Voltammetric determination of taurine in energy drinks after o-phthalaldehyde-ethanethiol derivatization

Farag, Amir Shaaban; Klikarová, Jitka; Česlová, Lenka; Vytřas, Karel; Sýs, Milan

doi:10.1016/j.talanta.2019.04.083

Cited by 12 publications

(4 citation statements)

References 46 publications

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“…Taurine (2-aminoethanesulfonic acid), the main endproduct of cysteine metabolism in eukaryotes, can be synthesized through metabolic engineering and chemical synthesis [22]. As a semi-essential micro-nutrient, taurine was always biologically and physiologically used in food, energy drinks and medicine [22][23][24]. Although used as a sulfur source for fermenting bacteria [25], the effect of taurine on product accumulation of M. purpureus is unknown.…”

Section: Introductionmentioning

confidence: 99%

Taurine-mediated gene transcription and cell membrane permeability reinforced co-production of bioethanol and Monascus azaphilone pigments for a newly isolated Monascus purpureus

Yi,

Han,

et al. 2024

Biotechnol Biofuels

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Background Taurine, a semi-essential micronutrient, could be utilized as a sulfur source for some bacteria; however, little is known about its effect on the accumulation of fermentation products. Here, it investigated the effect of taurine on co-production of bioethanol and Monascus azaphilone pigments (MonAzPs) for a fungus. Results A newly isolated fungus of 98.92% identity with Monascus purpureus co-produced 23.43 g/L bioethanol and 66.12, 78.01 and 62.37 U/mL red, yellow and orange MonAzPs for 3 d in synthetic medium (SM). Taurine enhanced bioethanol titer, ethanol productivity and ethanol yield at the maximum by 1.56, 1.58 and 1.60 times than those of the control in corn stover hydrolysates (CSH), and red, yellow and orange MonAzPs were raised by 1.24, 1.26 and 1.29 times, respectively. Taurine was consumed extremely small quantities for M. purpureus and its promotional effect was not universal for the other two biorefinery fermenting strains. Taurine intensified the gene transcription of glycolysis (glucokinase, phosphoglycerate mutase, enolase and alcohol dehydrogenase) and MonAzPs biosynthesis (serine hydrolases, C-11-ketoreductase, FAD-dependent monooxygenase, 4-O-acyltransferase, deacetylase, NAD(P)H-dependent oxidoredutase, FAD-dependent oxidoredutase, enoyl reductase and fatty acid synthase) through de novo RNA-Seq assays. Furthermore, taurine improved cell membrane permeability through changing cell membrane structure by microscopic imaging assays. Conclusions Taurine reinforced co-production of bioethanol and MonAzPs by increasing gene transcription level and cell membrane permeability for M. purpureus. This work would offer an innovative, efficient and taurine-based co-production system for mass accumulation of the value-added biofuels and biochemicals from lignocellulosic biomass.

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Section: Introductionmentioning

confidence: 99%

Taurine-mediated gene transcription and cell membrane permeability reinforced co-production of bioethanol and Monascus azaphilone pigments for a newly isolated Monascus purpureus

Yi,

Han,

et al. 2024

Biotechnol Biofuels

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“…Energy drinks have recently become popular beverages among young adults, 1 who consume energy drinks to maintain physical comfort, improve performance and awareness, and stimulate thought processes. 2 In addition to other active ingredients, taurine is a major constituent of energy drinks as well as infant formulations and dietary supplements.…”

Section: Introductionmentioning

confidence: 99%

Utilization of 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F) as a fluorogenic reagent for the development of a spectrofluorometric assay method for taurine in energy drinks

