Voltammetric Behavior of Chlorophyllaat a Screen‐Printed Carbon Electrode and Its Potential Role as a Biomarker for Monitoring Fecal Contamination

Abstract

Direct cyclic voltammetric determination of Chlorophyll a (Chl a) at a screen-printed carbon electrode (SPCE) resulted in a single, irreversible anodic oxidation peak at E p ¼ þ400 mV vs. Ag/AgCl. Electrochemical investigations revealed that Chl a was adsorbing onto the SPCE surface. This adsorption phenomenon allowed the development of a method for Chl a determination, based on medium exchange followed by adsorptive stripping voltammetry (AdSV). The final protocol was optimised with ORDER REPRINTS respect to pH of accumulation/measurement buffer, accumulation time, and acetone content of accumulation buffer. Using this protocol, it was possible to determine Chl a in aqueous phosphate buffer over the concentration range 0.014-2.24 mM. This optimised protocol was applied to the determination of Chl a in faeces from dairy cows. The endogenous content of Chl a was calculated to be 15 mg g 21 of dried faecal matter. The mean recovery for the method was 104%, with an overall coefficient of variation of 18.4%. Based on these data, the detection limit for dried faecal matter was 300 mg in 10 mL of simulated teat wash water. This approach demonstrates that the electrochemical detection of Chl a is a potential means of monitoring faecal contamination in the dairy industry.

Electrochemical Evaluation of Sweet Sorghum Fermentable Sugar Bioenergy Feedstock

“…For comparison, Figure b–d (lines) shows analogous derivative CVs of biomass portions (bagasse, leaf, and panicle from the same field experiments as juice) extracted in methanol or ethanol (20 g L –1 ); CV was obtained at pH 5 and 0.1 M KCl in 50 vol % solvent. The peak position of leaf (0.2–0.3 V in Figure c) matched the anodic peak of chlorophyll a in the literature (pH 7 in 1% acetone on carbon SPE) . Bagasse contained a peak at higher voltage (0.4–0.5 V in Figure b) indicative of less easily oxidized moieties.…”

Recent advances in portable heavy metal electrochemical sensing platforms

“…associated with a chemical reaction. 54 The use of electrochemistry for analytical purposes includes quality control, 55 environmental, 56,57 food 43,58 and biomedical 45,59 applications to name a few. These are typically dynamic processes that occur at the electrode-electrolyte interface.…”

Recent advances in 2D hexagonal boron nitride (2D-hBN) applied as the basis of electrochemical sensing platforms

“…The application of electrochemistry in analytical methods investigates changes in electrical properties that are related to chemical reactions/parameters. Electroanalysis has been widely explored for quality control [12], water [13] and environmental monitoring [14,15], forensics [16][17][18], food [19][20][21] and biomedical [22,23] applications to name just a few. In the application of 2D nanomaterials, they have reported to have significant benefits over other nanomaterials in the field of electroanalysis [8,24].…”