Voltage-induced conductance in human erythrocyte membranes

Kinosita, Kazuhiko; Tsong, Tian Yow

doi:10.1016/0005-2736(79)90386-9

Cited by 239 publications

(171 citation statements)

References 13 publications

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“…Because the membrane is incompressible, electrocompression leads to electrotension (ET) (Needham and Hochmuth 1989;Riske and Dimova 2005), which forms the basis of electroporation, a widely-used method by which molecules are inserted into cells through electric field-induced membrane pores (Weaver 1993(Weaver , 1995. Electroporation occurs when the transmembrane potential (TP) reaches 0.2-1.0 V (Akinlaja and Sachs 1998;Kinosita Jr. and Tsong 1979;Teissie et al 2005;Weaver 1993) resulting in membrane tensions exceeding 1 mN/m (Zhelev and Needham 1993). After pore formation, extracellular molecules enter the cell electrokinetically and diffusively.…”

Section: Introductionmentioning

confidence: 99%

Finite element analysis of microelectrotension of cell membranes

Bae

Butler

2007

Biomech Model Mechanobiol

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Electric fields can be focused by micropipette-based electrodes to induce stresses on cell membranes leading to tension and poration. To date, however, these membrane stress distributions have not been quantified. In this study, we determine membrane tension, stress, and strain distributions in the vicinity of a microelectrode using finite element analysis of a multiscale electro-mechanical model of pipette, media, membrane, actin cortex, and cytoplasm. Electric field forces are coupled to membranes using the Maxwell stress tensor and membrane electrocompression theory. Results suggest that micropipette electrodes provide a new non-contact method to deliver physiological stresses directly to membranes in a focused and controlled manner, thus providing the quantitative foundation for micreoelectrotension, a new technique for membrane mechanobiology.

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Section: Introductionmentioning

confidence: 99%

Finite element analysis of microelectrotension of cell membranes

Bae

Butler

2007

Biomech Model Mechanobiol

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“…Experimental results obtained either by monitoring conductance changes on cell suspension 34 or by fluorescence observation at the single cell level microscopy 28,29 shows that the local level of permeabilization is strongly controlled by the pulse duration. 27,28 As an electrical current is flowing, Joule heating is taking place.…”

Section: Critical Parameters Affecting Electropermeabilizationmentioning

confidence: 99%

Time dependence of electric field effects on cell membranes. A review for a critical selection of pulse duration for therapeutical applications

Teissié¹,

Escoffre²,

Rols³

et al. 2008

Radiology and Oncology

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Background. Electropulsation is one of the non-viral methods successfully used to transfer drugs and genes into living cells in vitro as in vivo. This approach shows promise in field of gene and cellular therapies. This presentation first describes the temporal factors controlling electropermeabilization to small molecules (< 4kDa) and then the processes supporting DNA transfer in vitro. The description of in vitro events brings our attention on the processes occurring before (s), during (ms) and after electropulsation (ms to hours) ofhas the main advantages of being easy to use, fast, reproducible and safe.While during 30 years due to technological limits, pulse duration was always larger than 1 microsecond, the recent availability of high voltage (tens of kV) nanosecond long pulse generators opens the way to a new approach. Very fast perturbations under strong fields are induced in the membrane organization. 16,17 A new field of development is now present for electropermeabilization and promising results for clinical applications were reported.One of the limiting problems remains that very few is known on the physicochemical mechanisms supporting the reorganisation of the cell membrane. The molecular target of the field effect remains unclear.The present review focuses on the critical role played by the pulse duration in the electropermeabilization to small molecules (< 4kDa) and on its support to the processes associated to DNA transfer in vitro. Pulse durations are easy to adjust for an optimization of the clinical target: electrochemotherapy, irreversible electropermeabilization or gene therapy as suggested as a final conclusion. Electropermeabilization Theory of membrane potential difference modulation.An external electric field modulates the membrane potential difference. 18 From the physical point of view, a cell can be described as a spherical capacitor which is charged by the external electrical field. The transmembrane potential difference induced by the electric field, ΔΨi is a complex function g(λ) of the specific conductivities of the membrane (λ m ), the pulsing buffer (λ out ) and the cytoplasm (λ cyt ), the membrane thickness and the cell size. Thus, 1 ΔΨi = f. g (λ). r. E.cosθ [1] in which θ designates the angle between the direction of the normal to the membrane at the considered point on the cell surface and the field direction, E the field intensity, r the radius of the cell and f, which is a shape factor (a cell being a spheroid). Therefore, ΔΨi is not uniform on the cell surface. It is maximum at the positions of the cell facing the electrodes. These physical predictions were checked experimentally by videomicroscopy by using the potential difference sensitive fluorescent probes. [19][20][21] The pulse duration plays a critical role when shorter than the capacitive loading time of the membrane. In the previous part of the paper, it was considered that the pulse was long enough to bring the potential steady state value. The loading time τ load brings a limit in this description. 1 ΔΨi = f. ...

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“…El principal efecto de los campos eléctricos en la membrana celular es, por lo tanto, causar permeabilidad debido a la compresión y formación de poros en ésta (Vega-Mercado et al, 1996b). Eanosita y Tsong (1977aTsong ( , 1979 demostraron que un campo eléctrico de 2.2 kV/cm inducía poros en eritrocitos humanos de aproximadamente 1 mm de diámetro (Martín y col., 1995). Kinosita y Tsong (1977a) sugirieron un mecanismo de dos pasos para la formación de los poros, en el cual el inicial es una respuesta a un potencial de campo eléctrico superior al umbral, seguido de una expansión del tamaño del poro en el tiempo, como se indica en la Fig.…”

Section: Mecanismos De Inactivación Microhiológica Por Medio De Campounclassified