Voltage-dependent anion-selective channel 1 (VDAC1)—a mitochondrial protein, rediscovered as a novel enzyme in the plasma membrane

Lawen, Alfons; Ly, Jennifer; Lane, Darius J.R.; Zarschler, Kristof; Messina, Angela; Pinto, Vito De

doi:10.1016/j.biocel.2004.05.013

Cited by 63 publications

(50 citation statements)

References 15 publications

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“…Because increased mitochondrial membrane permeability would not be compatible with cell survival, it is proposed that plVDAC1 is not functional under normal conditions (Elinder et al, 2005). However, NADH-ferricyanide reductase activity of plVDAC1 has been observed in several cells types and in various physiological states (Baker et al, 2004;Lawen et al, 2005). Furthermore, plVDAC1 has been proposed to constitute the volume-regulated chloride channel (VRCC/VRAC) complex (Thinnes, 2007).…”

Section: Discussionmentioning

confidence: 98%

Hypothermia‐induced increase of oligodendrocyte precursor cells: Possible involvement of plasmalemmal voltage‐dependent anion channel 1

Imada

Yamamoto

Tanaka

et al. 2010

J of Neuroscience Research

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Hypothermia is believed to suppress cell proliferation by inducing apoptosis/necrosis and phase-specific/nonspecific cell cycle arrest, which are, directly or indirectly, related to a reduced energy supply. Intriguingly, hypothermia is known to improve neurological recovery of animals and humans exposed to focal brain hypoxic-ischemic injury. The underlying mechanism of the neuroprotective effect of hypothermia is unclear, although the prevention of neural cell apoptosis is thought to play a role. Herein we demonstrate that in vitro cell culture of oligodendrocyte precursor cells (OPCs) under conditions of mild hypothermia (31.5°C) results in an increase in cell number relative to cells cultured under normothermic conditions (37°C). Cell cycle analysis, immunoblotting of cyclins, TUNEL assay, and immunocytochemistry of OPC differentiation markers suggest that hypothermia shifts the balance between proliferation and apoptosis/differentiation toward proliferation. A combination of transcriptome analysis, pharmacological intervention, and immunoaffinity-based assays suggests a possible involvement of the Gα13-Rho GTPase Cdc42-ERK1/2 signaling cascade and voltage-dependent anion channel 1 (VDAC1), which associate or dissociate with Gα13 protein at 37°C and 31.5°C, respectively. Immunoelectron microscopy revealed the presence of VDAC1 in the plasma membrane of OPCs. Furthermore, the exogenous addition of impermeable VDAC1 inhibitors enhanced proliferation of OPCs at 37°C. These results may contribute to the elucidation of the mechanism of hypothermic neuroprotection as well as the possible novel role of plasmalemmal VDAC1.

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Section: Discussionmentioning

confidence: 98%

Hypothermia‐induced increase of oligodendrocyte precursor cells: Possible involvement of plasmalemmal voltage‐dependent anion channel 1

Imada

Yamamoto

Tanaka

et al. 2010

J of Neuroscience Research

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“…VDAC1 (isoform 1) is also found in the plasma membrane and was shown to be identical to NADH-ferricyanide reductase, which may be involved in the maintenance of cellular redox homeostasis [22]. Regulating this enzyme will impact on cancer and autoimmune diseases, as well as neurodegenerative diseases from the viewpoint of apoptosis [21].…”

Section: Discussionmentioning

confidence: 98%

“…All sera from four patients with MCTD were positive for the protein with 36 kDa obtained from HGECs, but sera from patients 3 and 4 were apparently negative for the protein with 36 kDa obtained from HMCs. Normal control sera were negative for this protein freely permeable to low molecular weight molecules [21]. VDAC1 (isoform 1) is also found in the plasma membrane and was shown to be identical to NADH-ferricyanide reductase, which may be involved in the maintenance of cellular redox homeostasis [22].…”

