Voltammetric measurements of neurotransmitter-acetylcholine through metallic nanoparticles embedded 2-D material

Tyagi, Chetna; Chauhan, Nidhi; Tripathi, Ambuj; Jain, Utkarsh; Avasthi, D.K.

doi:10.1016/j.ijbiomac.2019.08.102

Cited by 16 publications

(11 citation statements)

References 31 publications

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“…The biosensor showed an optimum response within 4 s, which is similar to biosensors based on AChE-ChO/GrNP/PtNPs/ITO-coated glass plates [ 16 ]. The optimum pH of the present biosensor was similar to that of earlier research based on AChE-ChO/AuNPs-GO/indium tin oxide (ITO)-coated glass plates [ 15 ]; closer to the optimum pH 7.4 of AChE-ChO/metallic organic framework (MOF) and AChE-ChO/PtNPs/AuE-based biosensors [ 14 ]; higher than biosensors based on AChE-ChO/Fe 2 O 3 NPs/rGO/PEDOT/fluorine-doped tin oxide (FTO) electrodes [ 17 ], AChE-ChO/MWCNT-manganese oxide MnO 2 /rGO/gold electrodes (AuEs) [ 13 ] and AChE-ChO/CDs-APTES/CPEs [ 19 ]; but lower than AChE-ChO/quantum dots (QDs)/reduced graphene oxide (rGO)/GCE biosensors [ 10 ] and biofunctional AMs-AChE-ChO/graphene–gold nanoparticles–chitosan (GR-AuNPs-CS)/GCE [ 9 ] and AChE-ChO/polypyrrole–polyvinyl sulfonate (PPy-PVS)/platinum (Pt) electrodes [ 12 ]. The optimum temperature of the present nanosensor (35 °C) is similar to biosensors based on AChE-ChO/GrNPs/PtNPs/ITO-coated glass plates [ 16 ].…”

Section: Resultssupporting

confidence: 82%

“…During this time, the original performance of the nanosensor decreased by 35% upon continuous use 250 times ( Figure 11 ). Therefore, the storage stability of the present biosensor was higher than earlier biosensors based on AChE-ChO/GrNP/PtNPs/ITO (4 months) [ 15 ] AChE-ChO/MWCNT-MnO 2 /rGO/Au (3 months) [ 13 ] and AChE-ChO/ePAMAM-Sal/CPE (1 month) [ 19 ]. Various analytical parameters of the present ACh biosensor were compared with those of earlier biosensors and are summarized in Table 2 .…”

Section: Resultsmentioning

confidence: 93%

“…Electrochemical biosensors based on the co-immobilization of enzymes onto different electrode surfaces have been reported [ 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 ]. These electrochemical biosensors convert biological signals into measurable signals [ 20 , 21 ].…”

Section: Introductionmentioning

confidence: 99%

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An Amperometric Acetylcholine Biosensor Based on Co-Immobilization of Enzyme Nanoparticles onto Nanocomposite

2023

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An electrochemical biosensor was fabricated using nanoparticles of acetylcholinesterase (AChE) and choline oxidase (ChO)/Pt nanoparticles (PtNPs)/porous graphene oxide nanosheet (GONS) composite. A pencil graphite electrode (PGE) was used for the electrodeposition of nanocomposite and the determination of acetylcholine (ACh), a neurotransmitter. Various techniques such as scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), Fourier-transform infrared (FTIR) spectra and cyclic voltammetry (CV) were used for characterization. This biosensor (AChENPs-ChONPs/GONS/PtNPs/PGE) indicated a very short response time (3 s), a lower limit of detection (0.001 µM), good linearity (0.001–200 µM), longer storage stability (6 months) and better reproducibility. The percent analytical recoveries of added acetylcholine in serum (5.0 and 10 µM) were found to be 97.6 ± 0.7 and 96.5 ± 0.3 for the present biosensor. The coefficients of variation were obtained to be 8% and 3.25%, correspondingly. The biosensor was applied to measure the ACh amount in the serum of healthy individuals and patients with Alzheimer’s disease. The number of interferents had no effect on the biosensor at their physiological concentrations.

