Voltammetric immunoassay for the human blood clotting factor IX by using nanogapped dielectrode junctions modified with gold nanoparticle-conjugated antibody

Gopinath, Subash C. B.; Perumal, Veeradasan; Balakrishnan, Sharma Rao; Arshad, M. K. Md; Voon, C. H.; Lakshmipriya, Thangavel; Haarindraprasad, R.; Vijayakumar, T. P.; Chen, Yeng; Hashim, U.

doi:10.1007/s00604-017-2389-7

Cited by 11 publications

(3 citation statements)

References 21 publications

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“…This research uses aptamer as the capturing probe and antibody as the detection probe to detect IGF1 at its lower level. Antibody is a well-known detection molecule efficiently used with various Immunosensing systems, such as Enzyme Linked Immunosorbent Assay, Western blot, Surface plasmon analysis, Waveguide-mode sensor and RAMAN spectroscopy [15][16][17][18]. Due to the higher stability in biological fluid, antibodies are used to detect the analytes in the body fluid, which includes saliva, serum, and sweat.…”

Section: Introductionmentioning

confidence: 99%

Biomolecular assembly on interdigitated electrode nanosensor for selective detection of insulin-like growth factor-1

Liu

Guo

et al. 2021

Artificial Cells, Nanomedicine, and Biotechnology

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This research is focussed to quantify IGF1 by electroanalytical analysis on InterDigitated electrode surface and characterized by the microscopic observations. For the detection, antibody and aptamer were used to analyze the level of IGF1. The sandwich pattern (aptamer-IGF1-antibody) was designed on the chemically modified IDE surface and reached the limit of detection to 10 fM with 100 folds enhancement in the sensitivity. Different control experiments (absence of IGF1, binding with IGF2 and with non-complementary aptamer) were failed to show the current changes, discriminated the specific detection. A good detection strategy is to complement the currently following imaging systems for AAA.

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Section: Introductionmentioning

confidence: 99%

Biomolecular assembly on interdigitated electrode nanosensor for selective detection of insulin-like growth factor-1

Liu

Guo

et al. 2021

Artificial Cells, Nanomedicine, and Biotechnology

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“…It was proved that antibody immobilization assisted by polyethylene glycol reduces the nonspecific absorption and improves the detection limit. Apart from that, nanoparticles are also utilized to immobilize the antibodies on the sensing surfaces at higher numbers [20][21][22]. At the same time, in most cases, multisteps procedure and cross-linking agent are needed to immobilize antibodies on the sensing surface.…”

Section: Introductionmentioning

confidence: 99%

Thyroglobulin determination on silane–antibody functionalized interdigitated dielectrode surface to diagnose thyroid tumor

Wang

Sun

Gopinath

et al. 2021

Biotech and App Biochem

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Thyroid cancer appears in endocrine glands and specific to thyroid glands has been reported widely. This work was targeted to identify and quantify thyroglobulin by using antithyroglobulin antibody complexed silane surface on interdigitated electrode (IDE) sensing surface.(3-Aminopropyl)triethoxysilane linker was used to make silane-coupling with antibody and attached on the hydroxylated IDE. This electroanalytical IDE revealed the dose-dependent responses with thyroglobulin concentrations. By getting increments with the thyroglobulin concentrations, the current responses were enhanced concomitantly and the thyroglobulin detection limit was noted as 1 pM on the linear curve [y = 0.1311x + 0.5386; R 2 = 0.9707] with the sensitivity at lower picomolar range. Moreover, the control experiments with thyroid peroxidase and nonimmune antibody cannot yield any response of current, confirming the specific detection of thyroglobulin. This research set-up is useful to determine and quantify the thyroglobulin and diagnose thyroid cancer.

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“…Gold as a transducer material has crucial characteristics, it can generate sensitive and selective electrochemical systems 19 . Gold has been broadly utilized in nanomedicine for the diagnosing purpose due to the outstanding merits, such as easy production method and fabrication process, chemically stable, and holds a great catalytic activity which can heading towards the formation of better biosensor platforms [20][21][22] .…”

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confidence: 99%

Comparative Analysis on Dielectric Gold and Aluminium Triangular Junctions: Impact of Ionic Strength and Background Electrolyte by pH Variations

Letchumanan

Arshad

Gopinath

et al. 2020

Sci Rep

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Field of generating a surface thin film is emerging broadly in sensing applications to obtain the quick and fast results by forming the high-performance sensors. Incorporation of thin film technologies in sensor development for the better sensing could be a promising way to attain the current requirements. This work predominantly delineates the fabrication of the dielectric sensor using two different sensing materials (Gold and Aluminium). conventional photolithography was carried out using silicon as a base material and the photo mask of the dielectric sensor was designed by AutocAD software. the physical characterization of the fabricated sensor was done by Scanning electron Microscope, Atomic Force Microscope, High Power Microscope and 3D-nano profiler. The electrical characterization was performed using Keithley 6487 picoammeter with a linear sweep voltage of 0 to 2 V at 0.01 V step voltage. By pH scouting, i-V measurements on the bare sensor were carried out, whereby the gold electrodes conducts a least current than aluminium dielectrodes. comparative analysis with pH scouting reveals that gold electrode is suitable under varied ionic strengths and background electrolytes, whereas aluminium electrodes were affected by the extreme acid (pH 1) and alkali (pH 12) solutions.

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Voltammetric immunoassay for the human blood clotting factor IX by using nanogapped dielectrode junctions modified with gold nanoparticle-conjugated antibody

Cited by 11 publications

References 21 publications

Biomolecular assembly on interdigitated electrode nanosensor for selective detection of insulin-like growth factor-1

Biomolecular assembly on interdigitated electrode nanosensor for selective detection of insulin-like growth factor-1

Thyroglobulin determination on silane–antibody functionalized interdigitated dielectrode surface to diagnose thyroid tumor

Comparative Analysis on Dielectric Gold and Aluminium Triangular Junctions: Impact of Ionic Strength and Background Electrolyte by pH Variations

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