Voltammetric determination of sequence-specific DNA by electroactive intercalator on graphite electrode

Liu, Shenghui; Ye, Jiannong; He, Pingang; Fang, Yuzhi

doi:10.1016/s0003-2670(96)00331-5

Cited by 56 publications

(39 citation statements)

References 10 publications

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“…56,58,59 DNA-based electrochemical methods have also been developed primarily to detect nucleic acid hybridization. 44,45,[60][61][62][63][64][65][66][67][68][69][70][71][72][73] Notably, those methods do not rely on an intercalator as the redox probe. General principles governing electron transport through modified films involving percolation or the physical displacement of associated ions are also well understood.…”

Section: Introductionmentioning

confidence: 99%

Intercalative Stacking: A Critical Feature of DNA Charge-Transport Electrochemistry

et al. 2003

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In electrochemistry experiments on DNA-modified electrodes, features of the redox probe that determine efficient charge transport through DNA-modified surfaces have been explored using methylene blue (MB + ) and Ru(NH 3 ) 6 3+ as DNA-binding redox probes. The electrochemistry of these molecules is studied as a function of ionic strength to determine the necessity of tight binding to DNA and the number of electrons involved in the redox reaction; on the DNA surface, MB + displays 2e -/1H + electrochemistry (pH 7) and Ru(NH 3 ) 6 3+ displays 1e -electrochemistry. We examine also the effect of electrode surface passivation and the effect of the mode (intercalation or electrostatic) of MB + and Ru(NH 3 ) 6 3+ binding to DNA to highlight the importance of intercalation for reduction by a DNA-mediated charge-transport pathway. Furthermore, in experiments in which MB + is covalently linked to the DNA through a σ-bonded tether and the ionic strength is varied, it is demonstrated that intercalative stacking rather than covalent σ-bonding is essential for efficient reduction of MB + . The results presented here therefore establish that efficient charge transport to the DNA-binding moiety in DNA films requires intercalative stacking and is mediated by the DNA base pair array.

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Section: Introductionmentioning

confidence: 99%

Intercalative Stacking: A Critical Feature of DNA Charge-Transport Electrochemistry

et al. 2003

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“…For the development of electrochemical methods to analyze the DNA sequences, the following strategies are successfully applying as reporters redox molecules capable to noncovalently bind to DNA duplexes, e.g., minor groove binding ligands such as Hoechst 33258 [68] or intercalators, e.g., methylene blue [49], doxorubicin [69], ethidium bromide, [70], as well as antibiotics and other dyes. Using this approach, the detection limit of the analyzed DNA sequence is ~ 10 -11 M [48].…”

Section: Electrochemical Biosensorsmentioning

confidence: 99%

Existing Biosensing Platforms for the Nucleotide Markers Detection

Dmitrienko

Pyshnaya

Dultsev

et al. 2016

Preprint

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Abstract:In review, the operating principles of the most common bio sensing devices, detection methods and the identification sensitivity of analyzed molecules were shown. The central focus was done on hybridization analysis of nucleic acids (NA), which are considered to be one of the most important analytes in terms of diagnostic point of view. Constructions enabling to transfer the fact of formation of nucleotide probe/target complex in to detectable signal by optical, electrochemical or micromechanical (piezoelectric) sensors were presented in this review.

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“…PSA and cyclic voltammetry can quantify DNA or RNA at 1.0 -2.5 pg/µl utilizing carbon paste or graphite electrodes (66)(67)(68)(69), detection sensitivities almost on par with fluorescent methods (in an environmental context). Highly sensitive electrochemical measurements are possible because DNA is accumulated at the electrode prior to the electrochemical measurement, a mass action principle not possible with passive adsorption methods.…”

Section: Electrochemistrymentioning

confidence: 99%

Advances Towards Integrated Biodetection Systems for Environmental Molecular Microbiology

Chandler

2002

Current Issues in Molecular Biology

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To overcome many of the limitations associated with indirect detection methods, new techniques for the sensitive, specific, and direct detection of nucleic acids are required in order to accurately and quantitatively ascribe phenotype/function to uncultivated microorganisms. However, if advanced diagnostic and detection systems are going to be applied in environmental microbiology, future "biodetection" technologies and systems must be developed not from the point of view of the detector, but from the unique aspects of the environmental sample and the entire analytical process. This article highlights recent advances in nucleic acid-based technologies, and looks towards future advances that may address the broad needs and conditions imposed by environmental molecular microbiology.

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Voltammetric determination of sequence-specific DNA by electroactive intercalator on graphite electrode

Cited by 56 publications

References 10 publications

Intercalative Stacking: A Critical Feature of DNA Charge-Transport Electrochemistry

Intercalative Stacking: A Critical Feature of DNA Charge-Transport Electrochemistry

Existing Biosensing Platforms for the Nucleotide Markers Detection

Advances Towards Integrated Biodetection Systems for Environmental Molecular Microbiology

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