Voltammetric Detection of the DNA Interaction with Copper Complex Compounds and Damage to DNA

Abstract

Binding reactions of copper(II) complexes of 1,10-phenanthroline (phen) and tetraaza macrocyclic ligand TAAB with calf thymus DNA have been investigated voltammetrically at bare and DNA modi®ed glassy carbon electrodes. The values of binding constant, binding site size, ratio of binding constants for the reduced and oxidized copper complex forms as well as information on the electrostatic and intercalative binding modes were obtained by solution and surface-based methods. The Cu(phen) 2 2 complex mediates the dsDNA cleavage to a higher degree than Cu(TAAB) 2 as indicated by the redox marker Co(phen) 3 3and the anodic signal of the DNA base. A procedure for the damage to DNA detection using dsDNAaGCE biosensor is proposed.

Detection of Antioxidative Activity of Plant Extracts at the DNA-Modified Screen-Printed Electrode

“…Voltammetric behavior of [Co(phen) 3 ] 3+ at the DNA-modified gold electrode [24], glassy carbon electrode [20], carbon paste electrode [25,26] as well as carbon-based screen-printed electrode [18,19] was reported previously. After the accumulation of the marker at DNA/SPE under the open circuit conditions, a good developed DPV peak of the complex appears at -0.130 V vs Ag/AgCl (Fig.…”

“…This scheme is based on the known quantification of the original double stranded (ds) DNA using the [Co(phen) 3 ] 3+ complex ion as a dsDNA electrochemical label. The amount of [Co(phen) 3 ] 3+ bound to the DNA layer decreases with a decrease in the portion of original dsDNA in the course of cleavage reaction [20,21]. Two standards of phenolic acids as well as four aqueous plant extracts were tested as the antioxidants.…”

Voltammetric Detection of Antioxidative Properties of Flavonoids Using Electrically Heated DNA Modified Carbon Paste Electrode

“…An effective preconcentration of the indicator within DNA can be seen from the peak current values. A negative E p shift at the DNA/CPE comparing to the CPE confirms predominantly electrostatic interaction with DNA in 5 mmol/l buffer solution [9,26]. Hence, [Co(phen) 3 ]…”

“…The strategy is based on the employment of DNA itself as the sensor biocomponent for the detection of species leading to damage or protection of DNA. Mercury as well as solid electrodes were used as electrochemical transducers of a signal [8][9][10][11]. Electrically heated electrodes represent a way of enhancement of the sensitivity of electrochemical analysis [12,13].…”

The Interaction of DNA with Dopamine by Spectroscopic and Electrochemical Methods

“…However, DNA molecules are prone to be damaged under various conditions, such as radiation or interactions with some other molecules; this damage would probably lead to various pathological changes in living organisms. Studies on the binding mechanism of some small molecules with DNA have consequently been a hot topic during the past few tens of years, [1][2][3][4][5] since it is of great help to understand the structural properties of DNA, the mutation of genes, the origins of some diseases, the action mechanisms of some antitumor and antivirus drugs and, therefore, to design new and more efficient DNA-targeted drugs to deal with genetic diseases. Additional, it also helps us to seek new probes for nucleic acid analysis.…”