Voltage‐dependent synchronization of gating of syringomycin E ion channels

Ostroumova, Olga S.; Malev, V. V.; Kaulin, Yu.A.; Gurnev, Ph. A.; Takemoto, Jon Y.; Schagina, Ludmila V.

doi:10.1016/j.febslet.2005.08.087

Cited by 14 publications

(18 citation statements)

References 24 publications

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“…1, are commonly used to describe the kinetics of ion channels [15]. Collective action of such channels has been observed in numerous experiments [38][39][40], but the underlying mechanism by which the membrane proteins communicate is still not obvious.…”

Section: Discussionmentioning

confidence: 99%

Phase transitions and entropies for synchronizing oscillators

2016

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We study a generic model of coupled oscillators. In the model there is competition between phase synchronization and diffusive effects. For a model with a finite number of states we derive how a phase transition occurs when the coupling parameter is varied. The phase transition is characterized by a symmetry breaking and a discontinuity in the first derivative of the order parameter. We quantitatively account for how the synchronized pulse is a low-entropy structure that facilitates the production of more entropy by the system as a whole. For a model with many states we apply a continuum approximation and derive a potential Burgers' equation for a propagating pulse. No phase transition occurs in that case. However, positive entropy production by diffusive effects still exceeds negative entropy production by the shock formation.

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Section: Discussionmentioning

confidence: 99%

Phase transitions and entropies for synchronizing oscillators

2016

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“…It was shown that both the pore radius of small (elementary) channels included in clusters and their ionic selectivity are the same as those of elementary channels that are not included in clusters . Also, the conductances of the clusters are multiples (and integer values) of the conductance of elementary channels. ,, Studies show that the elementary SRE channel is an asymmetric peptide−lipid pore having a conical shape with the wider trans opening formed from host lipid molecules. , These channels are preferentially anion-permeable, and their conductance and kinetics of opening (or closure) are strongly voltage-dependent. ,,, The membrane conductance induced by SRE at fixed voltage increases with the sixth power of SRE concentration, suggesting that at least six toxin molecules are required for channel formation. ,, With negatively charged membranes bathed in 0.1 M NaCl at pH 6, the application of positive voltage opens the SRE channels. A reversal of the voltage sign leads to the closure of the channels.…”

Section: Introductionmentioning

confidence: 99%

Altering the Activity of Syringomycin E via the Membrane Dipole Potential

et al. 2008

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The membrane dipole potential is responsible for the modulation of numerous biological processes. It was previously shown (Ostroumova, O. S.; Kaulin, Y. A.; Gurnev, P. A.; Schagina, L. V. Langmuir 2007, 23, 6889-6892) that variations in the dipole potential lead to changes in the channel properties of the antifungal lipodepsipeptide syringomycin E (SRE). Here, data are presented demonstrating the effect of the membrane dipole potential on the channel-forming activity of SRE. A rise in the dipole potential is accompanied by both an increase in the minimum SRE concentration required for the detection of single channels at fixed voltage and a decrease in the steady-state number of open SRE channels at a given SRE concentration and voltage. These alterations are determined by several factors: gating charge, connected with translocations of lipid and SRE dipoles during channel formation, the bilayer-water solution partitioning of SRE, and the chemical work related to conformational changes during channel formation.

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“…It was shown that SyrE forms asymmetric peptide-lipid pores of a conical shape with predominant anion selectivity ( Malev et al, 2002 ; Ostroumova et al, 2007a ). The properties of single SyrE pores and lipopeptide induced steady-state transmembrane current depend on the boundary potential of membrane ( Feigin et al, 1996 ; Schagina et al, 1998 ; Dalla Serra et al, 1999 ; Ostroumova et al, 2005 , 2007b , 2008 ; Efimova et al, 2018b ). A previous study showed that a decrease in the membrane dipole potential upon addition of small molecule modifiers leads to a reduction of SyrE-pore conductance and dwell time and an increase in the SyrE-induced steady-state transmembrane current ( Ostroumova et al, 2007b , 2008 ; Efimova et al, 2018b ).…”

Section: Resultsmentioning

confidence: 99%

1,3-Thiazine, 1,2,3,4-Dithiadiazole, and Thiohydrazide Derivatives Affect Lipid Bilayer Properties and Ion-Permeable Pores Induced by Antifungals

Zakharova

Efimova

Yuskovets

et al. 2020

Front. Cell Dev. Biol.

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Over the past decade, thiazines, thiadiazoles, and thiohydrazides have attracted increasing attention due to their sedative, antimicrobial, antiviral, antifungal, and antitumor activities. The clinical efficacy of such drugs, as well as the possibility of developing resistance to antimicrobials, will depend on addressing a number of fundamental problems, including the role of membrane lipids during their interaction with plasma membranes. The effects of the eight 1,3-thiazine-, 1,2,3,4-dithiadiazole-, and thiohydrazide-related compounds on the physical properties of model lipid membranes and the effects on reconstituted ion channels induced by the polyene macrolide antimycotic nystatin and antifungal cyclic lipopeptides syringomycin E and fengycin were observed. We found that among the tested agents, the fluorine-containing compound N-(3,5-difluorophenyl)-benzenecarbothiohydrazide (C6) was the most effective at increasing the electric barrier for anion permeation into the hydrophobic region of the membrane and reducing the conductance of anion-permeable syringomycin pores. A decrease in the membrane boundary potential with C6 adsorption also facilitated the immersion of positively charged syringomycin molecules into the lipid bilayer and increases the pore-forming ability of the lipopeptide. Using differential scanning microcalorimetry, we showed that C6 led to disordering of membrane lipids, possibly by potentiating positive curvature stress. Therefore, we used C6 as an agonist of antifungals forming the pores that are sensitive to membrane curvature stress and lipid packing, i.e., nystatin and fengycin. The dramatic increase in transmembrane current induced by syringomycin E, nystatin, and fengycin upon C6 treatment suggests its potential in combination therapy for treating invasive fungal infections.

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Voltage‐dependent synchronization of gating of syringomycin E ion channels

Cited by 14 publications

References 24 publications

Phase transitions and entropies for synchronizing oscillators

Phase transitions and entropies for synchronizing oscillators

Altering the Activity of Syringomycin E via the Membrane Dipole Potential

1,3-Thiazine, 1,2,3,4-Dithiadiazole, and Thiohydrazide Derivatives Affect Lipid Bilayer Properties and Ion-Permeable Pores Induced by Antifungals

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