Voltage-Gated Potassium Channels Ensure Action Potential Shape Fidelity in Distal Axons

Sabater, Victoria Gonzalez; Rigby, Mark R.; Burrone, Juan

doi:10.1523/jneurosci.2765-20.2021

Cited by 19 publications

(15 citation statements)

References 60 publications

(116 reference statements)

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“…A −100 mV to 50 mV depolarization triggered by the patch clamp generated the depolarization wave from the clamped cell to neighboring cells. With data interpolation at 1000-fold upsampling, ,− a slow radial propagation (defined by the delay of t 1/2 ) from the clamped cell to the 11 neighboring cells could be visualized at micrometer spatial resolution.…”

Section: Resultsmentioning

confidence: 99%

“…Compared to interpolation-based upsampling algorithms (such as SNAPT) at the data analysis end to boost the timing accuracy of action potential wavefronts, ,− SVI acquires image series at a higher temporal resolution over a larger FoV on the data acquisition end, thus providing a better starting point for SNAPT-based analysis. It is also worth noting that a stand-alone upsampling algorithm cannot eliminate the signal distortion introduced by low sampling rates and rolling shutter-related artifacts of the raw data in each pixel.…”

Section: Discussionmentioning

confidence: 99%

“…43,44,46 Notably, an alternative method to achieve fast imaging in larger FoV is sequentially capturing the adjacent smaller FoVs and stitching them together under the conventional illumination scheme. 31 To improve the temporal resolution by 5-fold, the acquisition FoV is reduced by a factor of 5. While this imaging scheme is easier to implement by avoiding the application of stroboscopic illumination, it is not as photon budget-efficient at SVI.…”

Section: All-optical Electrophysiology With Svimentioning

confidence: 99%

“…The method is adapted from the subframe interpolation approach to analyzing AP propagation from voltage imaging data. 9,[29][30][31]40 The SVI data of fluorescence fluctuation will first be denoised with a Gaussian spatial filter and principal components analysis (PCA)-based temporal filter. After excluding the pixel with a lower standard deviation in the frames, the linear interpolation will be performed on the rest pixel-of-interest, achieving 1000time upsampling.…”

Section: Linear Interpolation Of Subframe Timingmentioning

confidence: 99%

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Supertemporal Resolution Imaging of Membrane Potential via Stroboscopic Microscopy

Peng

Zou

2023

Chemical & Biomedical Imaging

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Membrane potential and its fluctuation are fundamental biophysical phenomena essential to cellular activities and functions. Compared to traditional electrode-based techniques, the optical recording via developed genetically encoded voltage indicators (GEVIs) offers a combination of noninvasiveness, high spatial resolution, and increased measurement throughput. However, its application is limited by the insufficient acquisition rate and time accuracy of the camera. Here we design and apply a stroboscopic illumination scheme to boost the temporal resolution of voltage imaging, while simultaneously eliminating the artifacts caused by nonsynchronized exposure in the rolling-shutter mode. We demonstrate that commonly used GEVIs are compatible with stroboscopic voltage imaging (SVI), and our SVI scheme offers a 5-fold faster acquisition frame rate than that of conventional continuous illumination. The GEVIs tested maintain high sensitivities in the SVI mode, supporting faithful reports of intracellular depolarization waveform and intercellular gap junction-mediated depolarization coupling in human embryonic kidney 293T (HEK 293T) cell populations. SVI allows resolving the action potential (AP) waveform with less distortion and mapping action potential initiation and propagation dynamics in cultured neurons in kilohertz, beyond the restriction from the camera in the field of view.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: All-optical Electrophysiology With Svimentioning

confidence: 99%

Section: Linear Interpolation Of Subframe Timingmentioning

confidence: 99%

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Supertemporal Resolution Imaging of Membrane Potential via Stroboscopic Microscopy

Peng

Zou

2023

Chemical & Biomedical Imaging

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“…To further probe these effects, we measured AP half-width during evoked spiking ( Figure 5 ). Differences in this measure may reflect changes to the mechanisms of AP generation, as AP shape is impacted by K+ currents ( Geiger and Jonas, 2000 ; Kole et al, 2007 ; Kuznetsov et al, 2012 ; Gonzalez Sabater et al, 2021 ; Alexander et al, 2022 ). Again, there was a dramatic difference in the effect of K + between the 4wk IOP and control groups: for both αON-S and αOFF-S, APs were less widened by high K + .…”

Section: Discussionmentioning

confidence: 99%

Retinal ganglion cells adapt to ionic stress in experimental glaucoma

et al. 2023

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IntroductionIdentification of early adaptive and maladaptive neuronal stress responses is an important step in developing targeted neuroprotective therapies for degenerative disease. In glaucoma, retinal ganglion cells (RGCs) and their axons undergo progressive degeneration resulting from stress driven by sensitivity to intraocular pressure (IOP). Despite therapies that can effectively manage IOP many patients progress to vision loss, necessitating development of neuronal-based therapies. Evidence from experimental models of glaucoma indicates that early in the disease RGCs experience altered excitability and are challenged with dysregulated potassium (K+) homeostasis. Previously we demonstrated that certain RGC types have distinct excitability profiles and thresholds for depolarization block, which are associated with sensitivity to extracellular K+.MethodsHere, we used our inducible mouse model of glaucoma to investigate how RGC sensitivity to K+ changes with exposure to elevated IOP.ResultsIn controls, conditions of increased K+ enhanced membrane depolarization, reduced action potential generation, and widened action potentials. Consistent with our previous work, 4 weeks of IOP elevation diminished RGC light-and current-evoked responses. Compared to controls, we found that IOP elevation reduced the effects of increased K+ on depolarization block threshold, with IOP-exposed cells maintaining greater excitability. Finally, IOP elevation did not alter axon initial segment dimensions, suggesting that structural plasticity alone cannot explain decreased K+ sensitivity.DiscussionThus, in response to prolonged IOP elevation RGCs undergo an adaptive process that reduces sensitivity to changes in K+ while diminishing excitability. These experiments give insight into the RGC response to IOP stress and lay the groundwork for mechanistic investigation into targets for neuroprotective therapy.

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Investigating Presynaptic Activity using the SF-iGluSnFR Sensor: Measuring Kinetics and Plasticity of Glutamate Release Across Heterogeneous Population of Synapses

Mendonça,

Langley,

Tagliatti

et al. 2024

Neuromethods

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Voltage-Gated Potassium Channels Ensure Action Potential Shape Fidelity in Distal Axons

Abstract: Title: Voltage-gated potassium channels ensure action potential shape fidelity in distal axons Abbreviated title: Potassium channels limit distal action potentials.

Cited by 19 publications

References 60 publications

Supertemporal Resolution Imaging of Membrane Potential via Stroboscopic Microscopy

Supertemporal Resolution Imaging of Membrane Potential via Stroboscopic Microscopy

Retinal ganglion cells adapt to ionic stress in experimental glaucoma

Investigating Presynaptic Activity using the SF-iGluSnFR Sensor: Measuring Kinetics and Plasticity of Glutamate Release Across Heterogeneous Population of Synapses

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