Voltammetric Determination of Aflatoxin B1

Gavrilova, M. A.; Слепченко, Г. Б.; Mikheeva, Elena Valentionovna; Derybina, V.I.

doi:10.1016/j.proche.2014.10.021

Cited by 7 publications

(5 citation statements)

References 13 publications

(8 reference statements)

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“…Studies on the effect of the background electrolyte composition on the analytical signals of aflatoxins В 1 and М 1 on a glassy-carbon electrode under working conditions previously developed for the determination of aflatoxin В 1 were conducted [ 24 ]. Experiments on the choice of the background electrolyte showed that the value of the analytical signal aflatoxin M 1 on background electrolytes: 0.1 M Na 3 PO 4 , 0.1 M Na 2 HPO 4 , 0.1 M K 2 HPO 4 , and 0.1 M ZnSO 4 , was found to be low, and it was high on background electrolytes: 0.1 M (NH 4 ) 2 SO 4 and 0.1 M Li 2 CO 3 ; the maximum current of its electric oxidation was obtained against the background of 0.1 M C 6 H 5 O 7 (NH 4 ) 3 .…”

Section: Resultsmentioning

confidence: 99%

The Simultaneous Voltammetric Determination of Aflatoxins В₁ and М₁ on a Glassy-Carbon Electrode

Слепченко

Gindullina

Gavrilova

et al. 2018

Journal of Analytical Methods in Chemistry

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For the first time, the possibility of using stripping voltammetry for the simultaneous determination of aflatoxins В1 and М1 on a glassy-carbon electrode has been shown. The influence of various factors (Еэ, τэ, w, and the nature of the background electrolyte) on the potential and magnitude of the oxidation current of mycotoxins has been estimated. Working conditions for voltammetric determination and reproducibility of analytical signals for two mycotoxins have been selected. The mutual influence of aflatoxins В1 and М1 on the value of analytical signals in their simultaneous presence has been studied. It has been found that, in the range of their detectable contents, the presence of aflatoxin В1 reduces the analytical signal of aflatoxin М1 by 45–50%, but the linearity of the calibration dependence is preserved. The content of aflatoxin М1 in determination of aflatoxin B1 does not exert a significant effect in the range of 10–15%. Based on the results obtained, a procedure has been proposed for determining the content of aflatoxins В1 and М1 in their joint presence in milk by voltammetry in the concentration ranges 2 × 10−3 ÷ 2 × 10−1 mg/dm3 and 2 × 10−4 ÷ 2 × 10−2 mg/dm3, respectively (Sr not more than 18%).

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Section: Resultsmentioning

confidence: 99%

The Simultaneous Voltammetric Determination of Aflatoxins В₁ and М₁ on a Glassy-Carbon Electrode

Слепченко

Gindullina

Gavrilova

et al. 2018

Journal of Analytical Methods in Chemistry

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“…Aflatoxins are absorbed in the gastrointestinal tract but not been biotransformed in the liver can also be excreted (Scaglioni et al, 2014). Thus aflatoxin can accumulate through the food chain posing a serious health concern to both humans and animals (Otim et al, 2005;Gavrilova et al, 2014;Patel et al, 2015). However, according to the survey conducted throughout North western Italy between 2012 and 2014, the overall AFM contamination rate was 2.2% (36 samples out of 1668 samples) and less than 1% of milk samples were non-compliant with EU limits (Bellio et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

“…Aflatoxins are potent toxic, carcinogenic, mutagenic and immunosuppressive agents produced as secondary metabolites by Aspergillus flavus and Aspergillus parsisticus on a variety of food products (Ozay et al, 2008). The levels of aflatoxins contamination is a cumulative and therefore are associated with acute and chronic toxicities to both humans and animals (Otim et al, 2005;Gavrilova et al, 2014;Patel et al, 2015;El Zubeir, 2023). The higher occurrence of aflatoxin M1 in raw and processed milk samples might have a negative impact on public health, especially infants and children who consumed large amount of fluid milk (Fadlalla et al, 2020;Yousof and El Zubeir, 2020).…”

Section: Introductionmentioning

confidence: 99%

Chemical Composition and Screening of Aflatoxin in Cows’ Milk in Gadarif town, Sudan