Alnajjar

Elbashir

Elgorashe

et al. 2022

Journal of Chemical Research

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The development of a simple, inexpensive, sensitive, and selective spectrofluorometric assay method for taurine in energy drinks utilizing 4-fluoro-7-nitro-2,1,3-benzoxadiazole as a fluorogenic agent is described. Conditions affecting the derivatization reaction are investigated and optimized. The optimal conditions are found to be as follows: buffer, pH 10; 4-fluoro-7-nitro-2,1,3-benzoxadiazoleconcentration, 0.05%; reaction time, 30 min; temperature, 50 °C. Fluorescence measurements are carried out at a wavelength of 542 nm, with excitation at a wavelength of 485 nm. The method is validated under the optimum conditions. The method is found to be linear in the range of 2.0–12.5 µg/mL, with a correlation coefficient of 0.9993. The limits of detection and quantification (µg/mL) are 0.6 and 2.02, respectively, which are appropriate for taurine assays in commercial energy drinks. In addition, the proposed method recorded excellent accuracy since the recovery values caused by presence of excipients are found to be in the range of 99.3%–102.87%. The values of relative standard deviation for intra- and inter-day precision were found to be in the ranges of 0.236%–0.659% and 2.12%–2.63%, respectively.

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“…Taurine as substrate for B. wadsworthia can enter the human digestive system as part of the diet, e.g. as a constituent of meat [ 14 , 15 ] and energy drinks [ 16 ], while isethionate can be found in seafood [ 17 , 18 ]. More importantly, taurine is produced by the human body, for example as a conjugate for bile salts such as taurocholate [ 19 – 21 ].…”

Section: Introductionmentioning

confidence: 99%

Bacterial microcompartments for isethionate desulfonation in the taurine-degrading human-gut bacterium Bilophila wadsworthia

et al. 2021

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Background Bilophila wadsworthia, a strictly anaerobic, sulfite-reducing bacterium and common member of the human gut microbiota, has been associated with diseases such as appendicitis and colitis. It is specialized on organosulfonate respiration for energy conservation, i.e., utilization of dietary and host-derived organosulfonates, such as taurine (2-aminoethansulfonate), as sulfite donors for sulfite respiration, producing hydrogen sulfide (H2S), an important intestinal metabolite that may have beneficial as well as detrimental effects on the colonic environment. Its taurine desulfonation pathway involves the glycyl radical enzyme (GRE) isethionate sulfite-lyase (IslAB), which cleaves isethionate (2-hydroxyethanesulfonate) into acetaldehyde and sulfite. Results We demonstrate that taurine metabolism in B. wadsworthia 3.1.6 involves bacterial microcompartments (BMCs). First, we confirmed taurine-inducible production of BMCs by proteomic, transcriptomic and ultra-thin sectioning and electron-microscopical analyses. Then, we isolated BMCs from taurine-grown cells by density-gradient ultracentrifugation and analyzed their composition by proteomics as well as by enzyme assays, which suggested that the GRE IslAB and acetaldehyde dehydrogenase are located inside of the BMCs. Finally, we are discussing the recycling of cofactors in the IslAB-BMCs and a potential shuttling of electrons across the BMC shell by a potential iron-sulfur (FeS) cluster-containing shell protein identified by sequence analysis. Conclusions We characterized a novel subclass of BMCs and broadened the spectrum of reactions known to take place enclosed in BMCs, which is of biotechnological interest. We also provided more details on the energy metabolism of the opportunistic pathobiont B. wadsworthia and on microbial H2S production in the human gut.

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Voltammetric determination of taurine in energy drinks after o-phthalaldehyde-ethanethiol derivatization

Cited by 12 publications

References 46 publications

Taurine-mediated gene transcription and cell membrane permeability reinforced co-production of bioethanol and Monascus azaphilone pigments for a newly isolated Monascus purpureus

Taurine-mediated gene transcription and cell membrane permeability reinforced co-production of bioethanol and Monascus azaphilone pigments for a newly isolated Monascus purpureus

Utilization of 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F) as a fluorogenic reagent for the development of a spectrofluorometric assay method for taurine in energy drinks

Bacterial microcompartments for isethionate desulfonation in the taurine-degrading human-gut bacterium Bilophila wadsworthia

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