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Section: Resultssupporting

confidence: 82%

Section: Resultsmentioning

confidence: 93%

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An Amperometric Acetylcholine Biosensor Based on Co-Immobilization of Enzyme Nanoparticles onto Nanocomposite

2023

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“…Yet, as the presentation of the biosensor significantly depends on the interfacial architecture of NMs, the enzyme must be immobilized in a way that promises the conservation of its organic activity and the availability of all available active sites along with the working efficiency of the transducing element. A functioning electrode made of an enzyme-based AuNPs-GO NC was produced on an ITO@ glass plate as shown in Figure 4 D [ 129 ]. The existence of AuNPs was confirmed by several characterization techniques, including UV-Visible spectroscopy, XRD, and SEM.…”

Section: Gr-based Ec Sensors For Bioanalytes Detectionmentioning

confidence: 99%

“…( D ) Depiction of Ach detection mechanism on the Surface of ITO-Au NPs-GO modified electrode. Reprinted with permission from [ 129 ].…”

Section: Figures Scheme and Tablesmentioning

confidence: 99%

The Roadmap of Graphene-Based Sensors: Electrochemical Methods for Bioanalytical Applications

et al. 2022

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Graphene (GR) has engrossed immense research attention as an emerging carbon material owing to its enthralling electrochemical (EC) and physical properties. Herein, we debate the role of GR-based nanomaterials (NMs) in refining EC sensing performance toward bioanalytes detection. Following the introduction, we briefly discuss the GR fabrication, properties, application as electrode materials, the principle of EC sensing system, and the importance of bioanalytes detection in early disease diagnosis. Along with the brief description of GR-derivatives, simulation, and doping, classification of GR-based EC sensors such as cancer biomarkers, neurotransmitters, DNA sensors, immunosensors, and various other bioanalytes detection is provided. The working mechanism of topical GR-based EC sensors, advantages, and real-time analysis of these along with details of analytical merit of figures for EC sensors are discussed. Last, we have concluded the review by providing some suggestions to overcome the existing downsides of GR-based sensors and future outlook. The advancement of electrochemistry, nanotechnology, and point-of-care (POC) devices could offer the next generation of precise, sensitive, and reliable EC sensors.

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Analyzing Electrochemical Sensing Fundamentals for Health Applications

Alam,

Mitea,

Howlader

et al. 2023

Advanced Sensor Research

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Humans continuously interact with physical, chemical, and biological environments that influence their health, safety, and quality of life. Sensing devices, such as electrochemical sensors that translate environmental qualities into electrical signals, are crucial for detecting biomarker concentrations in various biofluids. However, the understanding of electrochemical sensing is often incomplete, necessitating further study of chemical reactions and sensor‐electrode interactions for healthcare applications. This review analyzes crucial topics in chemical reactions in electrochemical sensing environments. First, the dynamics of chemical energy, the roles of acidic and alkaline fluids, chemical reaction tendencies, thermodynamic equilibria, Gibbs free energy, water dissociation, and the pH scale are discussed. Sensor materials or biomarkers undergo oxidation and reduction reactions in electrochemical sensing. Oxygen‐derived radicals and nonradical reactive species significantly influence biochemical reactions, cellular responses, and clinical outcomes. Then, the review delves into the impact of oxidation reduction reactions on human pathophysiology, redox reactions in hemoglobin, redox environments in human serum albumin and cells/tissues, and thermodynamics of biological redox reactions. Finally, recent advances in electrochemical techniques are presented and research challenges and future perspectives in electrochemical sensing for health applications are addressed.

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Voltammetric measurements of neurotransmitter-acetylcholine through metallic nanoparticles embedded 2-D material

Cited by 16 publications

References 31 publications

An Amperometric Acetylcholine Biosensor Based on Co-Immobilization of Enzyme Nanoparticles onto Nanocomposite

An Amperometric Acetylcholine Biosensor Based on Co-Immobilization of Enzyme Nanoparticles onto Nanocomposite

The Roadmap of Graphene-Based Sensors: Electrochemical Methods for Bioanalytical Applications

Analyzing Electrochemical Sensing Fundamentals for Health Applications

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