Voltammetric Sensing of Uremic Toxin Indoxyl Sulfate Using High Performance Disposable Screen-Printed Graphene Electrode

Filik, Hayati; Avan, Asiye Aslıhan; Aydar, Sevda

doi:10.2174/1573412911666150520000605

Cited by 7 publications

(9 citation statements)

References 4 publications

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“…It is likely that retesting it in a pure and larger population of spontaneous effusions would disclose better clinimetric properties. Secondly, we performed the voltammetric analysis according to the methods previously developed for the diagnosis of skin and bladder infections [5][6][7][8][9] . However, this might not be the best option for identifying a neoplastic pleural effusion.…”

Section: Discussionmentioning

confidence: 99%

“…This system has been used to measure different biological samples (urine, exudates collected from cutaneous ulcers) with promising results. VA can detect levels of uremic toxins 5 , urinary creatinine and the severity of renal insufficiency; it also allows to discriminate urine of healthy subjects from urine of patients with benign or malignant bladder 6 and prostate tumors 7 . Furthermore, it has been confirmed that VA is a powerful technique to diagnose UTI 8 .…”

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confidence: 99%

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Integration of voltammetric analysis, protein electrophoresis and pH measurement for diagnosis of pleural effusions: a non-conventional diagnostic approach

Pipita

Santonico

Pennazza

et al. 2020

Sci Rep

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Pleural effusion is very common, but an etiologic diagnosis is often difficult. We used three unconventional diagnostic techniques (voltammetric analysis, protein electrophoresis and pH measurement) performed on pleural effusion to do a preliminary distinction between a neoplastic and a non-neoplastic origin. Pleural fluid samples were collected through thoracentesis, thoracoscopy, or post-surgery pleural drainage of 116 patients admitted to acute care wards. Samples were analyzed with the three unconventional techniques: voltammetric analysis using the BIONOTE system, capillary electrophoresis and pH measurement using a potentiometric method. The BIONOTE system is an innovative system that performs a cyclic voltammetric analysis of a biological liquid sample. The final output of the electrochemical analysis is an electrical pattern that represents a fingerprint of the analyzed sample and each sample has a different fingerprint. Data from the three unconventional diagnostic techniques were analyzed using partial least squares discriminant analysis to discriminate neoplastic from non-neoplastic effusions; we also evaluated sensitivity, specificity and percentage of correct classification. The mean age was 68 years (SD: 12); 78 (67.24%) participants were men. Results obtained from all the unconventional techniques employed showed that neoplastic and non-neoplastic pleural effusions were correctly classified in 80.2% of cases, with a sensitivity of 77% and specificity of 83%. The combined use of voltammetric analysis, protein electrophoresis and pH measurement of pleural fluid can easily and quickly distinguish a neoplastic from a non-neoplastic pleural effusion with reliable accuracy and represents an innovative diagnostic approach. In fact, this protocol can be executed in just few minutes directly in the patient's bed and it holds great promise to improve the prognosis and therapeutic chances.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Integration of voltammetric analysis, protein electrophoresis and pH measurement for diagnosis of pleural effusions: a non-conventional diagnostic approach

Pipita

Santonico

Pennazza

et al. 2020

Sci Rep

View full text Add to dashboard Cite

show abstract

“…These methods have been applied for measuring polyamines, phenols, and indols in industrial applications. In a limited number, optical and electrochemical sensors have also been used for the analysis of UTs in biological samples, such as urine and blood; however, the measured values were not validated in method comparison studies using standard techniques, such as HPLC (38,39,87). Of note, a hospital's central laboratories measure many clinically important analytes with photometric methods in fully automated flow analytical systems.…”

Section: Instrumental Methods To Detect Utsmentioning

confidence: 99%

“…IDS was also measured in human serum and urine samples using cyclic and square wave voltammetry (Fig. 3) (38). Voltammetry was also used to detect indoxyl phosphate, following its hydrolysis by alkaline phosphatase to indigo blue (37).…”

Section: Detection Of Idsmentioning

confidence: 99%

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Generation, clearance, toxicity, and monitoring possibilities of unaccounted uremic toxins for improved dialysis prescriptions

Atherton

Bissler

Pendley

et al. 2018

American Journal of Physiology-Renal Physiology

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Current dialysis-dosing calculations provide an incomplete assessment of blood purification. They exclude clearances of protein-bound uremic toxins (PB-UTs), such as polyamines, p-cresol sulfate, and indoxyl sulfate, relying solely on the clearance of urea as a surrogate for all molecules accumulating in patients with end-stage renal disease (ESRD). PB-UTs clear differently in dialysis but also during normal renal function. The kidney clears PB toxins via the process of secretion, whereas it clears urea through filtration. Herein, we review the clearance, accumulation, and toxicity of various UTs. We also suggest possible methods for their monitoring toward the ultimate goal of a more comprehensive dialysis prescription. A more inclusive dialysis prescription would retain the kidney-filtration surrogate, urea, and consider at least one PB toxin as a surrogate for UTs cleared through cellular secretion. A more comprehensive assessment of UTs that includes both secretion and filtration is expected to result in a better understanding of ESRD toxicity and consequently, to reduce ESRD mortality.

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Analysis of indoxyl sulfate in biological fluids with emphasis on sample preparation techniques: A comprehensive analytical review

Shafiee,

Dastmalchi,

Gharekhani

et al. 2024

Heliyon

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Voltammetric Sensing of Uremic Toxin Indoxyl Sulfate Using High Performance Disposable Screen-Printed Graphene Electrode

Cited by 7 publications

References 4 publications

Integration of voltammetric analysis, protein electrophoresis and pH measurement for diagnosis of pleural effusions: a non-conventional diagnostic approach

Integration of voltammetric analysis, protein electrophoresis and pH measurement for diagnosis of pleural effusions: a non-conventional diagnostic approach

Generation, clearance, toxicity, and monitoring possibilities of unaccounted uremic toxins for improved dialysis prescriptions

Analysis of indoxyl sulfate in biological fluids with emphasis on sample preparation techniques: A comprehensive analytical review

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