Voltage-sensitive dye imaging: Technique review and models

Chemla, Sandrine; Chavane, Frédéric

doi:10.1016/j.jphysparis.2009.11.009

Cited by 149 publications

(121 citation statements)

References 94 publications

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“…8), in which our knowledge of activity patterns is limited (Seelke and Blumberg, 2010). Notable weaknesses of VSD imaging include a limited contribution to the signal from deep layers of cortex (Berger, 2007;Chemla and Chavane, 2010;Mohajerani et al, 2010), particularly relevant because synaptic inputs to superficial layers 2/3 are weak during early life (Stern, 2001;Bureau, 2004). This weakness contributes to the more complex EEG signal compared with the VSD signal (Fig.…”

Section: Use Of Vsd To Study Developmental Activitymentioning

confidence: 99%

“…1C) (Devonshire, 2010). It is also worth noting that VSD signals can reflect subthreshold, dendritic depolarization (Grinvald and Hildesheim, 2004;Chemla and Chavane, 2010) making direct comparisons to patterns of calcium waves attributable to spiking (Schwartz, 1998;Garaschuk et al, 2000;Adelsberger et al, 2005) difficult (Berger, 2007). Nevertheless, it has been suggested recently that the diverse patterns of developmental activity previously reported are in fact all manifestations of spindle bursts under different experimental conditions (Khazipov and Buzaki, 2010), and in vivo VSD imaging may provide important clues to resolve this issue.…”

Section: Use Of Vsd To Study Developmental Activitymentioning

confidence: 99%

“…Nevertheless, dynamic spindle bursts have been reported (Khazipov et al, 2004, their supplemental figures), and they are synchronized between hemispheres (Yang et al, 2009). In this study, we directly study the dynamic properties of cortical activity in early life using voltage-sensitive dye (VSD) imaging to provides high spatiotemporal resolution signals of cortical activity (Grinvald and Hildesheim, 2004;Chemla and Chavane, 2010) from large regions of the cortex of 4-to 6-d-old rats in vivo. Although high-frequency patterns were not well represented in the VSD signal, we found prominent bursts of lower-frequency activity in sensory cortices.…”

Section: Introductionmentioning

confidence: 99%

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Voltage-Sensitive Dye Imaging Reveals Dynamic Spatiotemporal Properties of Cortical Activity after Spontaneous Muscle Twitches in the Newborn Rat

2012

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Spontaneous activity in the developing brain contributes to its maturation, but how this activity is coordinated between distinct cortical regions and whether it might reflect developing sensory circuits is not well understood. Here, we address this question by imaging the spread and synchronization of cortical activity using voltage-sensitive dyes (VSDs) in the developing rat in vivo. In postnatal day 4 -6 rats (n ϭ 10), we collected spontaneous changes in VSD signal that reflect underlying membrane potential changes over a large craniotomy (50 mm 2 ) that encompassed both the sensory and motor cortices of both hemispheres. Bursts of depolarization that occurred approximately once every 12 s were preceded by spontaneous twitches of the hindlimbs and/or tail. The close association with peripheral movements suggests that these bursts may represent a slow component of spindle bursts, a prominent form of activity in the developing somatosensory cortex. Twitch-associated cortical activity was synchronized between subregions of somatosensory cortex, which reflected the synchronized twitching of the limbs and tail. This activity also spread asymmetrically, toward the midline of the brain. We found that the spatial and temporal structure of such spontaneous cortical bursts closely matched that of sensory-evoked activity elicited via direct stimulation of the periphery. These data suggest that spontaneous cortical activity provides a recurring template of functional cortical circuits within the developing cortex and could contribute to the maturation of integrative connections between sensory and motor cortices.

