Voltammetric Sensor for Total Cholesterol Determination

Abstract

We report on a voltammetric sensor for the detection of total cholesterol. The sensor was fabricated by co-immobilization of two enzymes: cholesterol oxidase (ChOx) and horseradish peroxidase (HRP) on porous graphite. The electrochemical behavior of the sensor was studied with the use of linear sweep voltammetry. It has been shown that the sensor has high stability and high sensitivity (16 muA mM{-1} cm{-2}). The biosensor exhibited a wide linear range up to 300 mol/dm3 in a condition close to physiological (pH=6.86). Besides, the interferences of some key analytes containing in the blood were studied. As a matter of fact, making a fabricated sensor is rather promising for using in clinical practice

Square wave voltammetric detection of cholesterol with biosensor based on poly(styrene‐‐ε‐caprolactone)/MWCNTs composite

“…Besides, SWV and differential pulse voltammetry (DPV) are among the most utilized voltammetric techniques due to their high sensitivity 35 . In addition, voltammetric techniques as an analytical method, provide more accuracy than amperometric techniques 36 . However, there are few cholesterol biosensors conducted by the SWV technique 13,37,38 …”

“…35 In addition, voltammetric techniques as an analytical method, provide more accuracy than amperometric techniques. 36 However, there are few cholesterol biosensors conducted by the SWV technique. 13,37,38…”

Lipidest: a lipid profile screening test under extreme point of care settings using a portable spinning disc and an office scanner

“…The second-generation enzymatic biosensors use electron mediators to overcome this bottleneck. [44][45][46][47][48][49][50][51] Further improvements in the assay performance may be achieved by depositing a thin lm on nanostructured screen-printed electrodes. 52,53 However, the efficacies of these more sophisticated variants of the detection procedure are commonly based on the screen-printed electrode architecture, as well as the features of the counter electrode for a three-electrode system, 40 and the hematocrit correction electrode for a four-electrode system, 40 which are by no means trivial.…”

Review—Enzymatic Strips for Detection of Serum Total Cholesterol with Point-of-Care Testing (POCT) Devices: Current Status and Future Prospect

“…45 The main disadvantage of the measurement procedure of Abell-Kendall method is non-specificity to cholesterol, because some interfering compounds such as non-cholesterol sterols, cholesterol precursors, and oxidation products can produce chromophores which are measured at the same wavelength as cholesterol. 46 In conclusion, these methods, though relatively simple and inexpensive to perform, are not often used in POCT due to its complex multi-step procedures. Other non-enzymatic techniques such as gas-liquid chromatography, 47 high performance liquid chromatography (HPLC).…”

“…However, they depend on costly and large equipment and extensive sample pretreatment, which is also not suitable for the quick, convenient and economical detection in POCT. 46 On the contrast, since the invention of enzyme electrodes by Leland C. Clark, in 1962, 50 the enzymatic assays have been rapidly developed, evolving from costly, unstable laborious laboratory testing to the rapid-responsive, sensitive, stable and user-friendly analysis. 51 Furthermore, owning to inexpensive and miniaturized detection systems, colorimetric and electrochemical detection have become the optimal methods for POCT.…”