Abdalmahmoud,

Shuiep,

El Zubeir

et al. 2023

VMPH

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Objectives: This study was conducted to analyze milk samples, for chemical composition and detection of aflatoxins M1 in Gedarif town, East Sudan. Methods: One hundred milk samples were collected randomly; 35 samples from farms, 35 samples from sale points and 30 samples from groceries in Gedarif town. Milk-Kana was used for determination of fat, solids not fat, lactose and protein, freezing point, density and added water and Unisensor kits were used as screening method for detection of aflatoxin M1. Results: The collected milk samples showed lower content of fat (3.4 +- 1.3%, 3.4 +- 1.3% and 3.5 +- 1.25%), solids not fat (7.7 +-1.1%, 8.1+-1.7% and 8+-1%), lactose (4.2+-0.6%, 4.4+-0.8% and 4.3+-0.5%) and protein (3+-0.4%, 3+-0.5% and 3+-0.4%). Relatively, the freezing point and density of milk revealed few variations in the examined samples. Moreover, the added water was high in the samples collected from the farms and sale points compared to those from the groceries. The occurrence of aflatoxin M1 was found in 22 (27.50%) of the milk samples. The presence of aflatoxin contamination was high in milk samples collected from sales points (15.0%) followed by farms (11.25%) compared to those obtained from groceries (1.25%). Conclusions: The presences of aflatoxin in the milk samples might indicate that the cow milk was contaminated with aflatoxins through feed. Hence this study recommended that good hygienic practices should be considered in Gadarif town. Similarly, regulations and lows for selling the milk should be implemented by the official authorities.

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“…Nowadays, mycotoxin contamination resistor difficulties are mainly applicable and resolved not only within specific states, but also worldwide, under the support of the Food and Agriculture Organization and World Health Organization [1]. Aflatoxins are secondary toxic metabolites produced by numerous fungi such as Aspergillus flavus, Aspergillus parasiticus, Aspergillus nomius, and Aspergillus pseudotamarii [2]. Aflatoxin B1 (AFA−B1) is also known as 2,3,6a R ,9a S ‐tetrahydro‐4‐methoxy‐1 H ,11 H ‐cyclopenta[c]furo[3′,2′:4,5]furo[2,3 h]benzopyran‐1,11‐dione (Figure 1).…”

Section: Introductionmentioning

confidence: 99%

Fe₃O₄ Nanorods‐RGO‐ionic Liquid Nanocomposite Based Electrochemical Sensor for Aflatoxin B1 in Ground Paprika

Chokkareddy

Redhi

2021

Electroanalysis

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A novel and sensitive method for the determination of aflatoxin B1 (AFA−B1) in ground paprika using a methyltrioctylammonium chloride ionic liquid (IL), iron oxide nanorods (Fe3O4 nanorods) and reduced graphene oxide (RGO) fabricated glassy carbon electrode (GCE) was developed. The synthesized nanoparticles, nanocomposites and modified electrode surfaces were characterized by Fourier transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM), thermogravimetric analysis (TGA/DSC) and x‐ray diffraction (XRD) analyses. Moreover, the electrochemical performance of the developed sensor was determined by cyclic voltammetry (CV) and differential pulse voltammetry (DPV). The obtained results demonstrate that the sensitivity of AFA−B1 is significantly enhanced on RGO‐Fe3O4 nanorods‐IL‐GCE in comparison with bare GCE, RGO‐GCE and RGO‐Fe3O4 nanorods‐GCE. The redox peak currents of AFA−B1 exhibited good linear relationship with its concentration in the range from 0.02 to 0.33 ng mL−1 with detection limit of (LOD) 0.03 ng mL−1 and limit of quantification (LOQ) 0.36 ng mL−1 respectively (S/N=3). In addition, the fabricated electrode showed good stability and reproducibility. The proposed technique was effectively applied to identify the AFA−B1 in real ground paprika samples with acceptable results.

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Voltammetric Determination of Aflatoxin B1

Cited by 7 publications

References 13 publications

The Simultaneous Voltammetric Determination of Aflatoxins В₁ and М₁ on a Glassy-Carbon Electrode

The Simultaneous Voltammetric Determination of Aflatoxins В₁ and М₁ on a Glassy-Carbon Electrode

Chemical Composition and Screening of Aflatoxin in Cows’ Milk in Gadarif town, Sudan

Fe₃O₄ Nanorods‐RGO‐ionic Liquid Nanocomposite Based Electrochemical Sensor for Aflatoxin B1 in Ground Paprika

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Voltammetric Determination of Aflatoxin B1

Cited by 7 publications

References 13 publications

The Simultaneous Voltammetric Determination of Aflatoxins В1 and М1 on a Glassy-Carbon Electrode

The Simultaneous Voltammetric Determination of Aflatoxins В1 and М1 on a Glassy-Carbon Electrode

Chemical Composition and Screening of Aflatoxin in Cows’ Milk in Gadarif town, Sudan

Fe3O4 Nanorods‐RGO‐ionic Liquid Nanocomposite Based Electrochemical Sensor for Aflatoxin B1 in Ground Paprika

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The Simultaneous Voltammetric Determination of Aflatoxins В₁ and М₁ on a Glassy-Carbon Electrode

The Simultaneous Voltammetric Determination of Aflatoxins В₁ and М₁ on a Glassy-Carbon Electrode

Fe₃O₄ Nanorods‐RGO‐ionic Liquid Nanocomposite Based Electrochemical Sensor for Aflatoxin B1 in Ground Paprika