Section: Discussionmentioning

confidence: 99%

Human voltage-dependent anion selective channel 1 is a target antigen for antiglomerular endothelial cell antibody in mixed connective tissue disease

et al. 2008

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The purpose of this study was to identify the endothelial cell antigens that react with circulating antiendothelial antibody (AECA) in mixed connective tissue disease (MCTD). We screened serum AECA reactivity in 23 patients with MCTD using a human glomerular endothelial cell (HGEC) cellular ELISA. Proteomics, two-dimensional gel electrophoresis and matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry were used to identify the endothelial cell antigens of HGECs that reacted with serum antibodies from MCTD patients. Sera from 12 patients (52.0%) were positive for anti-HGEC antibody based on cellular ELISA. MALDI-TOF mass spectrometry used in combination with immunoblotting using serum antibody revealed one protein spot that represented a 36-kDa cell component of HGECs, with an isoelectric point (IP) of about 9, which had a high homology with the voltage-dependent anion-selective channel 1 (VDAC-1). This protein spot was confirmed to react with the antibody specific to VDAC-1. This is the first report of the presence of antibody to VDAC-1 from HGECs in the sera from MCTD patients. Although future studies will be needed to clarify the disease specificity of the a-VDAC-1 antibody in MCTD, the results show that modern proteomics technology is useful for identifying antigens that react with AECA in autoimmune diseases such as MCTD.

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“…VDAC-1 has recently been described as NADH: ferricyanide reductase in the plasma membrane. There it aVects the regulation of cell growth and death via apoptosis (reviewed in Lawen et al 2005). It has been reported that VDAC participates in amyloid beta-induced toxicity and interacts with plasma membrane estrogen receptor in septal and hippocampal neurons (Marin et al 2007).…”

Section: Transporters and Channelsmentioning

confidence: 98%

Proteomic analysis of the presynaptic active zone

Volknandt

Karas

2012

Exp Brain Res

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Synaptic vesicles are key organelles in chemical signaling, allowing neurons to communicate with each other and with neighboring cells. Vesicle integral or membrane-associated proteins mediate the various tasks the organelle fulfills during its life cycle. These include organelle transport, interaction with the nerve terminal cytoskeleton, uptake and storage of low molecular weight constituents, and the regulated interaction with the presynaptic plasma membrane, the active zone, during exo- and endocytosis. Converging work from several laboratories within the last 30 years resulted in the molecular and functional characterization of the protein inventory of the synaptic vesicle compartment. Nowadays advances in membrane protein separation and mass spectrometry have dramatically promoted this field resulting in a detailed description of the synaptic vesicle proteome and making synaptic vesicles the best characterized organelles. Recently, the proteome of the active zone was identified using the docked synaptic vesicles as target for immunoisolation. Combining gel-based protein separation techniques, mass spectrometry, and immunodetection, a considerable variety of proteins has been detected in the active zone. This includes synaptic vesicle proteins, components of the presynaptic fusion and retrieval machinery, proteins involved in intracellular signal transduction, a large variety of adhesion molecules and proteins potentially involved in regulating the functional and structural dynamics of the presynapse. Here, we discuss recent information concerning the proteome of the presynaptic active zone, focusing on proteins that are potentially involved in the short- and long-term structural modulation of the mature presynaptic compartment. In addition, we discuss the functional relevance of amyloid precursor protein in these membrane fractions and the putative interplay with direct or indirect interaction partners in the active zone.

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Voltage-dependent anion-selective channel 1 (VDAC1)—a mitochondrial protein, rediscovered as a novel enzyme in the plasma membrane

Cited by 63 publications

References 15 publications

Hypothermia‐induced increase of oligodendrocyte precursor cells: Possible involvement of plasmalemmal voltage‐dependent anion channel 1

Hypothermia‐induced increase of oligodendrocyte precursor cells: Possible involvement of plasmalemmal voltage‐dependent anion channel 1

Human voltage-dependent anion selective channel 1 is a target antigen for antiglomerular endothelial cell antibody in mixed connective tissue disease

Proteomic analysis of the presynaptic active zone

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