Voltammetric determination of parthenolide in spiked human plasma and urine

Abstract

The voltammetric behaviour of parthenolide, a biologically active sesquiterpene lactone, was studied using direct current (DCt), alternating current and differential-pulse polarography (DPP). Parthenolide developed well-defined cathodic waves over the whole pH range in Britton-Robinson buffers. At pH 10 the diffusion current constant was 3.54 +/- 0.08 (+/- standard deviation; n = 8). The current vs concentration plots were rectilinear over the range 4-36 and 1-28 micrograms/mL in the DCt and DPP modes, respectively, with a minimum detectability of 0.06 microgram/mL (about 1 x 10(-7) M) using the latter technique. The waves were characterised as being diffusion controlled, although adsorption phenomenon played a limited role in the electrode process. The described analytical method was applied to the determination of parthenolide in spiked human urine and plasma; the percentage recoveries were 95.72 +/- 0.22 and 94.0 +/- 0.13 (+/- standard deviation; n = 9), respectively.

Natural Products and their Role as Inhibitors of the Pro-Inflammatory Transcription Factor NF-κB

“…Such bioavailability could be possible if the protein-bound parthenolide maintained a therapeutic action. The presence of parthenolide has also been successfully quantified in spiked samples of human plasma (Belal et al, 2001) …”

Natural sesquiterpenoids

“…252 The voltammetric determination of parthenolide in spiked human plasma and urine has been investigated. 253 Comparative studies of the antioxidant properties and the growth regulation activity of ovatifolin and other sesquiterpene lactones have been carried out. 254 The full paper describing the ultrasound assisted reductive cleavage of several sesquiterpene γ-enonelactones to form the corresponding acids has appeared.…”