Voltammetric determination of the Escherichia coli DNA using a screen-printed carbon electrode modified with polyaniline and gold nanoparticles

Shoaie, Nahid; Forouzandeh, Mehdi; Omidfar, Kobra

doi:10.1007/s00604-018-2749-y

Cited by 77 publications

(30 citation statements)

References 31 publications

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“…Electrochemical DNA biosensors represent an exciting approach to the fast, low-cost detection of clinically important biomarkers [9,10] at the point of care [11]. A range of electrode materials and electrochemical measurement approaches have been employed for sensitive measurements (cyclic voltammetry [12], differential pulse voltammetry [13], square wave voltammetry [14] and electrochemical impedance spectroscopy [15]). The potential of electrochemical biosensors, once matured as a technology to provide efficient clinical workflows is immense.…”

Section: Introductionmentioning

confidence: 99%

Developing a Low-Cost, Simple-to-Use Electrochemical Sensor for the Detection of Circulating Tumour DNA in Human Fluids

et al. 2020

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It is well-known that two major issues, preventing improved outcomes from cancer are late diagnosis and the evolution of drug resistance during chemotherapy, therefore technologies that address these issues can have a transformative effect on healthcare workflows. In this work we present a simple, low-cost DNA biosensor that was developed specifically to detect mutations in a key oncogene (KRAS). The sensor employed was a screen-printed array of carbon electrodes, used to perform parallel measurements of DNA hybridisation. A DNA amplification reaction was developed with primers for mutant and wild type KRAS sequences which amplified target sequences from representative clinical samples to detectable levels in as few as twenty cycles. High levels of sensitivity were demonstrated alongside a clear exemplar of assay specificity by showing the mutant KRAS sequence was detectable against a significant background of wild type DNA following amplification and hybridisation on the sensor surface. The time to result was found to be 3.5 h with considerable potential for optimisation through assay integration. This quick and versatile biosensor has the potential to be deployed in a low-cost, point-of-care test where patients can be screened either for early diagnosis purposes or monitoring of response to therapy.

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Section: Introductionmentioning

confidence: 99%

Developing a Low-Cost, Simple-to-Use Electrochemical Sensor for the Detection of Circulating Tumour DNA in Human Fluids

et al. 2020

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“…Omidfar et al fabricated an ultrasensitive sensing platform for detecting E. coli utilizing surface modified screen‐printed carbon electrode by first polyaniline film and then AuNP. In the next step, avidin, followed by biotinylated DNA probe was immobilized covalently on the modified electrode by avidin–biotin interaction . The described electrochemical assay was found to detect 4*10 6 to 4 CFU of E. coli and concluded that this geneosensor had potential for accurate and rapid diagnosis of E. coli imposed infections.…”

Section: Intrinsically Conductive Polymer Composites For Biosensing Amentioning

confidence: 96%

Progress in the Development of Intrinsically Conducting Polymer Composites as Biosensors

Prajapati

Kandasubramanian

2019

Macro Chemistry & Physics

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Biosensors are analytical devices which find extensive applications in fields such as the food industry, defense sector, environmental monitoring, and in clinical diagnosis. Similarly, intrinsically conducting polymers (ICPs) and their composites have lured immense interest in bio‐sensing due to their various attributes like compatibility with biological molecules, efficient electron transfer upon biochemical reactions, loading of bio‐reagent, and immobilization of biomolecules. Further, they are proficient in sensing diverse biological species and compounds like glucose (detection limit ≈0.18 nm), DNA (≈10 pm), cholesterol (≈1 µm), aptamer (≈0.8 pm), and also cancer cells (≈5 pm mL−1) making them a potential candidate for biological sensing functions. ICPs and their composites have been extensively exploited by researchers in the field of biosensors owing to these peculiarities; however, no consolidated literature on the usage of conducting polymer composites for biosensing functions is available. This review extensively elucidates on ICP composites and doped conjugated polymers for biosensing functions of copious biological species. In addition, a brief overview is provided on various forms of biosensors, their sensing mechanisms, and various methods of immobilizing biological species along with the life cycle assessment of biosensors for various biosensing applications, and their cost analysis.