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Section: Use Of Vsd To Study Developmental Activitymentioning

confidence: 99%

Section: Use Of Vsd To Study Developmental Activitymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Voltage-Sensitive Dye Imaging Reveals Dynamic Spatiotemporal Properties of Cortical Activity after Spontaneous Muscle Twitches in the Newborn Rat

2012

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“…While glia may contribute to the formation of IS initiation sites, the glial signals are relatively slow (Chemla and Chavane 2009) and unlikely to play a role in the short time frames where STDP occurs. In our experiments, glial membranes are also stained and are likely to contribute to the VSD signals.…”

Section: Discussionmentioning

confidence: 99%

Emergence of dominant initiation sites for interictal spikes in rat neocortex

Vitantonio

Geng

et al. 2015

Journal of Neurophysiology

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populations with unbalanced inhibition can generate interictal spikes (ISs), where each IS starts from a small initiation site and then spreads activation across a larger area. We used in vivo voltage-sensitive dye imaging to map the initiation site of ISs in rat visual cortex disinhibited by epidural application of bicuculline methiodide. Immediately after the application of bicuculline, the IS initiation sites were widely distributed over the entire disinhibited area. After ϳ10 min, a small number of sites became "dominant" and initiated the majority of the ISs throughout the course of imaging. Such domination also occurred in cortical slices, which lack long-range connections between the cortex and subcortical structures. This domination of IS initiation sites may allow timing-related plasticity mechanisms to provide a spatial organization where connections projecting outward from the dominant initiation site become strengthened. Understanding the spatiotemporal organization of IS initiation sites may contribute to our understanding of epileptogenesis in its very early stages, because a dominant IS initiation site with strengthened outward connectivity may ultimately develop into a seizure focus.

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“…Simulating those experiments is constrained to the presence of detailed and multi-scale volumetric models of the brain that are capable of addressing light interaction with the tissue including absorption and scattering. There are also other in silico experiments, such as voltage sensitive dye imaging [CC10] and calcium imaging [SGHK03], that require more complicated models to simulate fluorescence. These volumetric models must be annotated with the actual spectral characteristics of the fluorescent structures embedded in the tissue to reflect an accurate response upon excitation at specific input wavelength.…”

Section: Challenges and Related Studiesmentioning

confidence: 99%

Reconstruction and visualization of large-scale volumetric models of neocortical circuits for physically-plausible in silico optical studies

Abdellah

Hernando

Antille

et al. 2017

Preprint

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Background We present a software workflow capable of building large scale, highly detailed and realistic volumetric models of neocortical circuits from the morphological skeletons of their digitally reconstructed neurons. The limitations of the existing approaches for creating those models are explained, and then, a multi-stage pipeline is discussed to overcome those limitations. Starting from the neuronal morphologies, we create smooth piecewise watertight polygonal models that can be efficiently utilized to synthesize continuous and plausible volumetric models of the neurons with solid voxelization. The somata of the neurons are reconstructed on a physically-plausible basis relying on the physics engine in Blender.Results Our pipeline is applied to create 55 exemplar neurons representing the various morphological types that are reconstructed from the somatsensory cortex of a juvenile rat. The pipeline is then used to reconstruct a volumetric slice of a cortical circuit model that contains ∼210,000 neurons. The applicability of our pipeline to create highly realistic volumetric models of neocortical circuits is demonstrated with an in silico imaging experiment that simulates tissue visualization with brightfield microscopy. The results were evaluated with a group of domain experts to address their demands and also to extend the workflow based on their feedback.Conclusion A systematic workflow is presented to create large scale synthetic tissue models of the neocortical circuitry. This workflow is fundamental to enlarge the scale of in silico neuroscientific optical experiments from several tens of cubic micrometers to a few cubic millimeters.

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Voltage-sensitive dye imaging: Technique review and models

Cited by 149 publications

References 94 publications

Voltage-Sensitive Dye Imaging Reveals Dynamic Spatiotemporal Properties of Cortical Activity after Spontaneous Muscle Twitches in the Newborn Rat

Voltage-Sensitive Dye Imaging Reveals Dynamic Spatiotemporal Properties of Cortical Activity after Spontaneous Muscle Twitches in the Newborn Rat

Emergence of dominant initiation sites for interictal spikes in rat neocortex

Reconstruction and visualization of large-scale volumetric models of neocortical circuits for physically-plausible in silico optical studies

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