Voltammetric Evaluation on Poly α-Aspartic Acid-Zinc Ion Complex in the Helix-coil Transition pH Region

Kimoto, Hiroki; Yanagisawa, Akira; Asano, Atsushi; Nakazawa, Chikako T.; Shinohara, Emi; Kurotsu, Takuzo

doi:10.2116/analsci.27.1157

Cited by 4 publications

(2 citation statements)

References 23 publications

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“…In contrast, poly( l -ornithine) and poly( l -aspartic acid) do not form α-helical structures even when charges are neutralized (Figure S8). , …”

Section: Resultsmentioning

confidence: 99%

“…33 In contrast, poly(L-ornithine) and poly(L-aspartic acid) do not form α-helical structures even when charges are neutralized (Figure S8). 34,35 Moreover, to investigate effects of pendant ureido or amino groups on the folding of PLOCs, 100 ns MD simulations of PLO and PLC were conducted. The root-mean-square deviations (RMSD) of PLO and PLC relative to initial αhelical structures were calculated to evaluate the stability of helical structures (Figure 5).…”

Section: Characterization Of Secondary Structures Of Pocsmentioning

confidence: 99%

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Highly Ordered Polypeptide with UCST Phase Separation Behavior

et al. 2018

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Manipulating phase separation structures of thermoresponsive polymers will enhance the usefulness of structure-controllable materials in fields such as drug delivery and tissue engineering. However, behaviors of upper critical solution temperature (UCST) have been less investigated so far, despite the importance of UCST. Here, we examined two citrulline-based polypeptides, poly(D-ornithine-co-D-citrulline) (PDOC) and poly(DL-ornithine-co-DL-citrulline) (PDLOC), to investigate how stereoregularity of the polypeptides influences UCST behavior, in addition to poly(L-ornithine-co-L-citrulline) (PLOC) previously studied. Homochiral PLOC and PDOC showed phase separation temperatures (T p s) higher than that of racemic PDLOC. Moreover, PDLOC underwent liquid to coacervate phase separation at T p , whereas PLOC and PDOC underwent liquid to solid-like aggregation transitions. From a structural point of view, circular dichroism and small-angle X-ray scattering measurements revealed that homochiral PLOC and PDOC polypeptides formed α-helical structures and assembled into a regular hexagonal lattice upon phase separation. Interactions between the pendent ureido groups of homochiral POCs appear to play pivotal roles in helical folding and assembly into the hexagonal structure. In addition, T p change in response to biodegradation was confirmed for both PLOC and PDLOC. The biodegradability was considerably influenced by phase-separated structures. These findings of UCST-type POCs in this study would provide important insights into structure-controllable and thermoresponsive biomaterials.

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“…In contrast, poly( l -ornithine) and poly( l -aspartic acid) do not form α-helical structures even when charges are neutralized (Figure S8). , …”

Section: Resultsmentioning

confidence: 99%