Voltammetric assay of rifampicin and isoniazid drugs, separately and combined in bulk, pharmaceutical formulations and human serum at a carbon paste electrode

Hammam, Essam; Beltagi, A. M.; Ghoneim, Mohammed M.

doi:10.1016/j.microc.2003.12.003

Cited by 69 publications

(30 citation statements)

References 51 publications

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“…Some analytical methods have been developed for the quantitative analysis of INH or PDX in pure individual drugs, in their combination, in the pharmaceutical dosage forms, and in the biological fluids. These methods are Fourier transform infrared [4], voltammetric assay [5,6], spectrophotometry ultraviolet (UV) [7][8][9][10][11][12][13][14][15], polarography [12] and chemiluminescence [13], reverse phase (RP)-liquid chromatography (LC) [14], RP-high-performance LC [15], and high-performance thin LC [16].…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Estimation of Isoniazid and Pyridoxine Hydrochloride in Tablet Dosage Form by Spectrophotometry Ultraviolet With Area Under Curve Method

Nasution

Bachri

Putra

2018

Asian J Pharm Clin Res

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Objective: This study aimed to develop spectrophotometry ultraviolet (UV) method by area under curve (AUC) to determine the levels of isoniazid (INH) and pyridoxine hydrochloride (PDX) in commercial tablets. Methods:Spectrophotometry UV method by AUC tested is carried out by optimizing the type of solvent, methanol, mixture of methanol, and phosphate buffer pH 4, pH 5, and pH 6 at a ratio of 90:10. The AUC value of each spectrum in various concentrations is calculated to selected wavelength range analysis. Then, this method was applied to determine the levels of INH and PDX in commercial tablets. Results:The application of AUC spectrophotometry UV method for determining the levels of INH and PDX was performed on wavelength range 262.2-272.2 nm and 289.8-299.8 nm, respectively. The selection of wavelengths based on wavelengths gives the best result. The mean % recoveries were found to be at 99.69% and 101.09% for INH and PDX, respectively. Conclusion:The method is successfully applied to analyze INH and PDX in pharmaceutical formulation simultaneously with no interference from excipients as indicated by all validation parameters which were within the acceptable range.

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Section: Introductionmentioning

confidence: 99%

Simultaneous Estimation of Isoniazid and Pyridoxine Hydrochloride in Tablet Dosage Form by Spectrophotometry Ultraviolet With Area Under Curve Method

Nasution

Bachri

Putra

2018

Asian J Pharm Clin Res

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“…Consequently, the need for a selective, precise, accurate, analytical method is urgent. The literature reveals quantitative spectrofluorometric and voltammetric methods 4,5 for the assay of RIF and INH, separately and combined in bulk, pharmaceutical formulations and human serum. Different analytical procedures for the simultaneous determination of the 4-FDC containing RIF, INH, PYP and EMB involving spectrophotometry [6][7][8][9][10] , and liquid chromatography [11][12][13][14][15][16][17] were reported.…”

Section: Introductionmentioning

confidence: 99%

A Novel Quantitative Method for the Simultaneous Assay of Rifampicin (RIF), Isoniazid (INH), Ethambutol (EMB), and Pyrazinamide (PYP) in 4-FDC Tablets

Hagga¹,

Sultana²

2016

Orient. J. Chem

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This paper presents the development and validation of a new ultra high performance chromatography (UHPLC) procedure for the simultaneous determination of isoniazid (INH), rifampicin (RIF), pyrazinamide (PYP), and ethambutol hydrochloride (EMB.2HCl) in four fixed-dose combination (4-FDC) anti-tubercular tablets. It is a dual wavelength single method in which, INH, RIF, and PYP were detected at 230 nm, whereas EMB was detected at 210 nm. The chromatographic separation was performed on Water's Acquity BEHC18 (50 mm x 2.1 mm, 1.7 µm particle size) column at a flow rate of 0.4 ml/min, 40 o C temperature, and a mobile phase consisting of triethylamine in phosphate buffer of pH 6.8, 95% and acetonitrile 5%. The proposed method was validated in terms of linearity, interday and intraday precision, recovery, specificity, and robustness. The results of the assay of the active constituents in two different batches of tablets were 102.3±1.5, 104.9±1.2, 101.0±1.4, 100.2±1.6, and 104.8±1.3, 103.8±1.1, 101.4±1.7, and 99.9±2.1, for INH, RIF, PYP, and EMB, respectively. The proposed method proves that the application of UHPLC technique is rewarding and additional economic benefits are gained as a result of reduction of analysis time and the consumption of the expensive HPLC grade materials.

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“…In case of electroanalysis used are stripping methods such as potentiometry [38,39], polarography [40,41], differential-pulse polarography [42,43] and stripping voltammetry [44][45][46][47][48][49]. The HMDE is the electrode of preference due to its high sensitivity, reproducibility and linearity.…”

Section: Introductionmentioning

confidence: 99%

Voltammetric Determination of Isoniazid using Cyclic Renewable Mercury Film Silver Based Electrode

2012

Pharm Anal Acta

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A new cathodic voltammetry method for the determination of isoniazid based on the cyclic renewable mercury film silver based electrode (Hg (Ag) FE) is presented. The effects of various factors such as: preconcentration potential and time, pulse height, step potential and supporting electrolyte composition are optimized. The calibration graph is linear from 5 nM up to 500 nM (68.55 µgL -1 ). For Hg (Ag) FE with a surface area of 9.7 mm 2 without time consuming preconcentration the limits of detection LOD and quantification LOQ were 4.1 nM and 10.5 nM of isoniazid, respectively. The repeatability of the method at a concentration level of the analyte as low as 0.5 nM, expressed as RSD is 3.6% (n=6). The proposed method was successfully applied in analysis of isonizid in simple and composed pharmaceutical formulations.

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Voltammetric assay of rifampicin and isoniazid drugs, separately and combined in bulk, pharmaceutical formulations and human serum at a carbon paste electrode

Cited by 69 publications

References 51 publications

Simultaneous Estimation of Isoniazid and Pyridoxine Hydrochloride in Tablet Dosage Form by Spectrophotometry Ultraviolet With Area Under Curve Method

Simultaneous Estimation of Isoniazid and Pyridoxine Hydrochloride in Tablet Dosage Form by Spectrophotometry Ultraviolet With Area Under Curve Method

A Novel Quantitative Method for the Simultaneous Assay of Rifampicin (RIF), Isoniazid (INH), Ethambutol (EMB), and Pyrazinamide (PYP) in 4-FDC Tablets

Voltammetric Determination of Isoniazid using Cyclic Renewable Mercury Film Silver Based Electrode

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