA. The redox properties of polymeric Azure B were monitored at various pH and scan rates. DNA application decreased the peak currents related to polymeric and monomeric forms of the dye, whereas incubation in doxorubicin solution partially restored the peaks in accordance with the drug and DNA concentration. The relative shift of the cathodic peak current caused by doxorubicin depended on the nominal DNA concentration and its application mode. In optimal conditions, the DNA sensor makes it possible to determine between 0.1 μM to 0.1 nM doxorubicin (limit of detection 7 × 10−11 M). The DNA sensor was tested on commercial doxorubicin formulations and on artificial samples the mimicked electrolyte content of human serum.

“…Accumulation of double-stranded DNA on electropolymerized Neutral red resulted in detection of 0.1 nM doxorubicin by changes in the R et values in the presence of ferricyanide ions [51]. The use of DNA entrapped in the DNA decreased the LOD to 0.01 nM [52] and finally, competitive interaction with intercalated DNA allowed record LOD of 0.6 pM [48]. The advantages of the DNA sensor proposed cover higher stability of the surface layer which retains its response to doxorubicin within three months of storage in dry conditions.…”

Section: Resultsmentioning

confidence: 99%

DNA-Polylactide Modified Biosensor for Electrochemical Determination of the DNA-Drugs and Aptamer-Aflatoxin M1 Interactions

Stepanova

Smolko

Gorbatchuk

et al. 2019

DNA sensors were assembled by consecutive deposition of thiacalix[4]arenes bearing oligolactic fragments, poly(ethylene imine), and DNA onto the glassy carbon electrode. The assembling of the layers was monitored with scanning electron microscopy, cyclic voltammetry and electrochemical impedance spectroscopy. The configuration of the thiacalix[4]arene core determined self-assembling of the polymeric species to the nano/micro particles with a size of 70–350 nm. Depending on the granulation, the coatings show the accumulation of a variety of DNA quantities, charges, and internal pore volumes. These parameters were used to optimize the DNA sensors based on these coatings. Thus, doxorubicin was determined to have limits of detection of 0.01 nM (cone configuration), 0.05 nM (partial cone configuration), and 0.10 nM (1,3-alternate configuration of the macrocycle core). Substitution of native DNA with aptamer specific to aflatoxin M1 resulted in the detection of the toxin in the range of 20 to 200 ng/L (limit of detection 5 ng/L). The aptasensor was tested in spiked milk samples and showed a recovery of 80 and 85% for 20 and 50 ng/L of the aflatoxin M1, respectively.