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“…Various graphene-based nanocomposites were also reported for the detection of Salmonella enterica and Escherichia coli [271,278,279]. Moreover, gold nanoparticles have also been extensively incorporated into electrochemical biosensors used in food born pathogen detection, due to their excellent physiochemical properties and biocompatibility [280][281][282][283].…”

Section: Electrochemical Biosensors For the Detection Of Foodborne Pamentioning

confidence: 99%

“…In voltammetric techniques such as cyclic voltammetry, differential pulse voltammetry, or square wave voltammetry, the current caused by an electrochemically active redox probe which is directly or indirectly related to the analyte concentration, is measured by varying the potential of the working/sensing electrode. Such voltammetric biosensor platforms have been successfully utilized to detect food-borne pathogens in recent years [282,284]. In amperometric biosensors, the potential of the sensing electrode is maintained at a constant value with respect to a reference electrode and the current is measured as a function time [285].…”

Section: Electrochemical Biosensors For the Detection Of Foodborne Pamentioning

confidence: 99%

QCM Sensor Arrays, Electroanalytical Techniques and NIR Spectroscopy Coupled to Multivariate Analysis for Quality Assessment of Food Products, Raw Materials, Ingredients and Foodborne Pathogen Detection: Challenges and Breakthroughs

Bwambok

Siraj

Macchi

et al. 2020

Sensors

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Quality checks, assessments, and the assurance of food products, raw materials, and food ingredients is critically important to ensure the safeguard of foods of high quality for safety and public health. Nevertheless, quality checks, assessments, and the assurance of food products along distribution and supply chains is impacted by various challenges. For instance, the development of portable, sensitive, low-cost, and robust instrumentation that is capable of real-time, accurate, and sensitive analysis, quality checks, assessments, and the assurance of food products in the field and/or in the production line in a food manufacturing industry is a major technological and analytical challenge. Other significant challenges include analytical method development, method validation strategies, and the non-availability of reference materials and/or standards for emerging food contaminants. The simplicity, portability, non-invasive, non-destructive properties, and low-cost of NIR spectrometers, make them appealing and desirable instruments of choice for rapid quality checks, assessments and assurances of food products, raw materials, and ingredients. This review article surveys literature and examines current challenges and breakthroughs in quality checks and the assessment of a variety of food products, raw materials, and ingredients. Specifically, recent technological innovations and notable advances in quartz crystal microbalances (QCM), electroanalytical techniques, and near infrared (NIR) spectroscopic instrument development in the quality assessment of selected food products, and the analysis of food raw materials and ingredients for foodborne pathogen detection between January 2019 and July 2020 are highlighted. In addition, chemometric approaches and multivariate analyses of spectral data for NIR instrumental calibration and sample analyses for quality assessments and assurances of selected food products and electrochemical methods for foodborne pathogen detection are discussed. Moreover, this review provides insight into the future trajectory of innovative technological developments in QCM, electroanalytical techniques, NIR spectroscopy, and multivariate analyses relating to general applications for the quality assessment of food products.

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Voltammetric determination of the Escherichia coli DNA using a screen-printed carbon electrode modified with polyaniline and gold nanoparticles

Cited by 77 publications

References 31 publications

Developing a Low-Cost, Simple-to-Use Electrochemical Sensor for the Detection of Circulating Tumour DNA in Human Fluids

Developing a Low-Cost, Simple-to-Use Electrochemical Sensor for the Detection of Circulating Tumour DNA in Human Fluids

Progress in the Development of Intrinsically Conducting Polymer Composites as Biosensors

QCM Sensor Arrays, Electroanalytical Techniques and NIR Spectroscopy Coupled to Multivariate Analysis for Quality Assessment of Food Products, Raw Materials, Ingredients and Foodborne Pathogen Detection: Challenges and Breakthroughs

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