Voltammetric Sensor for Oxidized DNA Using Ultrathin Films of Osmium and Ruthenium Metallopolymers

Mugweru, Amos; Wang, Bingquan; Rusling, James F.

doi:10.1021/ac049375j

Cited by 64 publications

(41 citation statements)

References 57 publications

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“…29,30 Assembly of films on carbon surfaces still follows the same mechanism. 31 The mass increase M (g) for each adsorption step was estimated from the QCM frequency shifts DF (Hz) using the Saurbrey equation for the 5 MHz resonator characteristics.…”

Section: Film Characterization By Qcmmentioning

confidence: 99%

Redox Protein-Polymer Films for Simultaneous Determination of Ascorbic Acid and Hydrogen Peroxide

et al. 2008

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Section: Film Characterization By Qcmmentioning

confidence: 99%

Redox Protein-Polymer Films for Simultaneous Determination of Ascorbic Acid and Hydrogen Peroxide

et al. 2008

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“…The stability of PNA/DNA complexes in solution is therefore almost insensitive to the ionic strength ͑which is, in part, due to the noncharged PNA backbone͒. 3 Biomolecular interactions have been analyzed based on electrochemical data, 4 atomic force microscopy studies, 5 quartz crystal microbalance measurements, 6 and by other techniques. Among the optical methods, surface plasmon resonance ͑SPR͒ spectroscopy can be used to monitor in real time the interaction between molecules at the surface of a thin metal film, typically gold.…”

Section: Introductionmentioning

confidence: 99%

Kinetic and affinity analyses of hybridization reactions between peptide nucleic acid probes and DNA targets using surface plasmon field-enhanced fluorescence spectroscopy

et al. 2006

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Peptide nucleic acid (PNA), a polyamide DNA mimic, has inspired the development of a variety of hybridization-based methods for the detection, quantification, purification, and characterization of nucleic acids owing to the stability of the PNA/DNA duplex. In this work, PNA probes complementary to a specific sequence of Roundup Ready® soybean were immobilized onto a sensor surface via a self-assembled matrix employing streptavidin/biotin binding. The specific hybridization of PNA and DNA has been monitored by applying the chromophore-labeled DNA target oligonucleotides to the PNA modified Au sensor surface in real time using surface plasmon field-enhanced fluorescence spectroscopy. The authors demonstrate three kinds of experiments called global, titration, and kinetic analyses for the determination of rate constants for the association (kon) and dissociation processes (koff, and the affinity constant (KA) of the PNA/DNA duplex formation by fitting the data to a simple Langmuir model. Discrimination of a single base mismatched DNA (15mer) target on a 15mer PNA probe was documented, with a difference of the affinity constant of two orders of magnitude. Finally, the affinity constant for the hybridization of a long polymerase chain reaction product (169mer) obtained by amplification of DNA extracted from genetically modified soybean reference material has been determined by a kinetic-titration analysis. The results show the influence of a Coulomb barrier at high target surface coverage even for the hybridization to PNA at low ionic strength.

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“…The Os(II)/(III) redox potential is lowered when the osmium is complexed with ligands. We have previously used osmium redox polymers in construction of different biosensors [25,26]. The new material containing carbon nanofibers and osmium based redox polymers were used to fabricate a stable glucose sensor.…”

Section: Introductionmentioning

confidence: 99%

Carbon nanofiber modified with osmium based redox polymer for glucose sensing

Reaz

Ghosh

Wanekaya

2017

J. Electrochem. Sci. Eng.

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Electrochemical detection of glucose was performed on carbon nanofibers containing an osmium based redox polymer and using glucose oxidase enzyme. Redox polymer assembled on the nanofibers provided a more stable support that preserved enzyme activity and promoted the electrical communication to the glassy carbon electrode. The morphologies, structures, and electrochemical behavior of the redox polymer modified nanofibers were characterized by scanning electron microscope, energy dispersive spectrometer and voltammetry. The glucose oxidase showed excellent communication with redox polymer as observed with the increased activity toward glucose. Both cyclic voltammetry and amperometry showed a linear response with glucose concentration. The linear range for glucose determination was from 1 to 12 mM with a relatively high sensitivity of 0.20±0.01 μA mM −1 for glucose oxidase in carbon nanofibers and 0.10±0.01 μA mM −1 without carbon nanofibers. The apparent Michaelis-Menten constant (Km) for glucose oxidase with carbon nanofibers was 0.99 mM. On the other hand, the Km value for the glucose oxidase without the nanofibers was 4.90 mM.

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Voltammetric Sensor for Oxidized DNA Using Ultrathin Films of Osmium and Ruthenium Metallopolymers

Cited by 64 publications

References 57 publications

Redox Protein-Polymer Films for Simultaneous Determination of Ascorbic Acid and Hydrogen Peroxide

Redox Protein-Polymer Films for Simultaneous Determination of Ascorbic Acid and Hydrogen Peroxide

Kinetic and affinity analyses of hybridization reactions between peptide nucleic acid probes and DNA targets using surface plasmon field-enhanced fluorescence spectroscopy

Carbon nanofiber modified with osmium based redox polymer for glucose sensing

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