Voltage-activated and odor-modulated conductances in olfactory neurons ofDrosophila melanogaster

Dubin, Adrienne E.; Harris, Greg L.

doi:10.1002/(sici)1097-4695(199701)32:1<123::aid-neu11>3.0.co;2-l

Cited by 34 publications

(24 citation statements)

References 56 publications

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“…Although components of the cyclic nucleotide and phosphatidylinositol signaling pathway have been identified in Drosophila (22)(23)(24)(25), an essential role for either pathway has not emerged. The fact that the G␣ 15 subunit couples the activity of vertebrate seven-transmembrane receptors to the phospholipase C͞inositol 1,4,5-trisphosphate pathway (12,15), the functionality of this pathway in the heterologous system does not necessarily imply that this pathway functions in Drosophila ORNs in situ.…”

Section: Resultsmentioning

confidence: 99%

Functional expression and characterization of a Drosophila odorant receptor in a heterologous cell system

Wetzel

Behrendt²,

Gisselmann³

et al. 2001

Proc. Natl. Acad. Sci. U.S.A.

173

106

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Section: Resultsmentioning

confidence: 99%

Functional expression and characterization of a Drosophila odorant receptor in a heterologous cell system

Wetzel

Behrendt²,

Gisselmann³

et al. 2001

Proc. Natl. Acad. Sci. U.S.A.

173

106

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“…Although the well-established role of arrestins in receptor desensitization (30) might suggest that arrestin mutations would be expected to lead to some kinetic-signaling impairment, such an effect was not detected in these studies. To examine this effect, more sensitive means will be required including single-unit electrophysiology (31) or patch clamp recordings (32). It will be of interest to use these methods to examine the olfactory physiology in arrestin mutants to determine the molecular mechanism(s) that underlie these results, as several explanations seem plausible.…”

Section: Discussionmentioning

confidence: 99%

Visual arrestins in olfactory pathways of Drosophila and the malaria vector mosquito Anopheles gambiae

Merrill

Riesgo-Escovar

Pitts

et al. 2002

Proc. Natl. Acad. Sci. U.S.A.

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Arrestins are important components for desensitization of G proteincoupled receptor cascades that mediate neurotransmission as well as olfactory and visual sensory reception. We have isolated AgArr1, an arrestin-encoding cDNA from the malaria vector mosquito, Anopheles gambiae, where olfaction is critical for vectorial capacity. Analysis of AgArr1 expression revealed an overlap between chemosensory and photoreceptor neurons. Furthermore, an examination of previously identified arrestins from Drosophila melanogaster exposed similar bimodal expression, and Drosophila arrestin mutants demonstrate impaired electrophysiological responses to olfactory stimuli. Thus, we show that arrestins in Drosophila are required for normal olfactory physiology in addition to their previously described role in visual signaling. These findings suggest that individual arrestins function in both olfactory and visual pathways in Dipteran insects; these genes may prove useful in the design of control strategies that target olfactory-dependent behaviors of insect disease vectors.

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“…It might be possible to make intracellular recording from the component cells of scolopidia with an appropriate sharp electrode, perhaps quartz or by digesting the extracellular matrix with proteases. Our confidence that intracellular recordings can be made from Drosophila JO is bolstered by reports of whole-cell and perforated patch recordings from ORNs in A3s that have been cut open (Dubin and Harris, 1997;Cao et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

“…Although, it is now possible to patch-clamp Drosophila CNS in semi-intact preparations (Wilson et al, 2004), the exoskeleton has for the most part thwarted access to neurons and sensory structures buried within small cuticular compartments. Pioneering work by Dubin and Harris (1997) showed that it was possible to obtain patch clamp recordings from olfactory sensory neurons in Drosophila's third antennal segment (A3), cut open with iridectomy scissors. However, there is a need for a procedure that makes access simpler, more accurate and applicable to even smaller compartments.…”

Section: Introductionmentioning

confidence: 99%

Goggatomy: a Method for Opening Small Cuticular Compartments in Arthropods for Physiological Experiments

Kay

Raccuglia

Scholte

et al. 2016

Preprint

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Most sense organs of arthropods are ensconced in small exoskeletal compartments that hinder direct access to plasma membranes. We have developed a method for exposing live sensory and supporting cells in such structures. The technique uses a viscous light cured resin to embed and support the structure, which is then sliced with a sharp blade. We term the procedure a "goggatomy," from the Khoisan word for a bug, gogga. To demonstrate the utility of the method we show that it can be used to expose the auditory chordotonal organs in the second antennal segment and the olfactory receptor neurons in the third antennal segment of Drosophila melanogaster, preserving the transduction machinery. The procedure can also be used on other small arthropods, like mosquitoes and mites to expose a variety of cells.

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Voltage-activated and odor-modulated conductances in olfactory neurons ofDrosophila melanogaster

Cited by 34 publications

References 56 publications

Functional expression and characterization of a Drosophila odorant receptor in a heterologous cell system

Functional expression and characterization of a Drosophila odorant receptor in a heterologous cell system

Visual arrestins in olfactory pathways of Drosophila and the malaria vector mosquito Anopheles gambiae

Goggatomy: a Method for Opening Small Cuticular Compartments in Arthropods for Physiological Experiments

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